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作物学报 ›› 2010, Vol. 36 ›› Issue (4): 680-687.doi: 10.3724/SP.J.1006.2010.00680

• 研究简报 • 上一篇    下一篇

花后不同时期油菜籽饼粕赖氨酸含量的遗传分析

陈国林1,2,吴建国1,张莉1,石春海1,*   

  1. 1浙江大学农业与生物技术学院，浙江杭州310029;2浙江林学院农业与食品学院,浙江临安300300
  • 收稿日期:2009-09-10 修回日期:2009-12-25 出版日期:2010-04-12 网络出版日期:2010-03-03
  • 通讯作者: 石春海,E-mail:chhshi@zju.edu.cn;Tel:0571-86971691
  • 基金资助:

    本研究由浙江省科技厅面上科研农业项目(2008C22084), 教育部高等学校骨干教师资助计划项目和浙江省“151人才工程”专项基金项目资助。

Genetic Analysis for Lysine Content of Rapeseed Cake (Brassic napus L.) at Different Periods after Anthesis

CHEN Guo-Lin1,2,WU Jian-Guo1,ZHANG Li1,SHI Chun-Hai1,*   

  1. 1College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China;2School of Biosystems Engineering and Food Sciences,Zhejiang Forestry University,Lin'an 330330,China
  • Received:2009-09-10 Revised:2009-12-25 Published:2010-04-12 Published online:2010-03-03
  • Contact: SHI Chun-Hai,E-mail:chhshi@zju.edu.cn;Tel:0571-86971691

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被引次数

2

摘要：

采用条件和非条件数量遗传分析方法，利用不同环境条件下的两年试验数据，分析了胚(子叶)、细胞质和母体植株等不同遗传体系的遗传主效应及其环境互作效应对油菜籽饼粕赖氨酸含量的影响。结果表明，油菜籽饼粕赖氨酸含量在各个发育时期的表现同时受制于不同遗传体系的遗传主效应和环境互作效应，且以环境互作效应为主，故油菜籽发育过程中不同遗传体系基因的表达会受到环境条件的显著影响。在不同遗传体系的基因效应中，除花后36 d的胚主效应较大外，各发育时期以母体效应为主，其中母体显性主效应和母体加性互作效应的作用尤为明显。条件遗传分析表明，花后23~29 d控制赖氨酸含量的数量基因表达最为活跃；一些基因效应在不同发育时期还存在着间断性表达的现象。油菜籽饼粕赖氨酸含量的狭义遗传率较高，多数发育时期是以母体遗传率为主，根据母体植株上油菜籽饼粕赖氨酸含量的总体表现进行选择可以取得较好的改良效果。

关键词: 油菜籽, 赖氨酸含量, 发育遗传, 遗传主效应, 环境互作效应, 遗传率

Abstract:

Both conditional and unconditional analysis methods were applied to estimate the genetic main effects and the genotype × environment (GE) interaction effects from embryo (cotyledon), cytoplasmic and maternal plant at different periods after anthesis for lysine content of rapeseed cake by using two-year experimental data. Lysine content (LC) in rapeseed cake was simultaneously controlled by the genetic main effects and their GE interaction effects from different genetic systems, especially for the GE interaction effects. Therefore, the expression of genes conferring LC in the different genetic systems could be significantly influenced by the environments. Among the different genetic components, the maternal effects on LC of rapeseed cake were the most important at all periods after anthesis except for the larger embryo main effects at 36 day after flowering, in which both maternal dominance effect and maternal additive interaction effect were more visible. It was found that the new expression of quantitative genes on LC trait was most active at 23–29 days after flowering by using the conditional statistical analysis method. The discontinuity of gene expression in the developmental process of rapeseed was also observed. The total narrow-sense heritability of LC was high, of which the maternal heritability was important at most periods after anthesis. It was suggested that LC could be efficiently improved when selection is based on the holistic performance of maternal plants in generations. The study further suggested that Eyouchangjia and Gaoyou 605 were better than other parents for increasing LC of progenies.

Key words: Rapeseed(Brassica napus L.), Lysine content, Developmental genetics, Genetic main effects, Genotype×environment interaction effects, Heritability

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