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作物学报

作物学报 ›› 1994, Vol. 20 ›› Issue (01): 1-7.

• 研究论文 •    下一篇

应用RFLP定位水稻的生育期基因

沈波;钱惠荣;王建林;郑康乐   

  1. 中国水稻研究所,浙江杭州,310006
  • 收稿日期:1992-07-01 修回日期:1993-02-18 出版日期:1994-01-12 网络出版日期:1994-01-12
  • 通讯作者: 沈波

Tagging Genes for Heading Date in Rice via Linkage to RFLP Markers

Shen Bo;Qian Hui-rong;Wang Jian-lin;Zheng Kang-le   

  1. China National Rice Research Institute,Hangzhou,310006
  • Received:1992-07-01 Revised:1993-02-18 Published:1994-01-12 Published online:1994-01-12
  • Contact: Shen Bo

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被引次数

63

摘要： 选用水稻RFLP图谱上的45个DNA随机克隆结合4种限制性内切酶,分析了广亲和品种Pecos,测验种秋光和南京11之间的多态性,发现其中15个探针能在这3个品种间检测到多态性。进一步用14个多态性探针和形态标记色素原基因C分析三交群体Pecos/南京11//秋光中标记与抽穗日数的共分离。结果表明：三交群体各单株抽穗期人布为双峰，早抽穗

关键词: 水稻，生育期，基因定位，RFLP

Abstract: A wide compatiblility variety Pecos, an indica variety Nanjing 11 and a japonica variety Akihikari were surveyed for restriction fragment length polymorphisms (RFLPs)using 45 mapped rice random genomio clones on ten chromosomes combined with 4 restriction enzymes(EcoR I,EcoR V,HindIII,Xba I).Fifteen

Key words: Rice, Heading date, Gene tagging, RFLP

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