“十五”大豆创新种质和1968—1995年间育成品种的SSR遗传结构及遗传多样性分析

作物学报 ›› 2007, Vol. 33 ›› Issue (10): 1630-1636.

“十五”大豆创新种质和1968—1995年间育成品种的SSR遗传结构及遗传多样性分析

李英慧^1,**, 刘燕 ^1,**, 关荣霞¹ , 魏淑红², 杨光宇³, 周新安⁴, 张孟臣⁵ , 杨春燕⁵, 朱保葛⁶, 李卫东⁷, 刘学义⁸, 徐冉⁹, 孙君明¹, 朱申龙¹⁰,

1中国农业科学院作物科学研究所/国家农作物基因资源与遗传改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室，北京100081;2黑龙江省农科院育种研究所, 黑龙江哈尔滨150086；3吉林省农科院大豆所，吉林公主岭136100；4中国农科院油料作物研究所，湖北武汉430062；5河北省农科院粮作所，河北石家庄050031；6中国科学院遗传发育所，北京100101；7河南省农科院棉油所，河南郑州450002；8山西省农科院经作所，山西汾阳032200；9山东省农业科学院作物研究所，山东济南250100；10浙江省农科院作物所，浙江杭州310021；11南京农业大学农学院，江苏南京210095

收稿日期:2006-12-02 修回日期:1900-01-01 出版日期:2007-10-12 网络出版日期:2007-10-12
通讯作者: 邱丽娟

Genetic Structure and Diversity of Both Enhanced Germplasms Developed during 10th Five Year Plan and Modern Cultivars Released during 1968–1995 in China

LI Ying-Hui¹, LIU Yan¹, GUAN Rong-Xia¹, LIU Zhang-Xiong¹, WEI Shu-Hong², YANG Guang-Yu³, ZHOU Xin-An⁴, ZHANG Meng-Chen⁵, YANG Chun-Yan⁵, ZHU Bao-Ge⁶, LI Wei-Dong⁷, LIU Xue-Yi <SUP

1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, / Key Laboratory of Germplasm & Biotechnology, Ministry of Agriculture / The National Key Facility for Crop Gene Resources and Genetic Improvement 100081 Beijing; 2 Helongjiang Academy of Agricultural Sciences, Haerbin 150086, Helongjiang; 3 Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin; 4 Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, Hubei, P. R. China; 5 Hebei Academy of Agricultural Sciences, Shijiazhuang 050031, Hebei; 6 Chinese Academy of Sciences, 100101 Beijing; 7 Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan; 8 Shanxi Academy of Agricultural Sciences, Fenyang 032200, Shanxi; 9 Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong; 10 Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,; 11 Nanjing Agricultural University, Nanjing 210095, Jiangsu, China

Received:2006-12-02 Revised:1900-01-01 Published:2007-10-12 Published online:2007-10-12
Contact: QIU Li-Juan

摘要/Abstract

摘要：

对22份“十五”攻关培育的创新种质和22份大豆育成品种进行了24个SSR标记的分析比较，目的是在分子水平上阐明创新种质的遗传结构特点，为拓宽我国大豆育成品种遗传基础及亲本选择提供理论依据。本研究在24个SSR位点共发现231个等位变异，其中15.8%（36个等位变异）为创新种质所特有，特别是在与大豆胞囊线虫紧密连锁的Satt309位点上验证了一个我国独有的等位变异。结合UPGMA和Model-based聚类结果，将创新种质和育成品种分为4组，第Ⅰ组由13份来自东北和山西的创新种质组成；第Ⅱ组由8份来自东北的育成品种组成；第Ⅲ组由8份来自黄淮海和南方的大豆种质组成，其中创新种质和育成品种各为4份；第Ⅳ组由4份育成品种组成，分别来自吉林、黑龙江、河南和山西。遗传多样性分析结果表明，利用国外种质和野生大豆创造的创新种质丰富了东北地区育成品种的遗传多样性。因此，应加强利用国外种质、我国栽培大豆地方品种和野生大豆等优异资源，在创造优异大豆新种质的同时，拓宽我国大豆的遗传基础。

Abstract:

To clarify the patterns of genetic structure, 22 relased modern cultivars and 22 new enhanced germplasms were analyzed with 24 SSR markers. Among the total of 231 alleles, 36 unique alleles were found only in new enhanced germplasms. At Satt309 locus, which closely linked with soybean cyst nematode resistance, an allele, which only was found in Chinese soybean landrace, was confirmed. Combining the results obtained by using UPGMA method and model-based method, four distinct clusters were discovered. Cluster Ⅰ was comprised of 13 new enhanced germplasms from Northeast region and Shanxi province. Cluster Ⅱ included 8 released cultivars from Northerneast region. Cluster Ⅲ consisted of 4 new enhanced germplasms and 4 released cultivars from Huanghuaihai and Southern region. Cluster Ⅳ contained 4 relased cultivars from Jilin, Heilongjiang, Shanxi and Henan provinces. The results of genetic diversity analysis showed that the new enhanced germplasms, which utilized foreign germplasms and wild species as parent, broadened the genetic base of released cultivars in northeast region.

Key words: New enhanced germplasms, Released cultivar, SSR marker, Genetic diversity

李英慧;刘燕;关荣霞;魏淑红;杨光宇;周新安;张孟臣;杨春燕;朱保葛;李卫东;刘学义;徐冉;孙君明;朱申龙. “十五”大豆创新种质和1968—1995年间育成品种的SSR遗传结构及遗传多样性分析[J]. 作物学报, 2007, 33(10): 1630-1636.

LI Ying-Hui; LIU Yan; GUAN Rong-Xia; LIU Zhang-Xiong; WEI Shu-Hong; YANG Guang-Yu; ZHOU Xin-An; ZHANG Meng-Chen; YANG Chun-Yan; ZHU Bao-Ge6; LI Wei-Dong7; LIU Xue-Yi <SUP. Genetic Structure and Diversity of Both Enhanced Germplasms Developed during 10th Five Year Plan and Modern Cultivars Released during 1968–1995 in China[J]. Acta Agron Sin, 2007, 33(10): 1630-1636.

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