关键词: WAPO1, 每穗小穗数, 优异单倍型, 地理分布, 育种利用

Abstract:

Wheat is one of the most important crops, and spike is an important trait affecting yield. WAPO1, encoding an F-box protein, is an ortholog of rice APO1 in wheat. In this study, superior haplotypes of WAPO1 were identified and their genetic effects, geographical distribution, and breeding utilization were further analyzed. The SNP (G/T) in F-box region was identified by using the developed KASP marker and 115 bp insertion/deletion of promoter was identified to analyze the haplotypes by the reported InDel marker in Chinese wheat landraces. In addition to the previously identified three haplotypes (H1: 140^G+115^deletion, H2: 140^T+115^insertion, and H3: 140^G+115^insertion), we identified a new and fourth haplotype (H4: 140^T+115^deletion) and proved that H2 and H4 were superior haplotypes, which could significantly increase spikelet number per spike in wheat. The KASP marker was also used to identify the superior haplotypes of Chinese wheat varieties/lines, Sichuan wheat varieties and world wheat varieties, and to explore their distribution and breeding utilization. The results reveal that the superior haplotypes are widely selected and bred in China and the world.

Key words: WAPO1, spikelet number per spike, superior haplotypes, geographical distribution, breeding utilization