多环境下稻米粒重的QTL定位

作物学报 ›› 2007, Vol. 33 ›› Issue (11): 1771-1776.

多环境下稻米粒重的QTL定位

吴秀菊¹;万向元¹;江玲¹;肖应辉¹;刘世家¹;陈亮明¹;翟虎渠³;万建民^1,2,*

1南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心，江苏南京 210095；2中国农业科学院作物科学研究所，北京 100081；3中国农业科学院，北京100081

收稿日期:2007-02-02 修回日期:1900-01-01 出版日期:2007-11-12 网络出版日期:2007-11-12
通讯作者: 万建民

Mapping QTL for Rice Grain Weight across Different Environments

WU Xiu-Ju¹,WAN Xiang-Yuan¹,JIANG Ling¹,XIAO Ying-Hui¹,LIU Shi-Jia¹,CHEN Liang-Ming¹,ZHAI Hu-Qu³,WAN Jian-Min^12*

1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 3 Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2007-02-02 Revised:1900-01-01 Published:2007-11-12 Published online:2007-11-12
Contact: WAN Jian-Min

摘要/Abstract

摘要： 以粳稻Asominori为遗传背景的染色体片段置换系（CSSLs）群体为材料，利用基于性状-标记多元回归分析方法对稻谷粒重和精米粒重进行多环境的QTL定位。结果在5个环境共检测到6个粒重相关QTL，分布于第1、6、7和8染色体上，对表型变异的贡献率介于13%~35%；其中控制精米粒重的qMRW-1a和稻谷粒重的qPRW-1在不同环境中均能稳定表达，且均位于第1染色体RFLP标记XNpb113附近，该基因座还同时控制着粒宽。qMRW-1a和qPRW-1共同对应的置换系AIS8和AIS11与Asominori 的粒重差异在不同环境中均显著(P < 0.05)，表明该QTL的等位基因在不同环境中效应显著。比较发现该QTL在不同遗传群体中均能被重复检测到，且与蔗糖磷酸合酶基因（SPS）位置一致，推测该QTL与淀粉合成代谢有关。qMRW-1a 和qPRW-1在不同环境条件和遗传背景中表达，因此可用于进一步的精细定位研究。

关键词: 精米粒重, 稻谷粒重, 数量性状基因座（QTL）, 染色体片段置换系（CSSLs）

Abstract:

Grain weight is one of the important traits in rice for it determines grain yield and affects appearance quality in some degree. Grain weight is controlled by quantitative trait loci (QTLs) and determined by grain width, length, thickness and plumpness. To date, many QTLs have been identified, however only two QTLs have been fine mapped. It is urgent for identifying more QTLs expressed in different environments and genetic backgrounds so that they could be used in further fine mapping and breeding applications. So a chromosome segment substitution line (CSSL) population consisting of 65 lines, derived from Asominori and IR24 cross with the genetic background of Asominori, were grown in different environments to detect grain-weight related QTLs based on the stepwise regression of the traits and markers. The phenotypic values for milled rice weight (MRW) and brown rice weight ( BRW) showed a continuous distribution and some transgressive lines were also observed in the CSSLs population. A total of six QTLs for grain weight were detected in five environments, which located on the chromosomes 1, 6, 7, 8, and explained 13%–35% of phenotypic variance. The qMRW-1a and qPRW-1 could express stably in all experiment environments, both being in the same chromosome location of nearest marker XNpb113 on chromosome 1. Thus the markers could be used for marker assisted selection (MAS) of rice grain weight. MRW and PRW differed significantly between the CSSLs harboring the QTL allele and Asominori. In comparision with previous studies, they were found to be expressed in various genetic populations, and also had the similar chromosomal location with the gene for sucrose phosphate synthase. Therefore we deduced the QTL was associated with rice starch metabolism. qMRW-1a and qPRW-1, which had highly stable expression characteristic, should be used for fine mapping to elucidate the genetic basis of the rice grain weight.

Key words: Milled rice weight (MRW), Paddy rice weight (PRW), Quantitive trait loci (QTL), Chromosome segment substitution lines (CSSLs)

吴秀菊;万向元;江玲;肖应辉;刘世家;陈亮明;翟虎渠;万建民. 多环境下稻米粒重的QTL定位[J]. 作物学报, 2007, 33(11): 1771-1776.

WU Xiu-Ju;WAN Xiang-Yuan;JIANG Ling;XIAO Ying-Hui;LIU Shi-Jia;CHEN Liang-Ming;ZHAI Hu-Qu;WAN Jian-Min

. Mapping QTL for Rice Grain Weight across Different Environments[J]. Acta Agron Sin, 2007, 33(11): 1771-1776.

参考文献

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	李芳,程立锐,许美容,周政,张帆,孙勇,周永力,朱苓华,徐建龙,黎志康. 利用品质性状的回交选择导入系挖掘水稻抗纹枯病QTL[J]. 作物学报, 2009, 35(9): 1729-1737.
[2]	井文;江玲;张文伟;翟虎渠;万建民. 杂草稻种子休眠数量性状位点的定位[J]. 作物学报, 2008, 34(05): 737-742.
[3]	胡茂龙;张迎信;孔令娜;杨权海;王春明;翟虎渠;万建民. 水稻光合作用及相关生理性状的QTL分析[J]. 作物学报, 2007, 33(02): 183-188.
[4]	胡茂龙;张迎信;孔令娜;杨权海;王春明;翟虎渠;万建民. 利用回交重组自交系群体检测3个水稻光合功能相关性状QTL[J]. 作物学报, 2006, 32(11): 1630-1635.
[5]	张光恒;曾大力;胡时开;苏岩;阿加拉铁;郭龙彪;钱前. 水稻苗期耐淹相关性状QTL分析[J]. 作物学报, 2006, 32(09): 1280-1286.
[6]	陈静;江玲;王春明;池桥宏;翟虎渠;万建民. 籼粳杂种花粉不育基因的定位[J]. 作物学报, 2006, 32(04): 515-521.