实时荧光定量PCR技术在转基因玉米检测中的应用研究

作物学报 ›› 2004, Vol. 30 ›› Issue (06): 602-607.

实时荧光定量PCR技术在转基因玉米检测中的应用研究

陈颖;徐宝梁;苏宁;葛毅强;王曙光

中国进出口商品检验技术研究所，北京100025

收稿日期:2002-12-09 修回日期:2003-08-12 出版日期:2004-06-12 网络出版日期:2004-06-12
通讯作者: 陈颖

Real-time Quantitative PCR Detection of Genetically Modified Maximizerâ Maize and YieldGardâ Maize

CHEN Ying;SU Ning;XU Bo-Liang;GE Yi-Qiang;WANG Shu-Guang

China Import and Export Commodity Inspection Technology Institute, Beijing 100025

Received:2002-12-09 Revised:2003-08-12 Published:2004-06-12 Published online:2004-06-12
Contact: CHEN Ying

摘要/Abstract

摘要： 采用实时荧光定量PCR技术，通过使用特异的引物和探针，对玉米中的内源基因Invertase和转基因玉米Mon 810、Event 176中的外源基因进行了定量检测，建立了商业化转基因玉米Mon 810 (YieldGard)和Event 176 (Maximizer)的定量PCR检测方法。该方法的检测灵敏度小于0.01％，是国际上设定的转基因最低限量的100倍。

关键词: 定量PCR技术, 转基因玉米Mon 810 (YieldGard), 转基因玉米Event 176 (Maximizer), 转基因检测

Abstract: A reliable and simple Real-time quantitative polymerase chain reaction method for detection of genetically modified maize with ABI Prism 7 700 was established in this paper. A set of PCR primers and probes was designed specific to transgenic commercial maize Mon 810 and Event 176. The detection limi

Key words: Real-time quantitative PCR, YieldGard ®, Maize Mon 810, Maximizer ®, Maize Event 176, GMO detection

中图分类号:

S513

陈颖;徐宝梁;苏宁;葛毅强;王曙光. 实时荧光定量PCR技术在转基因玉米检测中的应用研究[J]. 作物学报, 2004, 30(06): 602-607.

CHEN Ying;SU Ning;XU Bo-Liang;GE Yi-Qiang;WANG Shu-Guang. Real-time Quantitative PCR Detection of Genetically Modified Maximizerâ Maize and YieldGardâ Maize[J]. Acta Agron Sin, 2004, 30(06): 602-607.

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