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张瀚,余金琎,谭林璐,张婧泉,王小董,谢庄,谢可盈,凌英华,赵芳明*
ZHANG Han,YU Jin-Jin,TAN Lin-Lu,ZHANG Jing-Quan,WANG Xiao-Dong,XIE Zhuang,XIE Ke-Ying, LING Ying-Hua,ZHAO Fang-Ming*
摘要:
水稻产量性状作为典型的数量性状,受微效多基因系统调控。将相关基因解析至单片段代换系(SSSL)不仅为深入阐明其分子机制提供了理想研究体系,更因克服遗传背景干扰的优势,为基于SSSL平台的全基因组设计育种奠定了重要基础。本研究以日本晴为遗传背景的染色体片段代换系CSSL Z267 (携带5个供体片段)为材料,通过构建日本晴/Z267的F2群体,成功鉴定出9个调控产量性状的QTL,并进一步通过遗传解析获得5个单片段代换系(SSSL)和1个双片段代换系(DSSL)。研究发现,S1~S5均携带显著增加粒长和二次枝梗数的正向效应QTL,同时具有降低粒宽的负向效应QTL。在双片段代换系D1中观察到多对QTL的互作效应:穗长(qPL6与qPL1)、一次枝梗数(qNPB6与qNPB1)和二次枝梗数(qNSB1与qNSB6)位点聚合均产生超亲遗传效应;粒宽(qGW6与qGW1)和千粒重(qGWT6与qGWT1)位点组合则表现出亚显性效应;而粒长(qGL6与qGL1)和株高(qPH6与qPH1)位点组合的遗传效应分别与qGL1和qPH6单一位点相当。遗传效应解析表明,S1与S5的杂交组合可有效选育出株型较高且籽粒细长化的优良株系。本研究系统解析了产量相关QTL的遗传效应,为阐明其分子机制和推进水稻全基因组设计育种提供了重要理论依据和材料基础。
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