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作物学报

作物学报 ›› 1986, Vol. 12 ›› Issue (02): 117-120.

• 研究论文 • 上一篇    下一篇

高赖氨酸玉米单粒鉴别技术初报——(Ⅰ)—Folin 酚比色法

王育东

  

  1. 中国农业科学院作物育栽培研究所

  • 收稿日期:1983-12-16 修回日期:1900-01-01 出版日期:1986-05-12 网络出版日期:1986-05-12

THE SINGLE KERNEL EVALUATION TECHNIQUES FOR THE HIGH-LYSINE CORN(Ⅰ)——FOLIN PHENOL COLORIMETRIC METHOD

Wang Yudong   

  1. Institute of Crop Breeding and Cultivation; Chinese Academy of Agricultural Sciences

  • Received:1983-12-16 Revised:1900-01-01 Published:1986-05-12 Published online:1986-05-12

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摘要：

在改造“奥帕克—2”玉米胚乳性状的育种工作中,需要对杂交后代进行单粒分析,以便挑选出那些既是硬胚乳又是赖氨酸含量高的籽粒。普通玉米的醇溶蛋白含量比高赖氨酸玉米高1倍左右。用灵敏度高的 Folin 酚比色法可以精确地反映出各籽粒间醇溶蛋白含量的差异,从而达到鉴别高赖氨酸玉米籽粒的目的。

Abstract:

In the breeding work to improve the characters of“Opaque-2”cornendosperm,it is necessary to analyze the hybrid progeny in order topick out the kernels of hard endosperm which contains high lysine.Thezein content of normal corns is about twice as much as high-lysine corn.The difference of zein content among the kernels can be distinguishedaccurately with the sensitive colorimetric method of Folin-phenol.Sothe high-lysine kernels can be picked out.

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