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作物学报 ›› 2011, Vol. 37 ›› Issue (07): 1125-1133.doi: 10.3724/SP.J.1006.2011.01125

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

CIMMYT小麦材料的苗期和成株抗叶锈病鉴定

韩烨1,何中虎2,3,夏先春2,李星1,李在峰1,*,刘大群1,*   

  1. 1 河北农业大学植物保护学院 / 河北省农作物病虫害生物防治工程技术研究中心，河北保定 071001；2 中国农业科学院作物科学研究所 / 国家小麦改良中心，北京 100081；3 CIMMYT中国办事处，北京 100081
  • 收稿日期:2010-12-28 修回日期:2011-03-27 出版日期:2011-07-12 网络出版日期:2011-05-11
  • 通讯作者: 李在峰, E-mail: lzf7551@yahoo.com.cn, Tel: 0312-7528500; 刘大群, E-mail: ldq@hebau.edu.cn
  • 基金资助:

    本研究由引进国外先进农业科学技术计划(948计划)项目(2006G2), 国家自然科学基金项目(30971772, 30700505), 河北省自然科学基金项目(C2010000702)资助。

Seedling and Slow Rusting Resistances to Leaf Rust in CIMMYT Wheat Lines

HAN Ye1,HE Zhong-Hu2,3,XIA Xian-Chun2,LI Xing1,LI Zai-Feng1,*,LIU Da-Qun1,*   

  1. 1 College of Plant Protection, Agricultural University of Hebei / Biological Control Center of Plant Disease and Plant Pests of Hebei Province, Baoding 071000, China; 2 Institute of Crop Sciences / National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 CIMMYT China Office, Beijing 100081, China
  • Received:2010-12-28 Revised:2011-03-27 Published:2011-07-12 Published online:2011-05-11
  • Contact: 李在峰, E-mail: lzf7551@yahoo.com.cn, Tel: 0312-7528500; 刘大群, E-mail: ldq@hebau.edu.cn

PDF

1443

被引次数

29

摘要： 选择来自CIMMYT的103个小麦品种(系)及35个含有已知抗叶锈基因的对照品种，苗期接种17个中国小麦叶锈菌小种以鉴定这些品种中可能含有的抗叶锈病基因, 并于2008-2009和2009-2010连续2年对这些材料进行成株抗叶锈病鉴定。通过苗期鉴定结合系谱分析和分子检测，在46个品种中共鉴定出Lr26、Lr34、Lr42和Lr47等4个抗叶锈病基因，其中9个品种携带Lr26基因，28个品种含有成株抗叶锈病基因Lr34基因，Lr42可能存在于11个品种中，还有2个材料可能含有Lr47，其他57个品种(系)对供试的15个小种多数表现为高抗，没有鉴定出已知的抗叶锈病基因。通过两年的田间抗叶锈病鉴定共筛选出46个表现慢锈的品种。苗期和田间结果表明，CIMMYT材料中含有丰富的对我国叶锈菌小种有效的苗期和成株抗叶锈基因，这些材料均可应用于我国小麦的抗叶锈病育种。

关键词: CIMMYT小麦, 叶锈病, 基因鉴定, 慢锈性

Abstract: CIMMYT wheat has played an important role in wheat breeding in China. Identification of leaf rust resistance genes in CIMMYT wheat is important for developing wheat cultivars with resistance to leaf rust in China. A total of 103 CIMMYT wheat lines and a set of 35 differential lines, mostly near-isogenic lines in the background of Thatcher with known leaf rust resistance genes were inoculated with 15 Chinese pathotypes of Puccinia triticina for postulating leaf rust resistance genes in CIMMYT wheats at the seedling stage. These genotypes were also planted in the field for characterization of slow rusting responses to leaf rust in the 2008–2009 and 2009–2010 cropping seasons.Four leaf rust resistance genes Lr26, Lr34, Lr42, and Lr47 were postulated in 46 genotypes through seedling test, pedigree analysis and molecular maker detection. Resistance gene Lr26 was present in nine accessions. Lr34 was identified in 28 lines. Lr42 was found in eleven lines and two lines might contain Lr47. Known resistance genes were not identified in the other 57 accessions and these lines were resistant to most of pathotypes tested. Forty-six genotypes showed slow leaf rusting resistance in the two cropping seasons. The results from seedling and field tests showed that CIMMYT wheats carry abundant effective seedling and adult plant resistance genes, and can be used in breeding resistant cultivars for durable control of wheat leaf rust in China.

Key words: CIMMYT wheat, Leaf rust, Identification of genes, Slow-rusting resistance

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