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作物学报 ›› 2017, Vol. 43 ›› Issue (02): 171-178.doi: 10.3724/SP.J.1006.2017.00171

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

雄性不育小麦BS210育性转换特性

孙辉**,张风廷**,王永波,叶志杰,秦志列,白秀成,杨吉芳,高建刚,赵昌平*   

  1. 赵昌平, E-mail: cp_zhao@vip.sohu.com
  • 收稿日期:2016-03-15 修回日期:2016-11-03 出版日期:2017-02-12 网络出版日期:2016-11-18
  • 基金资助:

    本研究由国家科技支撑计划项目(2014BAD01B05), 北京市农林科学院青年科研基金(QNJJ201307), 北京市农林科学院科技创新基金(CXJJ201307), 北京市科技计划(Z141100002314017)和北京市农林科学院科技创新能力建设专项(KJCX20150705)资助。

Fertility Alteration in Male Sterile Line BS210 of Wheat

SUN Hui**,ZHANG Feng-Ting**,WANG Yong-Bo,YE Zhi-Jie,QIN Zhi-Lie,BAI Xiu-Cheng,YANG Ji-Fang,GAO Jian-Gang,ZHAO Chang-Ping*   

  1. 赵昌平, E-mail: cp_zhao@vip.sohu.com
  • Received:2016-03-15 Revised:2016-11-03 Published:2017-02-12 Published online:2016-11-18
  • Supported by:

    This study was supported by the National Key Technology R&D Development Program of China (2014BAD01B05), the Youth Research Fund of the Beijing Academy of Agriculture and Forestry Sciences (QNJJ201307), the Technology Innovation Program of the Beijing Academy of Agriculture and Forestry Sciences (CXJJ201307), the Beijing Science and Technology (Z141100002314017), and the Special Project for Enhancing Innovation Ability of the Beijing Academy of Agriculture and Forestry Sciences (KJCX20150705).

摘要:

二系法杂交小麦属我国原创性成果, 温光敏雄性不育小麦是二系法杂交小麦应用的核心。BS210是本课题组选育的小麦雄性不育材料, 2012—2014年通过3个生态区分期播种试验和人工气候箱温光调控试验, 研究了BS210的育性转换特性。在不育制种生态区, 适期播种条件下, BS210的不育度可达95%~100%, 其安全制种播种时期为安徽阜阳9月30日至10月10日、云南丽江9月30日;在可育繁种生态区, 晚播条件下, BS210的自交结实率可达70%以上, 其安全繁种播种时期为北京海淀11月10日、北京顺义9月30日至11月10日。BS210以温度敏感为主, 光照时长对BS210育性影响较小, 温度转换敏感期为药隔至单核期。在光照10.0~12.5 h时, 其温度转换阈值为10~12°C;在光照14 h时, 其温度转换阈值为8~10°C。在短日低温条件下, 花粉粒碘染以染败为主, 表现为不育;在长日高温条件下, 花粉粒碘染为圆形、黑染, 表现为可育。通过明确BS210育性转换特性, 为指导BS210安全制繁种生态区的选择提供理论依据。

关键词: 二系杂交小麦, 温光敏雄性不育, 育性转换

Abstract:

Two-line hybrid wheat is an innovation in China and its critical technique is the application of thermo-photo-sensitive male sterile line. BS210 is a male sterile wheat line developed by our group. We studied the fertility transition characteristics of BS210 with differentially sowing tests at three ecological locations and contorlled experiments in growth chambers from 2012 to 2014. In the hybrid seed-produing locations, BS210 showed sterility of 95–100% under suitable sowing conditions with the safe sowing dates from September 30th to October 10th in Fuyang of Anhui and September 30th in Lijiang of Yunnan. In the self-reproducting locations, BS210 showed fertility of 70% under late sowing conditions with the safe sowing dates of November 10th in Haidian of Beijing and from September 30th to November 10th in Shunyi of Beijing. The fertility alteration of BS210 was induced mainly by temperature rather than photoperiod. The phase from pollen mother cell formation to mono-nucleate was sensitive to fertility alteration of BS210. The critical temperature of alteration was 10–12°C under the photoperiod of 10.0–12.5 h and 8–10°C under the photoperiod of 14 h. The pollen-staining observation indicated that the sterile pollen grains belonged to stained abortion type under short-day and low-temperature condition and the fertile pollen grains were round in shape and dark blue in color under long-day and high-temperature condition. Defining the fertility transition characteristics of BS210 will provide theoretical guidelines for selecting suitable ecological zone to ensure safe seed production.

Key words: Two-line hybrid wheat, Thermo-photo-sensitive male sterile line, Fertility alteration

[1]赵昌平. 中国杂交小麦研究现状与趋势. 中国农业科技导报, 2010, 12(2): 5–8
    Zhao C P. Status and trends of hybrid wheat research in China. J Agric Sci Technol, 2010, 12(2): 5–8 (in Chinese with English abstract)
[2]Kinara H. Substitution of nucleus and its effects on genome manifestations. Cytologia, 1951, 16: 177–193
[3]Wilson J A, Ross W M. Male sterility interaction of the Triticum aestivum nucleus and Triticum timopheevii cytoplasm. Wheat Info Serv, 1962, 14: 29–30
[4]Sasakuma T I, Ohtsuka. Cytoplasmic effects of Aegilops species having D genome in wheat: I. Cytoplasmic differentiation among five species regarding pistilbody-induction. Seiken Ziho, 1979, 27/28: 59–65
[5]Mural K, Tsunewaki K. Photoperiod-sensitive cytoplasmic male sterility in wheat with Aegilops crossa cytoplasm. Euphytlca, 1993, 67: 41-48
[6]Murai K. Factors responsible for levels of male sterility in photoperiod-sensitive cytoplasmic male sterile (PCMS) wheat lines. Euphytica, 2001, 117: 111–116
[7]Murai K, Tsutui I, Kawanishi Y, Ikeguchi S, Yanaka M, Ishikawa N. Development of photoperiod-sensitive cytoplasmic male sterile (PCMS) wheat lines showing high male sterility under long-day conditions and high seed fertility under short-day conditions. Euphytica, 2008, 159: 315–323
[8]何觉民, 戴君惕, 邹应斌, 周美兰, 张海清, 刘雄伦. 两系杂交小麦研究: I. 生态雄性不育小麦的发现、培育及其利用价值. 湖南农业科学, 1992, (5): 1–3
He J M, Dai J T, Zou Y B, Zhou M L, Zhang H Q, Liu X L. Research in two-line male sterile wheat, discovering, cultivating and value of ecological male sterile wheat. Hunan Agricultural Sciences, 1992, (5): 1–3 (in Chinese)
[9]谭昌华, 余国东, 扬沛丰, 张宗华, 潘鹰, 郑坚. 重庆温光型核不育小麦的不育性研究初报.西南农业学报, 1992, (4): 1–6
Tang C H, Yu G D, Yang P F, Zhang Z H, Pan Y, Zheng J. Research of thermo-photo- sensitive male sterile line of wheat in Chongqing. Southwest China J Agric Sci, 1992, (4): 1–6 (in Chinese with English abstact)
[10]赵昌平, 王新, 张风廷, 叶志杰, 戴惠君. 杂种小麦的利用现状与光温敏二系法. 北京农业科学, 1999, 17(2): 3–5
    Zhao C P, Wang X, Zhang F T, Ye Z J, Dai H J. Advances of hybrid wheat and two-line system of photo-thermo sensitivity. Beijing Agric Sci, 1999, 17(2): 3–5 (in Chinese with English abstract)
[11]荣德福, 李少华, 郭拥军, 周世文. 两极光温敏感型小麦雄性不育系337S的选育. 湖北农业科学, 2001, (5): 13–16
Rong D F, Li S H, Guo Y J, Zhou S W. Breeding of double pole photo-thermo-sensitive type male sterile line 337s of wheat. Hubei Agric Sci, 2001, (5): 13–16 (in Chinese)
[12]何觉民, 戴君惕, 邹应斌, 张海清, 刘雄伦, 欧仕益, 周美兰, 杨友才, 曾利荣. 生态雄遗传性不育理论与两系杂交作物II: 选育生态遗传雄性不育系的一般方法. 湖南农学院学报, 1994, 20: 412–418
He J M, Dai J T, Zou Y B, Zhang H Q, Liu X L, Ou S Y, Zhou M L, Yang Y C, Zeng L R. The theory of ecological genetic sterility and hybrid crop produced by two-line method. J Hunan Agric Coll, 1994, 20: 412–418 (in Chinese with English abstract)
[13]张建奎, 何立人, 冯丽, 管健. 日长和温度对雄性核不育小麦C49S育性转换的影响. 西南农业大学学报, 1999, 21: 518–521
Zhang J K, He L R, Feng L, Guan J. Effects of photoperiod on fertility transformation in thermo-photo-sensitive male sterile wheat. J Southwest China Agric Univ, 1999, 21: 518–521 (in Chinese with English abstract)
[14]周美兰, 何觉民, 邹应斌, 刘雄伦, 李海林. 光温敏核不育小麦育性转换条件的研究. 湖南农业大学学报, 1996, 22: 231–235
Zhou M L, He J M, Zou Y B, Liu X L, Li H L. A study on fertility transformation conditions of photoperiod-temperature sensitive genic male-sterile wheat. J Hunan Agric Univ, 1996, 22: 231–235 (in Chinese with English abstract)
[15]周美兰, 唐启源, 程尧楚, 何觉民. 光温敏核不育小麦ES-10雄性败育机制研究. 湖南农业大学学报, 1997, 23: 117–121
Zhou M L, Tang Q Y, Cheng Y C, He J M. The mechanism of male abortion in the photo-thermo-sensitive genic male sterile line ES-10 of wheat. J Hunan Agric Univ, 1997, 23: 117–121 (in Chinese with English abstract)
[16]何蓓如, 董普辉, 宋喜悦, 马翎健, 胡银岗, 蒋通关, 王俊鹏, 李宏斌. 小麦温度敏感不育系A3314温敏特性研究. 麦类作物学报, 2003, 23(1): 1–6
He B R, Dong P H, Song X Y, Ma L J, Hu Y G, Jiang T G, Wang J P, Li H B. Study on the thermo-sensitivity of thermo-sensitive male-sterile wheat line A3314. J Triticeae Crops, 2003, 23(1): 1–6 (in Chinese with English abstract)
[17]Song X Y, Zhang L L, Zeng J L, Qian H H, Li H B, He B R. Development of thermo-sensitive cytoplasmic male sterile (TCMS) lines of wheat characterized by complete male sterility at lower-temperatures and partially restored fertility at higher-temperatures. Euphytica, 2013, 192: 393–399
[18]张自阳, 胡铁柱, 冯素伟, 李笑慧, 李淦, 茹振钢. 温敏核雄性不育小麦BNS的育性转换规律初探. 河南农业科学, 2010, (7): 5–9
Zhang Z Y, Hu T Z, Feng S W, Li X H, Li G, Ru Z G. A preliminary study on fertility alteration of themo-sensitive genic male sterile wheat line BNS. Henan Agric Sci, 2010, (7): 5–9 (in Chinese with English abstract)
[19]王茂婷, 高庆荣, 孙正娟, 袁凯, 于松, 张宝雷, 李楠楠, 茹振钢. BNS小麦穗分化进程与其雄性不育性的表现. 分子植物育种, 2011, 9: 294–301
Wang M T, Gao Q R, Sun Z J, Yuan K, Yu S, Zhang B L, Li N N, Ru Z G. Spike differentiation process and the male sterility of BNS in wheat. Mol Plant Breed, 2011, 9: 294–301 (in Chinese with English abstract)
[20]Li Y F, Zhao C P, Zhang F T, Sun H. Fertility alteration in the photo-thermo-sensitive male sterile line BS20 of wheat (Triticum aestivum L.). Euphytica, 2006, 151: 207–213
[21]Guo R X, Sun D F. Inheritance of thermo-photoperiod sensitive male sterility in wheat. Austr J Agric Res, 2006, 57: 187–192
[22]张建奎, 董静, 余国东, 宗学凤, 李伯群. 光照强度对温光敏细胞核雄性不育小麦育性的影响. 植物遗传资源学报, 2011, 12: 301–306
Zhang J K, Dong J, Yu G D, Zong X F, Li B Q. Effect of light intensity on fertility alternation of themo-photo-sensitive genic male sterile wheat. J Plant Genet Resour, 2011, 12: 301–306 (in Chinese with English abstract)
[23]周美兰, 何觉民, 唐启源. 光周期对光温敏核不育小麦ES-8育性转换特性的影响. 麦类作物, 1997, 17(2): 9–12
Zhou M L, He J M, Tang Q Y. The effect on the transformation of the male fertility of ES-8 to photoperiod. Tritical Crops, 1997, 17(2): 9–12 (in Chinese)
[24]杨木军, 顾坚, 刘琨, 李绍祥, 田玉仙, 杨和仙, 周金生, 刘大钧, 陈佩度. 小麦温光敏雄性核不育系K78S在云南的生态适应性研究. 作物学报, 2006, 32: 1618–1624
Yang M J, Gu J, Liu K, Li S X, Tian Y X, Yang H X, Zhou J S, Liu D J, Chen P D. Ecological adaptability of thermo-photo-sensitive genic male sterile wheat K78S in Yunnan province. Acta Agron Sin, 2006, 32: 1618–1624 (in Chinese with English abstract)
[25] 宋国琦, 胡银岗, 林凡云, 董普辉, 马翎健, 宋喜悦, 何蓓如. YS型小麦温敏雄性不育系A3017控温条件下的花粉育性比较. 麦类作物学报, 2006, 26(1): 17–20
Song G Q, Hu Y G, Lin F Y, Dong P H, Ma L J, Song X Y, He B R. Comparison on the pollen fertility of YS type thermo-sensitive male-sterile wheat line A3017 under artificial temperature conditions. J Triticeae Crops, 2006, 26(1): 17–20 (in Chinese with English abstract)
[26]贺晓敏, 周美兰, 余传启, 茹振钢, 杨庚武, 孔得群, 李雪梅, 陈其敏. 温敏雄性不育系小麦BNS败育的细胞学观察. 安徽农业科学, 2013, 41: 9882–9885
He X M, Zhou M L, Yu C Q, Ru Z G, Yang G W, Kong D Q, Li X M, Chen Q M. Cytological observation of male abortion of thermo-sensitive male sterile wheat BNS. J Anhui Agric Sci, 2013, 41: 9882–9885 (in Chinese with English abstract)

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[2] 张建奎;董静;宗学凤;余国东;戴秀梅;阮仁武. 温光敏核不育小麦C412S的育性转换及其APRT基因的表达[J]. 作物学报, 2009, 35(4): 662-671.
[3] 周琳璘;宋国琦;李红燕;胡银岗;何蓓如. 一个与小麦雄性不育育性转换相关的MADS-box转录因子基因[J]. 作物学报, 2008, 34(04): 598-604.
[4] 何强;陈立云;邓华凤;唐文邦;肖应辉;袁隆平. 水稻C815S及其同源株系的育性光温特性[J]. 作物学报, 2007, 33(02): 262-268.
[5] 汤继华;赫忠友;陈伟程;谭树义;谢惠玲;李永亮. 玉米温敏核雄性不育育性转换与内源激素的关系[J]. 作物学报, 2003, 29(03): 336-338.
[6] 邹江石;姚克敏;邓芳萍. 培矮64S的育性特征及其安全使用技术[J]. 作物学报, 2003, 29(01): 87-92.
[7] 陆燕鹏;万邦惠;陈雄辉;彭海峰;梁克勤. 广州的短日照对短光低温不育水稻宜D1S育性转换的影响[J]. 作物学报, 2001, 27(02): 216-221.
[8] 孙宗修;程式华;闵绍楷;熊振民;应存山;斯华敏;杨仁崔;梁康迳;王乃元. 光敏核不育水稻的光温反应研究[J]. 作物学报, 1993, 19(01): 83-87.
[9] 薛光行;陈平. 温度对光敏感雄性核不育水稻育性转换的影响研究[J]. 作物学报, 1992, 18(06): 429-438.
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