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作物学报 ›› 1987, Vol. 13 ›› Issue (01): 63-68.

• 研究论文 • 上一篇    下一篇

水稻种子蛋白质组分的双向电泳分析

孙崇荣; 李育庆; 汪景长; 朱俭   

  1. 复旦大学生物化学专业
  • 收稿日期:1986-06-05 修回日期:1987-01-25 出版日期:1987-02-12 网络出版日期:1987-02-12

TWO-DIMENSIONAL ELECTROPHORESIS OF SEED PROTEINS FROM RICE(ORYZA SATIVA L.)

Sun Chongrong; Li Yuqing; Wang Jinchang; Zhu Jian   

  1. Department of Biochemistry; Fudan University; Shanghai
  • Received:1986-06-05 Revised:1987-01-25 Published:1987-02-12 Published online:1987-02-12

摘要: 本文对双向电泳技术作了一些改进,应用于水稻种子胚乳及胚蛋白的组分分析,获得了较清晰而稳定的结果。水稻(Oryza sativa.L)品种珍汕97的胚乳蛋白可分析出约100种成分,而胚蛋白可分析出约550种成分,检测灵敏度为0.2μg 蛋白质/mm~2。通过对这些实验条件的比较,表明该技术对水稻等作物种子蛋白的分析是一种行之有效的方法。

Abstract: We have made some improvement to the technique of two-dimensionalpolyacrylamide gel electrophoresis.Clear and stable results were obtainedwhile we applied this technique to the analysis of rice(Oryza sativa L.)seed endosperm and seed embryo protein components.This technique hasresolved 100 different

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