应用丝瓜(Luffa Cylindrica Roem)伤流液提高水稻花药培养效果

作物学报 ›› 1991, Vol. 17 ›› Issue (05): 352-361.

应用丝瓜(Luffa Cylindrica Roem)伤流液提高水稻花药培养效果

张承妹; 章振华

上海市农业科学院作物育种栽培研究所

收稿日期:1991-01-02 修回日期:1991-08-01 出版日期:1991-09-12 网络出版日期:1991-09-12

Studies on the Effect of Bleeding Sap of Towel Gourd(Luffa Cylindrica Roem)on Rice Anther Culture

Zhang Chengmei; Zhang Zhenhua

Institute of crop breeding and Cultivation Shanghai Academy of Agriculture Sciences Shanghai; China

Received:1991-01-02 Revised:1991-08-01 Published:1991-09-12 Published online:1991-09-12

摘要/Abstract

摘要： 用15—20%(V/V)丝瓜伤流液加入培养基可以显著提高水稻花药培养力和花粉植株自然加倍的频率。诱导培养基中附加15—20%(V/V)丝瓜伤流液,形成愈伤组织百分率显著高于对照;粳稻的愈伤组织分化率高达95%,籼稻愈伤组织分化率可达70%,比对照提高1倍左右。花粉植株自然加倍的频率显著(p>0.01)高于对照。附加10%、15%、20%、25%(V/V)

关键词: 普通丝瓜, 伤流液, 水稻花粉植株, 花粉植株二倍化, 花药培养力

Abstract: The anther culture ability and natural diploidization of regenerated plants of rice wereSignificantly increased by the addition of 15-20%(V/V)bleeding sap of towel gourd intothe induction medium,Thus,the callus induction rate of the Supplemented media wasSignificantly higher than that of the check;t

Key words: Luffa Cylindrica Roem, pollen regenerated plant of rice, Diploidization of pollen regerated plant, Anther culture ability,

张承妹; 章振华. 应用丝瓜(Luffa Cylindrica Roem)伤流液提高水稻花药培养效果[J]. 作物学报, 1991, 17(05): 352-361.

Zhang Chengmei; Zhang Zhenhua. Studies on the Effect of Bleeding Sap of Towel Gourd(Luffa Cylindrica Roem)on Rice Anther Culture[J]. Acta Agron Sin, 1991, 17(05): 352-361.

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