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作物学报

作物学报 ›› 2017, Vol. 43 ›› Issue (08): 1115-1121.doi: 10.3724/SP.J.1006.2017.01115

• 综述 • 上一篇    下一篇

寄主诱导的基因沉默提高植物真菌病害抗性研究进展

程伟1,李和平2,何水林1,廖玉才2,*   

  1. 1福建农林大学作物科学学院/教育部作物遗传改良和综合利用重点实验室,福建福州350002;2华中农业大学植物科学技术学院/麦类作物分子生物技术实验室,湖北武汉430070
  • 收稿日期:2017-02-21 修回日期:2017-05-14 出版日期:2017-08-12 网络出版日期:2017-05-16
  • 通讯作者: 廖玉才,E-mail:yucailiao@mail.hzau.edu.cn,Tel:027-87283008
  • 基金资助:

    本研究由国家转基因生物新品种培育重大专项(2016ZX08002001-003),国家自然科学基金项目(31601761)和福建省中青年教师教育科研项目(JAT160180)资助。

Progress in Enhancement of Plant Resistance against Fungal Diseases through Host-Induced Gene Silencing

CHENGWei1,LIHe-Ping2,HEShui-Lin1,LIAOYu-Cai2,*   

  1. 1 National Education Minister Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization / College of CropScience, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Molecular Biotechnology Laboratory of Triticeae Crops / College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2017-02-21 Revised:2017-05-14 Published:2017-08-12 Published online:2017-05-16
  • Contact: LiaoYucai ,E-mail:yucailiao@mail.hzau.edu.cn,Tel:027-87283008
  • Supported by:

    ThisresearchwassupportedbytheNationalMajorProjectforDevelopingNewGMCrops(2016ZX08002001-003),theNationalNaturalScienceFoundationofChina(31601761),andtheResearchProjectforYoungTeachersofFujianProvince(JAT160180).

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摘要：

寄主诱导的基因沉默(host-induced gene silencing, HIGS)以病原菌生长发育、产孢繁殖、侵染或致病过程中的关键基因作为靶点，在寄主植物中表达针对靶基因的RNAi构建体，在病原菌侵染植物的过程中，摄入相应的dsRNA或siRNA分子，通过识别、结合特异核苷酸序列，干扰病菌靶基因表达，从而抑制病菌侵染和扩展，使植物表现抗病。这项技术为培育基于病原菌特异序列的植物抗病性奠定了基础，显示了巨大的应用潜力。本文综述了利用HIGS技术提高植物真菌病抗性的最新研究进展，总结了国内外利用这项技术改良植物真菌病害抗性的主要策略、技术路线，展望了发展应用前景。

关键词: RNA干扰, 寄主诱导的基因沉默(HIGS), 真菌病害, 转基因植物

Abstract:

Host-induced gene silencing (HIGS) uses the key genes that are involved in growth and development, sporulation and propagation, and pathogenesis of pathogens as targets, and expresses the RNAi constructs in host plants targeting to the target genes; during infection of plants, pathogens take up dsRNA or siRNA molecules, specifically bind to nucleotide sequences after recognition, and interfere the expression of the target genes, thereby inhibiting infection and spreading of invaded pathogens and thus conferring resistance phenotypes in plants. This technology serves as the solid foundation of plant resistance based on pathogen-specific sequences and has great potential for application in crop improvement against fungal diseases. In this review, recent advances in methodologies, technological routes and application of the HIGS strategy in plants against invading fungal pathogens are summarized; prospects for the future in HIGS-based plant resistance to fungal pathogens were also discussed.

Key words: RNA interfering, Host-induced gene silencing (HIGS), Fungal disease, Transgenic plants

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