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作物学报 ›› 2020, Vol. 46 ›› Issue (12): 1894-1904.doi: 10.3724/SP.J.1006.2020.04050

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

外源茉莉酸甲酯对UV-B胁迫下颠茄生物碱积累及TAs代谢途径调控的机制探究

山雨思1,2(), 辛正琦1,2, 何潇1,2, 代欢欢1,2, 吴能表1,2,*()   

  1. 1西南大学生命科学学院, 重庆 400715
    2三峡库区生态环境教育部重点实验室, 重庆 400715
  • 收稿日期:2020-02-28 接受日期:2020-06-02 出版日期:2020-12-12 网络出版日期:2020-07-28
  • 通讯作者: 吴能表
  • 基金资助:
    国家自然科学基金项目(30500041)

Mechanism exploration on alkaloids accumulation and TAs metabolic pathways regulation in Atropa belladonna L. treated with exogenous methyl jasmonate under UV-B stress

Yu-Si SHAN1,2(), Zheng-Qi XIN1,2, Xiao HE1,2, Huan-Huan DAI1,2, Neng-Biao WU1,2,*()   

  1. 1School of Life Science, Southwest University, Chongqing 400715, China
    2Key Laboratory of Eco-environments in Three Gorges Region, Ministry of Education, Chongqing 400715, China
  • Received:2020-02-28 Accepted:2020-06-02 Published:2020-12-12 Published online:2020-07-28
  • Contact: Neng-Biao WU
  • Supported by:
    National Natural Science Foundation of China(30500041)

摘要:

以颠茄(Atropa belladonna L.)为材料, 采用外源茉莉酸甲酯(methyl jasmonate, MeJA)处理颠茄幼苗, 研究UV-B胁迫下不同浓度外源MeJA在不同处理时间对颠茄托品烷类生物碱(tropane alkaloids, TAs)含量、TAs合成途径上游产物、信号分子以及关键酶基因表达量的影响, 初步探究UV-B胁迫下外源MeJA调控颠茄TAs代谢途径的机制。UV-B胁迫显著降低了颠茄莨菪碱和东莨菪碱的含量, 对TAs合成途径前体物质的积累产生抑制作用, 不利于TAs合成代谢。经适宜浓度的MeJA处理后, 颠茄TAs含量有一定程度的提高, 次生代谢途径中前体氨基酸(鸟氨酸和精氨酸)、多胺含量以及腐胺合成关键酶活性也均有不同程度的上升; 信号分子NO的浓度随MeJA浓度的升高呈先升后降的趋势, 在MeJA浓度为250 μmol L -1时达到最高。对TAs合成途径关键酶基因表达量的检测发现, 在外源MeJA的诱导下, 颠茄叶片和根部TR IPPARH6H表达量均有不同程度的上调。表明外源MeJA通过诱导颠茄TAs合成上游产物含量的升高, 刺激NO的迸发, 为TAs合成途径提供更多的前体物质, 同时通过调控TAs代谢途径中关键酶基因TR IPPARH6H的高效表达, 有效缓解UV-B胁迫对颠茄TAs的抑制作用, 提高颠茄莨菪碱和东莨菪碱的含量。以上研究结果可为今后进一步研究逆境胁迫下外源诱导子调控颠茄TAs次生代谢途径机制提供依据, 以期在实际生产中有效提高颠茄的抗逆性和其药用成分的积累。

关键词: 茉莉酸甲酯, UV-B胁迫, 颠茄, 托品烷类生物碱, 次生代谢

Abstract:

In order to preliminarily explore the mechanism of TAs metabolic pathway in A. belladonna treated with exogenous methyl jasmone under UV-B stress, the effects on the contents of hyoscyamine and scopolamine, the upstream products in alkaloid synthesis, signal molecule and the expression level of key enzyme genes of secondary metabolism were studied under different concentrations of exogenous MeJA and different treatment time under using A. belladonna as materials. UV-B stress treatment significantly reduced the contents of hyoscyamine and scopolamine, inhibited the accumulation of precursors in the TAs synthesis pathway, which harmful to TAs synthesis. The content of TAs in A. belladonna increased to some extent, the contents of precursor amino acids (ornithine, arginine), polyamine content and key enzymes activities in the synthesis of putrescine in the secondary metabolic pathway all increased to some extent after treatment with the appropriate concentration of MeJA. The concentrations of signal molecule NO firstly increased and then decreased with the rising MeJA concentration, and reached the highest when MeJA concentration was 250 μmol L -1. The expression of key enzyme genes in TAs synthesis pathway showed that exogenous MeJA could increase the relative gene expression levels of TR I, PPAR, H6H to some extent. Those indicated that exogenous MeJA could induce the increase in the contents of upstream products in TAs synthesis by stimulating the burst of NO resulted in more precursor materials for the TAs synthesis pathway and affect the high expression of TR I, PPAR and H6H. It alleviated the inhibiton of UV-B stress on TAs of A. belladonna and increased the contents of hyoscyamine and scopolamine effectively. The results provided a theoretical basis for further studying the mechanism of exogenous elicitors to regulate the TAs secondary metabolic pathway of A. belladonn under stress, and effectively improved the stress resistance of A. belladonn and the accumulation of medicinal ingredients in actual production.

Key words: methyl jasmonate, UV-B stress, Atropa belladonna L., tropane alkaloids, secondary metabolism

表1

荧光定量PCR所需引物"

基因
Gene
上游引物
Forward primer (5′-3′)
下游引物
Reverse primer (5′-3′)
PGK TCGCTCTTGGAGAAGGTTGAC CTTGTCCGCAATCACTACATCAG
PMT CCTACTTACCCTACTGGTGTTATC GCGAAAGATGGCAAAATAAAAGC
TR I TTCTTTGCTTCCCTGCTGCTTC CAGGCCAACCTTAGTATCACACAG
PPAR GATTGGAGTTGTTGGATTGG CCTTGAAGTGTAAGAGATGGA
H6H TTCCACTTGAGCAGAAAGCAAAGC CCTCATGGTCAACTTCCTCACTTCC

表2

不同浓度外源MeJA对UV-B胁迫下颠茄叶片莨菪碱和东莨菪碱含量的影响"

指标
Indicator
处理组
Treatment group
处理时间Treatment time
4 d 8 d 12 d 16 d
莨菪碱
Hyoscyamine
CK1 477.988±30.032 d 464.993±39.364 a 510.286±29.327 a 489.386±33.957 a
CK2 514.323±36.595 c 403.706±14.900 c 350.179±35.316 d 295.220±30.438 d
T1 527.659±38.739 bc 423.530±36.157 bc 375.603±19.982 c 312.172±19.933 cd
T2 542.565±35.559 bc 468.808±27.539 a 392.611±37.487 bc 361.054±22.242 bc
T3 581.520±17.200 a 447.993±24.571 b 425.491±47.568 b 384.890±44.869 b
T4 564.200±33.989 ab 430.305±15.919 bc 369.667±40.042 c 332.059±26.077 c
东莨菪碱
Scopolamine
CK1 267.673±24.260 a 261.248±22.428 a 294.230±42.998 a 282.621±28.477 a
CK2 221.892±20.219 c 198.795±34.491 c 174.729±14.562 d 121.804±15.205 d
T1 243.889±28.790 ab 217.900±17.821 bc 193.653±21.253 cd 130.244±24.996 cd
T2 237.625±28.217 ab 236.775±13.461 ab 221.873±19.211 b 150.869±26.035 c
T3 248.261±20.375 ab 234.134±26.418 b 229.996±29.857 b 185.867±23.527 b
T4 225.705±14.739 bc 220.173±26.487 bc 208.852±24.722 bc 164.412±30.129 c

图1

不同浓度外源MeJA对UV-B胁迫下颠茄叶片鸟氨酸和精氨酸含量的影响 标以不同小写字母表示不同处理组间差异显著(P < 0.05)。处理同表2。"

图2

不同浓度外源MeJA对UV-B胁迫下颠茄叶片ODC和ADC酶活性的影响 标以不同小写字母表示不同处理组间差异显著(P < 0.05)。处理同表2。"

图3

不同浓度外源MeJA对UV-B胁迫下颠茄叶片多胺含量(A), 多胺总量(B)和(Spm+Spd)/Put比值(C)的影响 标以不同小写字母表示不同处理组间差异显著(P < 0.05)。处理同表2。"

图4

不同浓度外源MeJA对UV-B胁迫下颠茄叶片NO浓度的影响 标以不同小写字母表示不同处理组间差异显著(P < 0.05)。处理同表2。"

图5

不同浓度外源MeJA对UV-B胁迫下颠茄叶片(A)、根(B)中PMT、TR I、PPAR、H6H基因表达量的影响 标以不同小写字母表示不同处理组间差异显著(P < 0.05)。处理同表2。"

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