欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2008, Vol. 34 ›› Issue (10): 1762-1769.doi: 10.3724/SP.J.1006.2008.01762

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

烤烟几种化学成分的QTL初步分析

肖炳光1;卢秀萍1;焦芳蝉1;李永平1;孙玉合2;郭兆奎3   

  1. 1中国烟草育种研究(南方)中心 / 云南省烟草科学研究所, 云南玉溪 653100; 2中国农业科学院烟草研究所, 山东青岛 266101; 3 黑龙江省烟草科学研究所, 黑龙江牡丹江 157011
  • 收稿日期:2007-12-12 修回日期:1900-01-01 出版日期:2008-10-12 网络出版日期:2008-10-12
  • 通讯作者: 肖炳光

Preliminary QTL Analysis of Several Chemical Components in Flue- Cured Tobacco (Nicotiana tabacum L.)

XIAO Bing-Guang1,LU Xiu-Ping1,JIAO Fang-Chan1,LI Yong-Ping1,SUN Yu-He2,GUO Zhao-Kui3   

  1. 1 China Tobacco Breeding Research (Southern) Center / Yunnan Institute of Tobacco Science, Yuxi 653100, Yunnan; 2 Institute of Tobacco Science, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong; 3 Heilongjiang Institute of Tobacco Science, Mudanjiang 157011, Heilong- jiang, China
  • Received:2007-12-12 Revised:1900-01-01 Published:2008-10-12 Published online:2008-10-12
  • Contact: XIAO Bing-Guang

PDF

1528

被引次数

66

摘要： 以含137个株系的烤烟DH群体(G-28×NC2326)及其亲本为材料, 在以前作图数据的基础上, 新增23个标记。将这些标记数据合并起来构建了包括11个ISSR标记和158个RAPD标记、由27个连锁群组成的烤烟分子标记遗传连锁图, 覆盖长度2 094.6 cM, 相邻标记间的平均图距为15.95 cM。利用4个环境下的试验数据进行了总糖、烟碱、氧化钾3种烟叶化学成分的QTL初步分析, 共检测到7个加性效应QTL和9对加加上位性效应QTL, 其中3个加性QTL和3对上位性QTL存在QTL与环境互作效应(QE)。表明在烤烟总糖、烟碱、氧化钾的遗传控制中除加性效应外, 上位性效应也具有重要作用。对于烟碱、氧化钾检测到加性QTL与环境互作效应, 对于总糖、氧化钾检测到上位性QTL与环境互作效应, 利用这些与环境具有互作效应的QTL进行标记辅助选择时宜考虑特定的环境条件。

关键词: 烤烟, 遗传连锁图, 化学成分, QTL分析, 上位性, QTL与环境互作

Abstract: The chemical components are the important traits influencing the leaf quality of tobacco. QTLs linked to the chemical components can be used for marker-assisted selection in leaf quality improvement in tobacco. The objective of this study was to identify QTLs linked to several chemical components in the flue-cured tobacco. A population of 137 doubled haploid (DH) lines derived from a cross between two flue-cured tobacco cultivars G-28 and NC2326 was employed to construct the genetic linkage map. Twenty-three new markers were obtained by ISSR and RAPD analysis, and were combined with the previous marker data for the construction of linkage map. A genetic linkage map consisting of 11 ISSR markers and 158 RAPD markers was developed. The map consisted of 27 linkage groups and spanned 2 094.60 cM with an average distance of 15.95 cM between adjacent markers. The data for three chemical components including total sugar (TS), nicotine (NIC) and potassium oxide (KO) were collected from a field experiment conducted in four different environments, and a full-QTL model and the corresponding mapping software were used for the preliminary QTL analysis. Seven additive effect QTLs and nine pairs of epistatic QTLs were detected. The results indicated that epistasis played important roles in the genetic basis of the chemical components in the flue-cured tobacco. For the epistatic QTLs, in which the individual QTL showed no additive effect, it should be efficient to select the QTL combinations rather than the single QTL. Three additive QTLs and three pairs of epistatic QTLs were found to involve in QTL-by-environment interactions (QE): The additive QTL-by-environment interactions were detected for NIC and KO, and the epistatic QTL-by-environment interactions were detected for TS and KO. QTL-by-environment interactions presumably arise from the differential gene expression in various environments and their application in marker-assisted breeding programs would require careful consideration with regard to a particular set of environmental conditions. The present study provides a starting point for detecting QTLs for economically important traits and for understanding the genetic basis of the traits.

Key words: Flue-cure tobacco, Genetic linkage map, Chemical component, QTL analysis, Epistasis, QTL-by-environment interaction

[1] 罗兰, 雷丽霞, 刘进, 张瑞华, 金桂秀, 崔迪, 黎毛毛, 马小定, 赵正武, 韩龙植. 利用东乡普通野生稻染色体片段置换系定位产量相关性状QTL[J]. 作物学报, 2021, 47(7): 1391-1401.
[2] 周新桐, 郭青青, 陈雪, 李加纳, 王瑞. GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状[J]. 作物学报, 2021, 47(4): 587-598.
[3] 刘青丽,蒋雨洲,邹焱,张云贵,张恒,石俊雄,李志宏. 烟田生态系统碳收支研究[J]. 作物学报, 2020, 46(8): 1258-1265.
[4] 刘荣, 王芳, 方俐, 杨涛, 张红岩, 黄宇宁, 王栋, 季一山, 徐东旭, 李冠, 郭瑞军, 宗绪晓. 利用2个F2群体整合中国豌豆高密度SSR遗传连锁图谱[J]. 作物学报, 2020, 46(10): 1496-1506.
[5] 赵松超,李一凡,刘博远,赵铭钦. 晾制密度对雪茄烟叶膜脂过氧化作用及品质的影响[J]. 作物学报, 2019, 45(7): 1090-1098.
[6] 赵小强,任斌,彭云玲,徐明霞,方鹏,庄泽龙,张金文,曾文静,高巧红,丁永福,陈奋奇. 8种水旱环境下2个玉米群体穗部性状QTL间的上位性及环境互作效应分析[J]. 作物学报, 2019, 45(6): 856-871.
[7] 童治军,张谊寒,陈学军,曾建敏,方敦煌,肖炳光. 雪茄烟品种Beinhart1000-1赤星病抗性基因的QTL定位[J]. 作物学报, 2019, 45(3): 477-482.
[8] 李成江,李大肥,周桂夙,许龙,徐天养,赵正雄. 不同种类生物炭对植烟土壤微生物及根茎病害发生的影响[J]. 作物学报, 2019, 45(2): 289-296.
[9] 周健飞,武云杰,薛刚,张安乾,田培,彭玉富,杨铁钊. 烤烟成熟期烟叶GS同工酶活性与氮素运转的关系[J]. 作物学报, 2019, 45(1): 111-117.
[10] 艾丽娟,陈强,杨春燕,闫龙,王凤敏,葛荣朝,张孟臣. 大豆籽粒硬实加性和上位性QTL定位[J]. 作物学报, 2018, 44(6): 852-858.
[11] 沈杰,蔡艳,何玉亭,李启权,杜宣延,王昌全,罗定棋. 基于归一化法的烤烟干物质积累建模与特征分析[J]. 作物学报, 2017, 43(03): 442-453.
[12] 吕品,于海峰,于志贤,张永虎,张艳芳,王婷婷,侯建华. 向日葵高密度遗传连锁图谱构建及两种水分条件下芽期性状的QTL分析[J]. 作物学报, 2017, 43(01): 19-30.
[13] 陈强**,闫龙**,邓莹莹,肖二宁,刘兵强,杨春燕*,张孟臣*. 大豆籽粒大小与形状性状的QTL定位[J]. 作物学报, 2016, 42(09): 1309-1318.
[14] 冯建英,温阳俊,张瑾,章元明. 植物关联分析方法的研究进展[J]. 作物学报, 2016, 42(07): 945-956.
[15] 梁慧珍,余永亮,杨红旗,董薇,许兰杰,牛永光,张海洋,刘学义,方宣钧. 大豆叶片性状和叶绿素含量QTL间的上位性和环境互作效应[J]. 作物学报, 2015, 41(06): 889-899.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2