大豆幼荚子叶原生质体培养及植株再生

作物学报 ›› 1994, Vol. 20 ›› Issue (06): 665-669.

大豆幼荚子叶原生质体培养及植株再生

肖文言;王连铮

中国农业科学院，北京，100081

收稿日期:1993-04-28 修回日期:1993-12-13 出版日期:1994-11-12 网络出版日期:1994-11-12
通讯作者: 肖文言

Protoplast Culture and Plant Regeneration of Immature Cotyledons of Soybean (Glycine max L.)

Xiao Wen-yan; Wang Lian-zheng

Chinese Academy of Agricultural Sciences, Bejing, 100081

Received:1993-04-28 Revised:1993-12-13 Published:1994-11-12 Published online:1994-11-12
Contact: Xiao Wen-yan

摘要/Abstract

摘要： 本文研究了13个栽培大豆（Glycine max L.）品种原生质体培养的再生能力。从大豆幼荚子叶酶解游离原生质体，用Gellan Gum进行株状包埋，悬浮在含2，4-D 0.1-0.2mg/L，BA0.5-1.0mg/L的改良MS液体培养基中，原生质体培养3天后开始第一次分裂，以后持续分裂。供试基因型间的10天植板率差异显著，变幅为33-67%。30天内形成大量的

关键词: 大豆，培养基，原生质体培养，植株再生

Abstract: Thirteen soybean (Glycien max L.) genotypes were evaluated for their regenerability from protoplasts. Protoplasts were isolated from immature cotyledons, buried in beads solidified with Gellan Gum, and cultured in modified MS liquid medium containing asparagines, glutamine, allantoin, and allantoic

Key words: Soybean(Glycine max L.), Medium, Protoplast culture, Plant regeneration

肖文言;王连铮. 大豆幼荚子叶原生质体培养及植株再生[J]. 作物学报, 1994, 20(06): 665-669.

Xiao Wen-yan; Wang Lian-zheng. Protoplast Culture and Plant Regeneration of Immature Cotyledons of Soybean (Glycine max L.)[J]. Acta Agron Sin, 1994, 20(06): 665-669.

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