不同激素条件下大豆原生质体培养和植株再生

作物学报 ›› 1993, Vol. 19 ›› Issue (04): 328-333.

不同激素条件下大豆原生质体培养和植株再生

吕慧能;盖钧镒;马育华;卫志明;许智宏

南京农业大学大豆研究所,南京,210014

收稿日期:1991-10-28 修回日期:1992-05-19 出版日期:1993-07-21 网络出版日期:1993-07-21
通讯作者: 吕慧能

Soybean Protoplast Culture under Different Hormone Conditions and Plantlet Regeneration

Lu Hui-neng Gai Jun-yi;Ma Yu-hua;Wei Zhi-ming;Xu Zhi-hong

Soybean Research Institute, Nanjing Agricultural University,210014

Received:1991-10-28 Revised:1992-05-19 Published:1993-07-21 Published online:1993-07-21
Contact: Lu Hui-neng

摘要/Abstract

摘要： 以栽培大豆(Glycine max(L.) Merr.)南农86-21、南农86-4、南农73-935为材料，用K8/K8p基本培养基比较了不同原生质体密度和不同激素种类、水平对未成熟子叶原生质体培养的影响，发现它们的差异表现在植板率、产生愈伤组织的速度和频率、愈伤组织的颜色和结构等几方面。对低激素来源南农73-935的愈伤组织的速度和频率、愈伤组

关键词: 大豆，原生质体培养，植株再生

Abstract: The influences of different protoplast density and different hormone types and levels on the culture of immature cotyledon protoplasts of soybean(Glycine max(L.)Merr.)on K8/K8p minimal medium were studied comparatively. The materials used include 3 soybean lines, Nannong 73-935, Nannong 86-21 and Na

Key words: Soybean, Protoplast culture, Plant regeneration

吕慧能;盖钧镒;马育华;卫志明;许智宏. 不同激素条件下大豆原生质体培养和植株再生[J]. 作物学报, 1993, 19(04): 328-333.

Lu Hui-neng Gai Jun-yi;Ma Yu-hua;Wei Zhi-ming;Xu Zhi-hong. Soybean Protoplast Culture under Different Hormone Conditions and Plantlet Regeneration[J]. Acta Agron Sin, 1993, 19(04): 328-333.

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