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作物学报 ›› 2017, Vol. 43 ›› Issue (01): 9-18.doi: 10.3724/SP.J.1006.2017.00009

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦茎秆实心度对茎秆强度的影响及相关性状QTL分析

潘婷1,**,胡文静2,**,李东升2,程晓明2,吴荣林2,程顺和1,2,*   

  1. 南京农业大学作物遗传与种质创新国家重点实验室，江苏南京210095；2江苏里下河地区农业科学研究所 / 国家小麦改良中心扬州分中心，江苏扬州225007
  • 收稿日期:2016-03-10 修回日期:2016-09-18 出版日期:2017-01-12 网络出版日期:2016-10-09
  • 通讯作者: 程顺和, E-mail:13905273926@126.com**同等贡献(Contributed equally to this work)
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-03-03B, CARS-3-2-11)和江苏省农业科技自主创新资金项目[CX(13)2022]资助。

Influence of Stem Solidness on Stem Strength and Stem Solidness Associated QTLs in Bread Wheat

PAN Ting1,**,HU Wen-Jing2,**,LI Dong-Sheng2,CHENG Xiao-Ming2,WU Rong-Lin2,CHENG Shun-He1,2,*   

  1. 1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Agriculture Sciences of the Lixiahe District / Branch of National Wheat Improvement Center, Yangzhou 225007, China
  • Received:2016-03-10 Revised:2016-09-18 Published:2017-01-12 Published online:2016-10-09
  • Contact: 程顺和, E-mail:13905273926@126.com**同等贡献(Contributed equally to this work)
  • Supported by:

    This study was supported by the China Agriculture Research System(CARS-03-03B, CARS-3-2-11) and theIndependent Innovation Fundfor Agricultural Science and Technology in Jiangsu Province [CX(13)2022].

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摘要：

在QTL水平上研究茎秆实心度与强度的遗传关系及实心度对茎秆强度的影响，为小麦抗倒伏育种提供依据。利用普通小麦宁麦18 与实秆小麦种质“武云实秆”的F2群体和F2:3家系，对小麦茎秆强度、实心度及影响实心度的相关性状包括厚径比、壁厚、茎粗和髓腔直径进行了相关分析，并对茎秆强度相关性状QTL进行分子标记定位及遗传效应分析。结果表明小麦茎秆强度与厚径比、壁厚均呈极显著正相关，与髓腔直径呈极显著负相关。基于复合区间作图法进行QTL定位，检测到与茎秆强度、厚径比、壁厚、茎粗和髓腔直径相关QTL共23个，分布在1B、3B、4A、4B、5A上，表型贡献率3.5%~44.0%。在染色体3B、4A和5A上的标记区间gwm547–gwm247、wmc718–wmc468和gwm156–gwm443均检测到贡献率很高的茎秆实心度相关QTL，说明在这3条染色体上可能存在控制茎秆强度的主效QTL。用普通小麦宁麦13(N13)×武云实秆的24个F7家系检验分子标记gwm247的可靠性表明利用标记gwm247选育茎秆实心度优于宁麦13的概率较大。研究结果为进一步精细定位相关主效QTL以及分子标记辅助改良小麦茎秆强度奠定了基础。

关键词: 小麦, 茎秆强度, 实心度, QTL定位

Abstract:

Improving stem strength is an important way to increase lodging resistance of wheat. Solid stem traits, including thickness-diameter ratio of culm, stem diameter, culm wall thickness and pith diameter, are the main targets for improvement of stem strength in wheat. In the present study, an F2 population and F2:3 families derived from the cross N18×WYSG wereused to study the influence of stem solidness on stem strength and to identify QTLs for solidnessrelated traits. The thickness-diameter ratio of culm, stem diameter, culm wall thickness and pith diameter were significantly correlated with stem strength. By using composite interval mapping (CIM), a total of 23 QTLs for the five traitswere detected on chromosomes 1B, 3B, 4A, 4B and 5A. Phenotypic variations explained by individual QTL ranged from 3.5% to 44.0%. Pleiotropic QTLs were found on chromosomes 3B (gwm547–gwm247),4A (wmc718–wmc468) and 5A (gwm156–gwm443), all contributing very high phenotypic variations. The reliability of the marker gwm247was confirmed using 24 F7 lines derived from the N13 (hollow stem) × WYSG (solid stem) cross. The markers linked to major QTLs could be valuable in wheat breeding for lodging resistance with marker assisted selection technology.

Key words: Wheat, Stem strength, Stem solidness, Quantitative trait loci

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