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作物学报 ›› 2015, Vol. 41 ›› Issue (03): 367-377.doi: 10.3724/SP.J.1006.2015.00367

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用SRAP、ISSR、SSR标记绘制黄麻基因源分子指纹图谱

巫桂芬1,2，徐鲜均3，徐建堂1，陶爱芬1，张立武1，魏丽真4，潘漠5，方平平1，林荔辉1，祁建民1,*   

  1. 1 福建农林大学 / 作物遗传育种与综合利用教育部重点实验室 / 福建省作物设计育种重点实验室 /福建省南方特色经济作物遗传育种与多用途综合利用国际合作基地，福建福州350002；2 广西农业科学院园艺研究所，广西南宁530007；3 武夷山生物资源研究所，福建武夷山 3543004；4 福州市第三中学，福建福州 350002；5 福州天地同人信息科技有限公司，福建福州350001
  • 收稿日期:2014-09-14 修回日期:2014-12-19 出版日期:2015-03-12 网络出版日期:2015-01-12
  • 通讯作者: 祁建民, E-mail: qijm863@163.com, Tel: 0591-87644898
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-19-E06)，引进国际先进农业科学技术计划(948计划)项目(2013-Z7)和国家科技支撑计划项目(2013BAD01B03-13)资助。

Construction of Molecular Fingerprinting Map in Gene Pool of Jute with SRAP, ISSR and SSR Markers

WU Gui-Fen1,2,XU Xian-Jun3,XU Jian-Tang1,TAO Ai-Fen1,ZHANG Li-Wu1,WEI Li-Zhen4,PAN Mo5,FANG Ping-Ping1,LIN Li-Hui1,QI Jian-Min1,*   

  1. 1 Key Laboratory of Ministry for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Province International Cooperation Base for Genetics, Breeding and Multiple Utilization of Characteristic Economy Crops in South China , Fuzhou 350002, China; 2 Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3 Wuyishan Biological Resource Institute of Fujian Province, Fuzhou 354300, China; 4 Third Middle School of Fuzhou, Fuzhou 350002, China; 5 Fuzhou Tanditongren Mdt InfoTech Ltd, Fuzhou 350001, China
  • Received:2014-09-14 Revised:2014-12-19 Published:2015-03-12 Published online:2015-01-12
  • Contact: 祁建民, E-mail: qijm863@163.com, Tel: 0591-87644898

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被引次数

28

摘要：

以国内外引进保存的231份黄麻种质资源为材料，分别采用SRAP、ISSR、SSR分子标记和编程DNA指纹图谱分析软件，绘制黄麻遗传资源基因组DNA指纹图谱。结果表明，通过筛选出的35对SRAP引物对231份黄麻品种进行标记分析，获得96份黄麻品种DNA指纹图谱；11个ISSR多态性引物对96份材料进行DNA标记分析，获得45份黄麻品种DNA指纹图谱；49对SSR多态性引物对48份黄麻品种进行DNA标记分析，获得13份黄麻品种DNA指纹图谱，累计完成了154份黄麻品种基因组DNA分子指纹图谱绘制。每一个被识别的品种都具有其独特的分子“身份证”。其他77份地方品种因与部分品种遗传相似性过高，未能被识别，表明黄麻地方品种存在较为严重的同种异名现象。

关键词: 黄麻, 遗传资源, 分子标记, DNA指纹图谱

Abstract:

An experiment was conducted using 231 jute germplasm resources from abroad and at home to construct DNA fingerprints of jute varieties with SRAP, ISSR, SSR marker and the DNA fingerprint analysis software. The results showed that 96 DNA fingerprints from 231 jute germplasm resources with 35 pairs of selected SRAP primers, 45 DNA fingerprints from 96 jute varieties with 11 selected polymorphic ISSR primers, and 13 DNA fingerprints from 48 jute varieties with 49 selected polymorphic SSR primers were constructed. This study completed a total of 154 genomic DNA molecular fingerprint maps of jute varieties. Every identified jute variety had its unique “ID”. Other 77 local varieties had not been able to be identified due to their high genetic similarity with some varieties. It showed that jute local variety has a serious phenomenon of synonym.

Key words: Jute, Genetic resources, Molecular markers, DNA fingerprint

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