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作物学报 ›› 2017, Vol. 43 ›› Issue (01): 19-30.doi: 10.3724/SP.J.1006.2017.00019

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

向日葵高密度遗传连锁图谱构建及两种水分条件下芽期性状的QTL分析

吕品1,于海峰2,于志贤1,张永虎2,张艳芳2,王婷婷1,侯建华1,*   

  1. 1内蒙古农业大学，内蒙古呼和浩特 010019; 2内蒙古农牧业科学院, 内蒙古呼和浩特 010031?
  • 收稿日期:2016-03-09 修回日期:2016-09-18 出版日期:2017-01-12 网络出版日期:2016-09-28
  • 通讯作者: 侯建华, E-mail: houjh68@163.com
  • 基金资助:

    本研究由国家自然科学基金项目(31160288, 31540043)资助。

Construction of High-density Genetic Map and QTL Mapping for Seed Germination Traits in Sunflower under Two Water Conditions

LYU Pin1,YU Hai-Feng2,YU Zhi-Xian1,ZHANG Yong-Hu2,ZHANG Yan-Fang2,WANG Ting-Ting1,HOU Jian-Hua1,*   

  1. 1 Inner Mongolia Agricultural University, Huhhot 010010, China; 2 Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Huhhot 010031, China
  • Received:2016-03-09 Revised:2016-09-18 Published:2017-01-12 Published online:2016-09-28
  • Contact: 侯建华, E-mail: houjh68@163.com
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (31160288, 31540043).

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摘要：

干旱胁迫对向日葵发芽出苗有重要影响。以K55×K58组合衍生的187个F6重组自交系为材料，利用SSR、SRAP、AFLP标记构建向日葵高密度遗传连锁图谱，设置正常水分(CK)和模拟干旱(18%聚乙二醇PEG-6000)两种水分条件，调查9个芽期数量性状，PCR扩增株系，构建一张包含17个连锁群、1105个标记(368个SSR、368个SRAP和369个AFLP)的高密度遗传连锁图谱。该图谱覆盖基因组长度3846.0 cM，平均图距3.48 cM，连锁群长度147.6~295.5 cM，每个连锁群标记数10~165个。两种条件下检测到33个QTL，其中干旱条件下检测到发芽指数、发芽率、胚芽长、胚根长、胚芽鲜重和胚根鲜重6个性状的14个QTL，可解释6.1%~14.0%的表型变异；正常水分(CK)条件下检测到发芽势、胚根长、胚芽鲜重、胚根鲜重、胚根干重和胚芽干重6个性状的19个QTL，可解释6.1%~25.8%的表型变异。两种水分条件下检测到Qefw5-1、Qefw5-2、Qefw5-4、Qrfw5、Qrfw10和Qrl9共6个QTL的遗传贡献率超过10%，此外，还检测到9个影响干旱胁迫与正常水分条件下性状差值的QTL，可能对抗旱性有直接贡献。这些QTL可为向日葵芽期抗旱分子设计育种研究提供重要参考。

关键词: 向日葵, 遗传连锁图谱, 耐旱性, 芽期性状, QTL

Abstract:

Seed germination and emergence of sunflower are seriously affected by water stress. In this study, SSR, SRAP, and AFLP markers were applied to construct a genetic linkage map by using the F6 population derived from a cross of K55 (drought sensitive) ´ K58 (drought resistant). For mapping quantitative trait loci (QTLs) for nine traits of seed germination in sunflower,the parents and 187 F6 family lines were used to investigate seed germination traits under normal condition (CK) and 18% polyethylene glycol (PEG-6000) (drought stress). A genetic map consisting of 17 linkage groups was constructed with 1105 loci (368 SSR, 369 SRAP, 333 AFLP) which covers 3846.0 cM and the length of each linkage group varies from 147.6 to 295.5 cM, the number of markers in each linkage group varies from 10 to 165 with an average distance of 3.481 cM. As a result, a total of 33 QTLs were detected. We identified fourteen additive QTLs for germination index (GI), germination rate (GR), embryo length (EL), radicle length (RL), embryo fresh weight (EFW), radicle fresh weight (RFW) under18% PEG condition with explained phenotypic variance ranging from 6.1% to 14.0%. Nineteen additive QTLs were identified for germination energy(GE), radicle length (RL), embryo fresh weight (EFW), radicle fresh weight (RFW), embryo dry weight (EDW), radicle dry weight (RDW) under normal condition with explained phenotypic variance ranging from 6.1% to 25.8%. Each ofQefw5-2, Qefw5-1, Qefw5-4, Qrfw10, Qrfw5, and Qrl9could explain phenotypic variance over 10%. Nine QTLs affecting trait differences between stress treatment and control were identified, which are considered to directly contribute to drought tolerance. These QTLs identified could provide important reference to molecular breeding for drought-resistance during seed germination in sunflower.

Key words: Sunflower, Genetic linkage map, Drought tolerance, Seed germination traits, QTL

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