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作物学报 ›› 2015, Vol. 41 ›› Issue (03): 386-393.doi: 10.3724/SP.J.1006.2015.00386

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

云南3种野生稻中抗白叶枯病基因的鉴定

李定琴,陈玲,李维蛟,柯学,余腾琼,李娥贤,黄兴奇,程在全*   

  1. 云南省农业科学院生物技术与种质资源研究所 / 云南省农业生物技术重点实验室 / 农业部西南作物基因资源与种质创制重点实验室,云南昆明 650223
  • 收稿日期:2014-09-23 修回日期:2014-12-19 出版日期:2015-03-12 网络出版日期:2015-01-12
  • 通讯作者: 程在全,E-mail: czquan-99@163.com
  • 基金资助:

    本研究由国家自然科学基金项目(31000746, 31160067, U1302265),国家公益性行业(农业)科研专项(201003021),云南省高端人才项目(2012HA002)和云南省应用基础研究项目(2010CD098)资助。

Identification of Bacterial Blight Resistance Gene in Yunnan Wild Rice

LI Ding-Qin,CHEN Ling,LI Wei-Jiao,KE Xue,YU Teng-Qiong,LI E-Xian,HUANG Xing-Qi,CHENG Zai-Quan*   

  1. 1Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences / Yunnan Provincial Key Laboratory of Agricultural Biotechnology / Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650223, China
  • Received:2014-09-23 Revised:2014-12-19 Published:2015-03-12 Published online:2015-01-12
  • Contact: 程在全,E-mail: czquan-99@163.com

摘要:

白叶枯病是水稻生产上最重要的细菌性病害之一,培育抗病新品种是防治白叶枯病最经济有效的途径。然而,栽培稻来源的抗病基因数量有限,并且部分抗病基因的抗病谱窄。因此,从野生稻中发掘抗病基因,将有利于培育抗病谱广且抗病能力强的水稻新品种。云南野生稻资源丰富,但其中是否含有已报道克隆的抗白叶枯病基因,目前还未见相关综合研究报道。本研究通过抗性鉴定和PCR分析,检测云南野生稻中的抗白叶枯病基因。结果表明,云南野生稻对两个代表性白叶枯病菌Y8和PXO99具有不同程度的抗性,疣粒野生稻甚至达到免疫的程度。3种野生稻中均不含xa5xa13Xa21抗病基因,元江普通野生稻含Xa23Xa3/Xa26基因或其同源基因,景洪普通野生稻中含Xa1Xa3/Xa26Xa27基因或同源基因,药用野生稻含Xa3/Xa26基因或同源基因,而疣粒野生稻含有Xa27抗性基因。本研究结果为进一步发掘和克隆云南野生稻中的抗白叶枯病新基因提供了理论参考。

关键词: 野生稻, 白叶枯病, 抗性基因, PCR鉴定

Abstract:

Bacterial blight (BB) is one of the most destructive diseases in rice production. The most efficient and economical way of controlling the disease is developing disease resistance varieties. Owing to the limited number of resistance genes from cultivated rice and the narrow spectrum of some bacterial blight resistance genes, exploring new resistance genes from wild rice will be conducive to development of new resistance varieties with wider spectrum and higher resistance. Yunnan is rich in resources of wild rice. However, till now there are few reports concerning whether they contain seven cloned BB resistance genes. In this study, seven cloned BB genes were detected in Yunnan wild rice by resistance identification and PCR analysis. The results showed that Yunnan wild rice had different levels of resistance to BB strains Y8 and PXO99, and O. granulata was immune to Y8 and PXO99. The resistance genes xa5, xa13 and Xa21 were not found in all three wild rice materials. Xa1 was detected in O. rufipogon type from Jinghong. Xa3/Xa26 was identified in O. rufipogon types from Yuanjiang and Jinghong, respectively. Xa3/Xa26 was alsoidentified in O. officinalis, Xa23 was found in O. rufipogon type from Yuanjiang, Xa27 in O. rufipogon type from Jinghong, and Xa27 in O. granulata. The results provided a theoretical reference for further exploring and cloning new BB resistance genes in Yunnan wild rice to improve the disease resistance of cultivated rice.

Key words: Wild rice, Bacterial blight, Resistance gene, PCR identification

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