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作物学报 ›› 2008, Vol. 34 ›› Issue (10): 1757-1761.doi: 10.3724/SP.J.1006.2008.01757

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

莫迦小麦(Triticum macha L.) T型恢复基因Rf3和K型不育基因rfv1的连锁关系

宋喜悦1;董普辉2;胡银岗1;马翎健1;李宏斌1;何蓓如1,*   

  1. 1西北农林科技大学农学院, 陕西杨凌712100; 2 河南科技大学农学院, 河南洛阳475300
  • 收稿日期:2007-11-22 修回日期:1900-01-01 出版日期:2008-10-12 网络出版日期:2008-10-12
  • 通讯作者: 何蓓如

Linkage Relationship between T-type Restorer Gene Rf3 and K-type Male Sterile Gene rfv1 in Triticum macha L.

SONG Xi-Yue1,DONG Pu-Hui2,HU Yin-Gang1,MA Ling-Jian1,LI Hong-Bin1,HE Bei-Ru1*   

  1. 1 College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi; 2 College of Agronomy, Henan University of Science and Technology, Luoyang 471003, Henan, China
  • Received:2007-11-22 Revised:1900-01-01 Published:2008-10-12 Published online:2008-10-12
  • Contact: HE Bei-Ru

摘要: 为进一步明确莫迦小麦(Triticum macha) T型恢复基因Rf3与K型不育基因rfv1的连锁关系, 利用T型细胞质背景(T504A/Tm3314 F2代和T504A//KTm3314A/90(13)21杂交分离群体)的可育株在K型细胞质下的育性测交分析, 明确了来自莫迦小麦的这2个基因连锁并不紧密, 交换值约为16.54%。可利用T型主效恢复基因Rf3提高含有T型主效恢复基因和K型主效不育基因的基础材料的选择效率。

关键词: Triticum macha, T型主效恢复基因Rf3, K型主效不育基因rfv1

Abstract: Wheat (Triticum aestivum L.) breeding line Tm3314 is a T-type non-1B/1R material carrying 1BS of T. macha. KTm3314A, a K-type non-1B/1R male sterile line developed from Tm3314, is easily restored in fertility, does not produce haploids in reproduction, and has high temperature-sensitivity, thus has potential in wheat hybrid breeding. The T-type restorer gene Rf3, which is closely linked to K-type male sterile gene rfv1 on 1BS in T. spelta, is used as a marker of rfk1 under the background of T. timophvee cytoplasm. However, some male sterile materials with substitutive chromosome fragments of the 1BS of T. macha have been found the absence of rfv1 under T. timophvee cytoplasm background. To understand the linkage relationship between Rf3 and rfv1 in T. macha, the segregation generations of (T504A/Tm3314) F2 and T504A//KTm3314A/90(13)21 as well as their backcross populations with a K-type sterile line K119A were used in field experiments in 2003–2006. The results showed that crossing-over value between Rf3 and rfv1 was 16.54%.

Key words: Triticum macha L., Major T-type restorer gene Rf3, Major K-type male sterile gene rfv1

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