水稻幼穗分化期至抽穗期高温对籽粒形态和充实的影响及其与粒重的关系

doi:10.3724/SP.J.1006.2025.42046

作物学报 ›› 2025, Vol. 51 ›› Issue (5): 1347-1362.doi: 10.3724/SP.J.1006.2025.42046

水稻幼穗分化期至抽穗期高温对籽粒形态和充实的影响及其与粒重的关系

王梦宁(), 谢可冉, 高逖, 王飞, 任孝俭, 熊栋梁, 黄见良, 彭少兵, 崔克辉^*()

作物遗传改良国家重点实验室 / 农业农村部长江中游作物生理生态与耕作重点实验室 / 华中农业大学植物科学技术学院, 湖北武汉 430070

收稿日期:2024-10-24 接受日期:2025-01-23 出版日期:2025-05-12 网络出版日期:2025-02-11
通讯作者: *崔克辉, E-mail: cuikehui@mail.hzau.edu.cn
作者简介:E-mail: 18439406270@163.com
基金资助:
国家自然科学基金项目(31871541);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-01)

Effect of high temperature during the panicle initiation and heading stages on grain shape and filling and its relationship with grain weight in rice

WANG Meng-Ning(), XIE Ke-Ran, GAO Ti, WANG Fei, REN Xiao-Jian, XIONG Dong-Liang, HUANG Jian-Liang, PENG Shao-Bing, CUI Ke-Hui^*()

National Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, Ministry of Agricultural and Rural Affairs / College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China

Received:2024-10-24 Accepted:2025-01-23 Published:2025-05-12 Published online:2025-02-11
Contact: *E-mail: cuikehui@mail.hzau.edu.cn
Supported by:
National Natural Science Foundation of China(31871541);China Agriculture Research System of MOF and MARA(CARS-01)

摘要/Abstract

摘要：

以热敏感型品种两优培九(LYPJ)和晶两优华占(JLYHZ)、耐热型品种汕优63 (SY63)为材料, 在盆栽条件下设置幼穗分化期至抽穗期常温和高温2种温度处理, 探究幼穗分化期至抽穗期高温对穗分化期颖花和籽粒大小、灌浆期籽粒充实的影响及其与粒重的关系及内在机制。与常温处理相比, 幼穗分化期至抽穗期高温导致LYPJ和JLYHZ的千粒重、每穗颖花数、结实率和产量显著降低, 但对SY63无显著影响。幼穗分化期至抽穗期高温显著降低LYPJ和JLYHZ颖花和籽粒大小(长度、宽度和厚度), SY63的降幅低于热敏感品种。高温导致LYPJ颖花OsLOGL2基因表达降低、JLYHZ颖花OsCKX5基因表达增加, 并显著降低两品种颖花活性细胞分裂素含量。高温对2个热敏感品种成熟期地上部干物质积累无显著影响, 显著降低收获指数, 降低抽穗期至成熟期单粒增重和平均灌浆速率, 对SY63则无显著影响。高温显著降低了LYPJ和JLYHZ籽粒灌浆相关基因(OsFLO2、OsFLO4和OsGIF2)表达和籽粒酸性/中性转化酶、蔗糖合成酶、腺苷二磷酸葡萄糖焦磷酸化酶以及LYPJ籽粒淀粉分支酶活性, 对SY63无显著影响。本研究表明幼穗分化期至抽穗期高温降低了收获指数, 降低了颖花内源活性细胞分裂素含量从而降低颖花和籽粒大小, 也抑制了籽粒灌浆相关基因表达和相关酶活性; 因此, 高温导致粒重和产量下降可能是由于同化物向籽粒分配减少和库活性受到抑制, 表现出高温处理的后续效应。

关键词: 水稻, 幼穗分化期-抽穗期高温, 粒重, 籽粒形态, 籽粒充实, 颖花细胞分裂素含量

Abstract:

A pot experiment was conducted with two temperature treatments—normal temperature treatment and high temperature treatment during the panicle initiation and heading stages—using three rice varieties: heat-sensitive varieties Liangyoupeijiu (LYPJ) and Jingliangyouhuazhan (JLYHZ), and heat-tolerant variety Shanyou 63 (SY63). The objective was to investigate the effects of high temperature during the panicle initiation and heading stages on spikelet and grain size, grain filling, and their relationship with grain weight. Compared to normal temperature, high temperature significantly reduced thousand-grain weight, spikelets per panicle, seed setting rate, and yield in LYPJ and JLYHZ, but had no significant effect on SY63. High temperature also significantly decreased spikelet and grain size (length, width and thickness) in the heat-sensitive varieties, while SY63 exhibited only minor reductions. Additionally, high temperature significantly downregulated the expression of OsLOGL2 in spikelets of LYPJ and upregulated the expression of OsCKX5 in spikelets of JLYHZ, resulting in reduced endogenous active cytokinin levels in spikelets. High temperature decreased the harvest index but had no significant effect on aboveground dry weight at the maturity stage. It also significantly reduced single-grain weight accumulation and the average grain filling rate during the heading and maturity stages in the heat-sensitive varieties, with no significant changes observed in SY63. Furthermore, high temperature treatment significantly reduced the expression of grain filling-related genes (OsFLO2, OsFLO4 and OsGIF2) and the activities of key enzymes involved in grain filling, including acid/neutral invertases, sucrose synthetase, and ADP-glucose pyrophosphorylase, in the heat-sensitive varieties. Starch branching enzyme activity in grains was also significantly reduced in LYPJ, while these enzymes and genes were unaffected in SY63. This study demonstrates that high temperature during the panicle initiation and heading stages reduces the harvest index and decreases spikelet and grain size by lowering active cytokinin levels in spikelets. It also hinders grain filling by suppressing the expression of grain filling-related genes and enzyme activities. These findings suggest that the reductions in grain weight and yield under high temperature are likely due to decreased assimilate allocation to panicles and reduced sink activity, highlighting the adverse effects of heat stress on grain development and yield.

Key words: rice, high temperature, panicle initiation-heading stage, grain weight, grain shape, grain filling, content of cytokinins in spikelets

王梦宁, 谢可冉, 高逖, 王飞, 任孝俭, 熊栋梁, 黄见良, 彭少兵, 崔克辉. 水稻幼穗分化期至抽穗期高温对籽粒形态和充实的影响及其与粒重的关系[J]. 作物学报, 2025, 51(5): 1347-1362.

WANG Meng-Ning, XIE Ke-Ran, GAO Ti, WANG Fei, REN Xiao-Jian, XIONG Dong-Liang, HUANG Jian-Liang, PENG Shao-Bing, CUI Ke-Hui. Effect of high temperature during the panicle initiation and heading stages on grain shape and filling and its relationship with grain weight in rice[J]. Acta Agronomica Sinica, 2025, 51(5): 1347-1362.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2025.42046

https://zwxb.chinacrops.org/CN/Y2025/V51/I5/1347

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参考文献 52

[1]	IPCC. Climate Change 2021: The Physical Science Basis. Contribution of Working group I to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge, United Kingdom and New York, NY, USA: Cambridge University Press, 2021. p 3949.
[2]	Zhao C, Liu B, Piao S L, Wang X H, Lobell D B, Huang Y, Huang M T, Yao Y T, Bassu S, Ciais P, et al. Temperature increase reduces global yields of major crops in four independent estimates. Proc Natl Acad Sci USA, 2017, 114: 9326-9331. doi: 10.1073/pnas.1701762114 pmid: 28811375
[3]	Zhang C X, Feng B H, Chen T T, Zhang X F, Tao L X, Fu G F. Sugars, antioxidant enzymes and IAA mediate salicylic acid to prevent rice spikelet degeneration caused by heat stress. Plant Growth Regul, 2017, 83: 313-323.
[4]	Hu Q Q, Yan N, Cui K H, Li G H, Wang W C, Huang J L, Peng S B. Increased panicle nitrogen application improves rice yield by alleviating high-temperature damage during panicle initiation to anther development. Physiol Plant, 2024, 176: e14230.
[5]	Wu C, Cui K H, Wang W C, Li Q, Fahad S, Hu Q Q, Huang J L, Nie L X, Peng S B. Heat-induced phytohormone changes are associated with disrupted early reproductive development and reduced yield in rice. Sci Rep, 2016, 6: 34978. doi: 10.1038/srep34978 pmid: 27713528
[6]	刘建丰, 陈光辉, 何强, 李春庚. 不同产量水平杂交稻产量构成因素的分析. 云南农业大学学报, 2006, 21: 707-710.
	Liu J F, Chen G H, He Q, Li C G. A study on yield components of hybrid rice with big panicle. J Yunnan Agric Univ, 2006, 21: 707-710 (in Chinese with English abstract).
[7]	Liu L C, Tong H N, Xiao Y H, Che R H, Xu F, Hu B, Liang C Z, Chu J F, Li J Y, Chu C C. Activation of Big Grain1 significantly improves grain size by regulating auxin transport in rice. Proc Natl Acad Sci USA, 2015, 112: 11102-11107.
[8]	Ji X, Du Y X, Li F, Sun H Z, Zhang J, Li J Z, Peng T, Xin Z Y, Zhao Q Z. The basic helix-loop-helix transcription factor, OsPIL15, regulates grain size via directly targeting a purine permease gene OsPUP7 in rice. Plant Biotechnol J, 2019, 17: 1527-1537. doi: 10.1111/pbi.13075 pmid: 30628157
[9]	Shi C L, Dong N Q, Guo T, Ye W W, Shan J X, Lin H X. A quantitative trait locus GW6 controls rice grain size and yield through the gibberellin pathway. Plant J, 2020, 103: 1174-1188.
[10]	Li L F, Li J J, Liu K K, Jiang C L, Jin W H, Ye J K, Qin T R, Luo B J, Chen Z Y, Li J Z, et al. DGW1, encoding an hnRNP-like RNA binding protein, positively regulates grain size and weight by interacting with GW6 mRNA. Plant Biotechnol J, 2024, 22: 512-526.
[11]	She K C, Kusano H, Koizumi K, Yamakawa H, Hakata M, Imamura T, Fukuda M, Naito N, Tsurumaki Y, Yaeshima M, et al. A novel factor FLOURY ENDOSPERM2 is involved in regulation of rice grain size and starch quality. Plant Cell, 2010, 22: 3280-3294.
[12]	He Z S, Zeng J, Ren Y, Chen D, Li W J, Gao F Y, Cao Y, Luo T, Yuan G Q, Wu X H, et al. OsGIF1 positively regulates the sizes of stems, leaves, and grains in rice. Front Plant Sci, 2017, 8: 1730.
[13]	Wei X J, Jiao G A, Lin H Y, Sheng Z H, Shao G N, Xie L H, Tang S Q, Xu Q G, Hu P S. GRAIN INCOMPLETE FILLING 2 regulates grain filling and starch synthesis during rice caryopsis development. J Integr Plant Biol, 2017, 59: 134-153.
[14]	Lin L S, Qiu J J, Zhang L, Wei C X. Identification and analysis of nine new flo2 allelic mutants in rice. J Plant Physiol, 2024, 301: 154300.
[15]	Wang E T, Wang J J, Zhu X D, Hao W, Wang L Y, Li Q, Zhang L X, He W, Lu B R, Lin H X, et al. Control of rice grain-filling and yield by a gene with a potential signature of domestication. Nat Genet, 2008, 40: 1370-1374. doi: 10.1038/ng.220 pmid: 18820698
[16]	Huang L C, Tan H Y, Zhang C Q, Li Q F, Liu Q Q. Starch biosynthesis in cereal endosperms: an updated review over the last decade. Plant Commun, 2021, 2: 100237.
[17]	Morita S, Yonemaru J I, Takanashi J I. Grain growth and endosperm cell size under high night temperatures in rice (Oryza sativa L.). Ann Bot, 2005, 95: 695-701.
[18]	Wada H, Chang F Y, Hatakeyama Y, Erra-Balsells R, Araki T, Nakano H, Nonami H. Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice. Sci Rep, 2021, 11: 4447. doi: 10.1038/s41598-021-83870-1 pmid: 33627723
[19]	Shi W J, Yin X Y, Struik P C, Solis C, Xie F M, Schmidt R C, Huang M, Zou Y B, Ye C R, Krishna Jagadish S V. High day- and night-time temperatures affect grain growth dynamics in contrasting rice genotypes. J Exp Bot, 2017, 68: 5233-5245. doi: 10.1093/jxb/erx344 pmid: 29106621
[20]	Bahuguna R N, Solis C A, Shi W J, Jagadish K S V. Post-flowering night respiration and altered sink activity account for high night temperature-induced grain yield and quality loss in rice (Oryza sativa L.). Physiol Plant, 2017, 159: 59-73. doi: 10.1111/ppl.12485 pmid: 27513992
[21]	Zhang C X, Feng B H, Chen T T, Fu W M, Li H B, Li G Y, Jin Q Y, Tao L X, Fu G F. Heat stress-reduced kernel weight in rice at anthesis is associated with impaired source-sink relationship and sugars allocation. Environ Exp Bot, 2018, 155: 718-733.
[22]	董文军, 田云录, 张彬, 陈金, 张卫建. 非对称性增温对水稻品种南粳44米质及关键酶活性的影响. 作物学报, 2011, 37: 832-841. doi: 10.3724/SP.J.1006.2011.00832
	Dong W J, Tian Y L, Zhang B, Chen J, Zhang W J. Effects of asymmetric warming on grain quality and related key enzymes activities for japonica rice (Nanjing 44) under FATI facility. Acta Agron Sin, 2011, 37: 832-841 (in Chinese with English abstract).
[23]	Meng L, Wang C Y, Zhang J Q. Heat injury risk assessment for single-cropping rice in the middle and lower reaches of the Yangtze River under climate change. J Meteor Res, 2016, 30: 426-443.
[24]	吕川根, 宗寿余, 胡凝, 邹江石, 姚克敏, 唐卫亚. 两系杂交稻两优培九粒重因子的环境模型解析及生态特征分析. 作物学报, 2008, 34: 2202-2209. doi: 10.3724/SP.J.1006.2008.02202
	Lyu C G, Zong S Y, Hu N, Zou J S, Yao K M, Tang W Y. Modeling with climatic factors and analysis on ecological characters for grain weight dissected factors of two-line hybrid rice, Liangyou-peijiu. Acta Agron Sin, 2008, 34: 2202-2209 (in Chinese with English abstract).
[25]	Zhang X Z, Zhang Q P, Yang J, Jin Y H, Wu J S, Xu H, Xiao Y, Lai Y S, Guo Z Q, Wang J L, et al. Comparative effects of heat stress at booting and grain-filling stage on yield and grain quality of high-quality hybrid rice. Foods, 2023, 12: 4093.
[26]	Zhen F X, Zhou J J, Mahmood A, Wang W, Chang X N, Liu B, Liu L L, Cao W X, Zhu Y, Tang L. Quantifying the effects of short-term heat stress at booting stage on nonstructural carbohydrates remobilization in rice. Crop J, 2020, 8: 194-212. doi: 10.1016/j.cj.2019.07.002
[27]	Wu C, Cui K H, Li Q, Li L Y, Wang W C, Hu Q Q, Ding Y F, Li G H, Fahad S, Huang J L, et al. Estimating the yield stability of heat-tolerant rice genotypes under various heat conditions across reproductive stages: a 5-year case study. Sci Rep, 2021, 11: 13604. doi: 10.1038/s41598-021-93079-x pmid: 34193936
[28]	Yoshida S. Fundamentals of Rice Crop Science. Los Baños, Laguna, Philippines: the International Rice Research Institute, 1981.
[29]	徐建龙, 王俊敏, 骆荣挺, 张铭铣, 蒋兴村, 李金国. 空间诱变水稻大粒型突变体的遗传育种研究. 遗传, 2002, 24: 431-433.
	Xu J L, Wang J M, Luo R T, Zhang M X, Jiang X C, Li J G. Studies of inheritance and application in rice breeding of the large grain mutant induced in space environment. Hereditas (Beijing), 2002, 24: 431-433 (in Chinese with English abstract).
[30]	魏颖娟, 赵杨, 邹应斌. 不同穗型超级稻品种籽粒灌浆特性. 作物学报, 2016, 42: 1516-1529. doi: 10.3724/SP.J.1006.2016.01516
	Wei Y J, Zhao Y, Zou Y B. Grain-filling characteristics in super rice with different panicle types. Acta Agron Sin, 2016, 42: 1516-1529 (in Chinese with English abstract).
[31]	韦还和, 张翔, 朱旺, 耿孝宇, 马唯一, 左博源, 孟天瑶, 高平磊, 陈英龙, 许轲, 等. 盐胁迫对水稻籽粒灌浆特性及产量形成的影响. 作物学报, 2024, 50: 734-746. doi: 10.3724/SP.J.1006.2024.32021
	Wei H H, Zhang X, Zhu W, Geng X Y, Ma W Y, Zuo B Y, Meng T Y, Gao P L, Chen Y L, Xu K, et al. Effects of salinity stress on grain-filling characteristics and yield of rice. Acta Agron Sin, 2024, 50: 734-746 (in Chinese with English abstract).
[32]	Li G H, Pan J F, Cui K H, Yuan M S, Hu Q Q, Wang W C, Mohapatra P K, Nie L X, Huang J L, Peng S B. Limitation of unloading in the developing grains is a possible cause responsible for low stem non-structural carbohydrate translocation and poor grain yield formation in rice through verification of recombinant inbred lines. Front Plant Sci, 2017, 8: 1369. doi: 10.3389/fpls.2017.01369 pmid: 28848573
[33]	Liu L, Cui K H, Qi X L, Wu Y, Huang J L, Peng S B. Varietal responses of root characteristics to low nitrogen application explain the differing nitrogen uptake and grain yield in two rice varieties. Front Plant Sci, 2023, 14: 1244281.
[34]	Xu C S, Yang F, Tang X N, Lu B, Li Z Y, Liu Z H, Ding Y F, Ding C, Li G H. Super rice with high sink activities has superior adaptability to low filling stage temperature. Front Plant Sci, 2021, 12: 729021.
[35]	Zheng C K, Zhou G H, Zhang Z Z, Li W, Peng Y B, Xie X Z. Moderate salinity stress reduces rice grain yield by influencing expression of grain number- and grain filling-associated genes. J Plant Growth Regul, 2021, 40: 1111-1120.
[36]	Chen Y H, Wang Y L, Chen H Z, Xiang J, Zhang Y K, Wang Z G, Zhu D F, Zhang Y P. Brassinosteroids mediate endogenous phytohormone metabolism to alleviate high temperature injury at panicle initiation stage in rice. Rice Sci, 2023, 30: 70-86.
[37]	Ji D L, Xiao W H, Sun Z W, Liu L J, Gu J F, Zhang H, Harrison M T, Liu K, Wang Z Q, Wang W L, et al. Translocation and distribution of carbon-nitrogen in relation to rice yield and grain quality as affected by high temperature at early panicle initiation stage. Rice Sci, 2023, 30: 598-612.
[38]	杨连新, 王余龙, 董桂春, 张亚洁, 单玉华, 杨洪建. 栽培和环境条件对水稻饱粒重的影响. 江苏农业科学, 2002, 30(6): 9-13.
	Yang L X, Wang Y L, Dong G C, Zhang Y J, Shan Y H, Yang H J. Effects of different cultural and environmental conditions on fulfilled grain weight in rice. Jiangsu Agric Sci, 2002, 30(6): 9-13 (in Chinese).
[39]	Zhen F X, Wang W, Wang H Y, Zhou J J, Liu B, Zhu Y, Liu L L, Cao W X, Tang L. Effects of short-term heat stress at booting stage on rice-grain quality. Crop Past Sci, 2019, 70: 486.
[40]	曹云英, 段骅, 杨立年, 王志琴, 周少川, 杨建昌. 减数分裂期高温胁迫对耐热性不同水稻品种产量的影响及其生理原因. 作物学报, 2008, 34: 2134-2142. doi: 10.3724/SP.J.1006.2008.02134
	Cao Y Y, Duan H, Yang L N, Wang Z Q, Zhou S C, Yang J C. Effect of heat-stress during meiosis on grain yield of rice cultivars differing in heat-tolerance and its physiological mechanism. Acta Agron Sin, 2008, 34: 2134-2142 (in Chinese with English abstract).
[41]	Schmülling T, Werner T, Riefler M, Krupková E, Manns I B Y. Structure and function of cytokinin oxidase/dehydrogenase genes of maize, rice, Arabidopsis and other species. J Plant Res, 2003, 116: 241-252. doi: 10.1007/s10265-003-0096-4 pmid: 12721786
[42]	Kurakawa T, Ueda N, Maekawa M, Kobayashi K, Kojima M, Nagato Y, Sakakibara H, Kyozuka J. Direct control of shoot meristem activity by a cytokinin-activating enzyme. Nature, 2007, 445: 652-655.
[43]	陈燕华, 王亚梁, 朱德峰, 石庆华, 陈惠哲, 向镜, 张义凯, 张玉屏. 外源油菜素内酯缓解水稻穗分化期高温伤害的机理研究. 中国水稻科学, 2019, 33: 457-466. doi: 10.16819/j.1001-7216.2019.9036
	Chen Y H, Wang Y L, Zhu D F, Shi Q H, Chen H Z, Xiang J, Zhang Y K, Zhang Y P. Mechanism of exogenous brassinolide in alleviating high temperature injury at panicle initiation stage in rice. Chin J Rice Sci, 2019, 33: 457-466 (in Chinese with English abstract). doi: 10.16819/j.1001-7216.2019.9036
[44]	吴超. 生殖生长期高温对水稻产量形成的影响及其激素调控机理研究. 华中农业大学博士学位论文, 湖北武汉, 2016.
	Wu C. Effects of High Temperature During the Reproductive Stages on Rice Yield Formation and Its Phytohormonal Basis. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2016 (in Chinese with English abstract).
[45]	Jiang N, Yu P H, Fu W M, Li G Y, Feng B H, Chen T T, Li H B, Tao L X, Fu G F. Acid invertase confers heat tolerance in rice plants by maintaining energy homoeostasis of spikelets. Plant Cell Environ, 2020, 43: 1273-1287.
[46]	Chen Y H, Chen H Z, Xiang J, Zhang Y K, Wang Z G, Zhu D F, Wang J K, Zhang Y P, Wang Y L. Rice spikelet formation inhibition caused by decreased sugar utilization under high temperature is associated with brassinolide decomposition. Environ Exp Bot, 2021, 190: 104585.
[47]	Lin G Q, Yang Y, Chen X Y, Yu X R, Wu Y F, Xiong F. Effects of high temperature during two growth stages on caryopsis development and physicochemical properties of starch in rice. Int J Biol Macromol, 2020, 145: 301-310. doi: S0141-8130(19)35966-5 pmid: 31874272
[48]	胡秋倩. 氮供应对水稻幼穗分化期高温下产量形成的影响及机理研究. 华中农业大学博士学位论文, 湖北武汉, 2021.
	Hu Q Q. Effects of Different Nitrogen Rates on Yield Formation under High Temperature during Panicle Initiation Stage and Its Mechanism in Rice. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2021 (in Chinese with English abstract).
[49]	Wang Y L, Zhang Y K, Shi Q H, Chen H Z, Xiang J, Hu G H, Chen Y H, Wang X D, Wang J K, Yi Z H, et al. Decrement of sugar consumption in rice young panicle under high temperature aggravates spikelet number reduction. Rice Sci, 2020, 27: 44-55. doi: 10.1016/j.rsci.2019.12.005
[50]	张玉屏, 王军可, 王亚梁, 陈燕华, 朱德峰, 陈惠哲, 向镜, 张义凯, 刘小军, 朱艳, 等. 水稻淀粉合成对夜温变化的响应. 中国水稻科学, 2020, 34: 525-538. doi: 10.16819/j.1001-7216.2020.0701
	Zhang Y P, Wang J K, Wang Y L, Chen Y H, Zhu D F, Chen H Z, Xiang J, Zhang Y K, Liu X J, Zhu Y, et al. Response of rice starch synthesis to night temperature changes. Chin J Rice Sci, 2020, 34: 525-538 (in Chinese with English abstract).
[51]	赵步洪, 张洪熙, 朱庆森, 杨建昌. 两系杂交稻籽粒充实不良的成因及其与激素含量的关系. 中国农业科学, 2006, 39: 477-486.
	Zhao B H, Zhang H X, Zhu Q S, Yang J C. Causes of poor grain plumpness of two-line hybrids and their relationships to contents of hormones in the rice grain. Sci Agric Sin, 2006, 39: 477-486 (in Chinese with English abstract). doi: 10.3864/j.issn.0578-1752.at-2005-6166
[52]	刘晓龙, 叶世河, 廖俊婕, 骆依菲, 龙莎, 廖婧芃, 钟歆, 谢菲, 谢子怡, 曾鹏, 等. 灌浆初期高温胁迫对水稻籽粒活性氧积累及产量形成的影响. 西北农林科技大学学报(自然科学版), 2024, 52(5): 33-47.
	Liu X L, Ye S H, Liao J J, Luo Y F, Long S, Liao J P, Zhong X, Xie F, Xie Z Y, Zeng P, et al. Effect of heat stress during early filling stage on ROS accumulation and yield formation in rice grain. J Northwest A&F Univ (Nat Sci Edn), 2024, 52(5): 33-47 (in Chinese with English abstract).

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基因登录号 Accession number (NCBI)	基因 Gene	正向引物 Forward primer (5'-3')	反向引物 Reverse primer (5'-3')
XM015767376	OsLOGL2	GAGCGCACAGAAAAGAGAAGC	GGCATGAGTGCTTTTGGAAT
NM001423909	OsLOGL3	GTGCTGCATTGTCTGCAGTT	GGTCATGAGAGTCTTGGGGA
NM001401734	OsCKX5	CGCTGCTGGGCGAGCTGAAT	CGCCTTGTGCACGCGGTCTA
NM001420459	OsCKX9	GCCAGGATTCCTCTTGAACCTGC	ACGCACTGGGTCCTGCGGAT
NM001402366	OsGIF1	TTCTCAAGGACAGGGTGGTCAAGC	TCAGCCTGTGCAGTTTGTAGCC
XM015794875	OsGIF2	AAGAGGTGCTTTGGTGATG	GTCCGAAGATGAGGAGTGT
XM015780251	OsFLO2	CACACCCTCCAGCAATATCA	CCTTCTGCGACTGCTTTTCT
XM015795182	OsFLO4	CATGCACTGTTCGAGGAGAA	GGGAAATGGCTCTCCCTTAG
NM001418593	Actin	CAATCGTGAGAAGATGACCC	GTCCATCAGGAAGCTCGTAGC

品种 Variety	处理 Treatment	产量 Yield (g plant^-1)	有效穗数 Panicles (No. plant^-1)	每穗颖花数 Spikelets (No. panicle^-1)	结实率 Seed- setting percentage (%)	千粒重 1000-grain weight (g)	抽穗后10 d地上部干重 Aboveground dry weight at 10 days after heading (g plant^-1)	成熟期地上部干重 Aboveground dry weight at maturity stage (g plant^-1)	收获指数 Harvest index (%)
LYPJ	CK	25.7±0.7 b	9.4±0.1 b	141.0±0.9 b	85.2±2.2 a	22.9±0.1 b	48.3±4.7 ab	81.3±1.5 ab	31.6±0.5 b
	HT	6.7±1.5 c^*	8.7±0.2 b^*	122.3±5.6 c^*	30.8±5.4 d^*	20.0±0.1 c^*	44.9±1.8 b	69.2±4.5 b	9.6±1.7 e^*
	下降幅度 Falling range (%)	73.9	7.4	13.3	63.8	12.7	7.0	14.9	69.6
JLYHZ	CK	32.0±0.5 a	11.8±0.3 a	173.7±8.3 a	83.1±2.1 a	18.9±0.2 d	41.3±1.7 b	79.9±0.9 ab	40.1±0.8 a
	HT	10.8±1.1 c^*	11.6±0.1 a	130.6±0.3 bc^*	45.7±4.5 c^*	15.7±0.2 e^*	41.1±4.2 b	65.4±1.3 b^*	16.6±1.7 d^*
	下降幅度 Falling range (%)	66.3	1.7	24.8	45.0	16.9	0.5	18.1	58.6
SY63	CK	28.0±1.7 ab	8.8±0.8 b	169.2±3.4 a	77.2±1.1 ab	24.7±0.4 a	54.8±4.1 a	91.1±4.1 a	30.4±0.8 bc
	HT	24.3±2.3 b	8.7±1.0 b	169.3±10.2 a	68.9±3.1 b	24.6±0.2 a	55.0±1.5 a	92.5±12.2 a	27.4±0.6 c^*
	下降幅度 Falling range (%)	13.2	1.0	-0.1	10.8	0.4	-0.4	-1.5	9.9

品种 Variety	处理 Treatment	颖花Spikelet		籽粒Grain
品种 Variety	处理 Treatment	长度 Length (mm)	宽度 Width (mm)	长度 Length (mm)	宽度 Width (mm)	厚度 Thickness (mm)	体积 Volume (mm³)
LYPJ	CK	9.48±0.06 a	2.84±0.01 c	9.05±0.01 a	2.66±0.00 c	1.88±0.01 b	45.11±0.34 c
	HT	8.00±0.21 d^*	2.37±0.09 e^*	8.58±0.06 c^*	2.44±0.00 e^*	1.68±0.01 d^*	35.09±0.39 e^*
	下降幅度 Falling range (%)	15.6	16.5	5.2	8.3	10.6	22.2
JLYHZ	CK	8.73±0.01 b	2.71±0.00 d	8.73±0.03 b	2.59±0.01 d	1.81±0.00 c	41.06±0.08 d
	HT	7.98±0.05 d^*	2.36±0.02 e^*	8.58±0.03 c^*	2.40±0.01 f^*	1.65±0.00 e^*	34.01±0.26 f^*
	下降幅度 Falling range (%)	8.6	12.9	1.7	7.3	8.8	17.2
SY63	CK	8.30±0.03 c	3.26±0.01 a	8.38±0.02 d	3.13±0.01 a	1.90±0.01 a	49.96±0.19 a
	HT	8.36±0.06 c	3.04±0.02 b^*	8.27±0.03 d^*	2.98±0.01 b^*	1.89±0.01 ab	46.51±0.18 b^*
	下降幅度 Falling range (%)	-0.7	6.7	1.3	4.8	0.5	6.9

品种 Variety	温度处理 Temperature treatment	aCTK (ng g^-1)	tZ+tZR (ng g^-1)	iP+iPA+iPMP (ng g^-1)	IAA (ng g^-1)	GA₁ (ng g^-1)
LYPJ	CK	24.53±1.81 a	7.10±0.11 a	17.43±1.73 a	3.34±0.22 c	5.50±0.31 a
	HT	20.32±0.66 b	6.88±0.50 a	13.44±0.21 b	3.18±0.12 c	4.68±0.95 a
	下降幅度 Falling range (%)	17.2	3.1	22.9	4.8	14.9
JLYHZ	CK	25.61±1.09 a	6.41±0.10 a	19.20±1.11 a	5.28±0.11 a	4.20±0.27 a
	HT	20.16±0.81 b^*	6.61±0.51 a	13.55±0.34 b^*	5.41±0.11 a	3.10±0.51 a
	下降幅度 Falling range (%)	21.3	-3.1	29.4	-2.5	26.2
SY63	CK	22.78±0.05 ab	6.55±0.12 a	16.23±0.09 ab	4.72±0.09 b	3.15±0.43 a
	HT	23.45±0.96 ab	7.27±0.24 a	16.18±0.89 ab	4.90±0.09 b	5.27±1.54 a
	下降幅度 Falling range (%)	-2.9	-10.1	0.3	-3.8	-67.3

水稻幼穗分化期至抽穗期高温对籽粒形态和充实的影响及其与粒重的关系

Effect of high temperature during the panicle initiation and heading stages on grain shape and filling and its relationship with grain weight in rice

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