作物学报 ›› 2011, Vol. 37 ›› Issue (11): 1991-2000.doi: 10.3724/SP.J.1006.2011.01991
张长伟1,2,凌英华1,桑贤春1,李平1,赵芳明1,杨正林1,李云峰1,方立魁1,何光华1,*
ZHANG Chang-Wei1,2,LING Ying-Hua1,SANG Xian-Chun1,LI Ping1,ZHAO Fang-Ming1,YANG Zheng-Lin1,LI Yun-Feng1,FANG Li-Kui1,HE Guang-Hua1,*
摘要: 稻瘟病是危害水稻的最严重病害之一,探索利用外源基因提高水稻抗稻瘟病水平对现代育种具有重要意义。本研究从T2代起逐代增加抗性选择压,采用苗期人工混合接种和病圃田自然诱发等鉴定方法,对转基因水稻后代进行稻瘟病抗性鉴定和筛选,在T5代获得了7个稻瘟病抗性极显著增强的转McCHIT1基因水稻稳定株系。通过7个生理群26个生理小种的115个稻瘟病有效单孢菌株苗期抗谱测定,这7个株系的抗病频率为52.2%~61.4%,比受体对照缙恢35 (36.8%)高16个百分点以上,主要增加了对ZE群生理小种的抗性,提高了对ZG群、ZF群和优势种群ZB群生理小种的抗性。C36-2-1、C21-6-2、C21-3-1等3个株系的结实率达80%以上,是丰抗结合较好的转McCHIT1基因株系。McCHIT1基因具有广谱抗性,将其遗传转化水稻,采用“逐代增加选择压,抗中选抗”的方法,可筛选出稻瘟病抗性优良、抗谱明显拓宽、产量性状较好的转基因稳定株系。
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