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作物学报 ›› 2010, Vol. 36 ›› Issue (05): 754-763.doi: 10.3724/SP.J.1006.2010.00754

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

太湖流域粳稻两类群体种子活力性状有利等位变异的发掘

王洋1,2,郭媛1,3,洪德林1,*   

  1. 1南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095;2黑龙江大学农业资源与环境学院,黑龙江哈尔滨150008;3中国农业科学院麻类研究所,湖南长沙410205
  • 收稿日期:2009-08-22 修回日期:2009-12-19 出版日期:2010-05-12 网络出版日期:2010-03-03
  • 通讯作者: 洪德林, E-mail: delinhong@njau.edu.cn
  • 基金资助:

    本研究由高等学校学科创新引智计划项目(B08025)和教育部科技基础条件平台重点项目(505005)资助.

Mining Elite Alleles for Seed Vigor Traits in Two Populations of Japonica Rice (Oryza sativa L.) in Taihu Lake Region

WANG Yang1,2,GUO Yuan1,3,HONG De-Lin1,*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Department of Agricultural Resource and Environment, Heilongjiang University, Harbin 150008, China; 3 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
  • Received:2009-08-22 Revised:2009-12-19 Published:2010-05-12 Published online:2010-03-03
  • Contact: HONG De-Lin,E-mail:delinhong@njau.edu.cn

摘要:

发掘粳稻种子活力性状的优异等位变异和携带优异等位变异的载体材料可为培育适于直播的高活力粳稻品种提供遗传信息和育种材料。以太湖流域粳稻94个品种构成的自然群体和粳稻品种秀水79与粳稻恢复系C堡衍生的247个重组自交家系(RIL)群体为试验材料,利用斜板发芽法发芽,调查生长7 d的幼苗根长、苗高和干重3个种子活力性状,采用Tassel软件中的GLM方法和Win QTL Cartographer 2.5软件中的CIM方法进行种子活力性状的QTL分析,发掘有利等位变异和相应载体品种。结果表明:(1) 在太湖流域粳稻自然群体中共检测到11个与种子活力性状相关联的SSR标记位点,共发掘出42个控制种子活力性状的优异等位变异,其中控制根长的17个,控制苗高的13个,控制幼苗干重的12个。携带种子活力性状优异等位变异且效应值较大的载体材料有滇屯502选早、扬稻6号、开青、籼恢429和C堡等。(2) 在RIL群体中共检测到9个与种子活力性状相关的QTL,其中2个控制根长,4个控制苗高,3个控制幼苗干重。除控制幼苗干重的qDW-2a的有利等位变异RM525-143 bp来自秀水79以外,其余8个位点的有利等位变异均来自C堡。(3) 两类群体均在第1染色体上检测到与根长关联的SSR标记位点,均在第2、第8和第11染色体上检测到与苗高关联的位点,均在第2染色体上检测到与幼苗干重关联的位点,且在自然群体中检测到优于和多于家系群体的等位变异。

关键词: 粳稻(Oryza sativa L.), 种子活力, 有利等位变异, 自然群体, 家系群体

Abstract:

Direct seeding in rice (Oryza sativa L.) is a labor-saving, high efficient and water-saving cultivation pattern. Lower temperature stress is the main factor affecting seedling establishment in direct seeding rice field. Breeding rice cultivars with high seed vigor is one of the ways to improve seedling establishment. In order to provide genetic information and breeding materials for breeding cultivars suitable to direct sowing in japonica rice, we conducted this research. Root length, shoot height, and dry weight of seedlings germinated for 7 days on the sloped board were investigated in a natural population composed of 94 varieties from Taihu Lake Region and a recombinant inbred line (RIL) population derived from a cross between Xiushui 79 and C Bao in japonica rice (Oryza sativa L.). QTL analysis of seed vigor was performed by using General Linear Model program in Tassel software for the natural population and Composite Internal Mapping program in Win QTL Cartographer 2.5 software for the RIL population. Elite alleles and their carrier materials were determined on the basis of the result of QTL analysis. The results obtained were as follows: (1) In the natural population, eleven SSR marker loci associated with seed vigor were detected. Forty-two elite alleles for seed vigor traits were detected in the natural population. Among them, 17 associated with root length, 13 with shoot height and 12 with dry weight. Diantun 502 xuanzao, Yangdao 6, Kaiqing, Xianhui 429 and C Bao were carrier materials for the elite alleles of seed vigor. (2) In the RIL population, nine QTLs, including two for root length, four for shoot height and three for dry weight, were detected. All the elite alleles came from C Bao except RM525-143 bp, which associated with dry weight and came from Xiushui 79. (3) In both populations, SSR marker locus associated with root length was detected on chromosome 1, SSR marker loci associated with shoot height were detected on chromosomes 2, 8 and 11, and SSR marker locus associated with dry weight was detected on chromosome 2. More or better elite alleles were detected in the natural population than in the RIL population.

Key words: Japonica rice(Oryza sativa L.), Seed vigor, Elite alleles, Natural population, Family population

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