Fan Lu" /> <SPAN class=datatitle>Leishman醋酸洋红C分带——一个新的改良的C分带方法</SPAN>
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作物学报

作物学报 ›› 1985, Vol. 11 ›› Issue (04): 245-248.

• 研究论文 • 上一篇    下一篇

Leishman醋酸洋红C分带——一个新的改良的C分带方法

樊路   

  1. 中国农业科学院作物育种栽培研究所
  • 收稿日期:1984-12-06 修回日期:1985-06-09 出版日期:1985-12-12 网络出版日期:1985-12-12

LEISHMAN ACETOCAMINE C-BANDING——A NEW IMPROVED METHOD OF C-BANDING

Fan Lu   

  1. Chinese Academy of Agricultural Sciences
  • Received:1984-12-06 Revised:1985-06-09 Published:1985-12-12 Published online:1985-12-12

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摘要： 本方法的特点是:在显带染色以前,用醋酸洋红染色。仅仅用盐酸处理玻片上的材料,而且在酒精处理以后,用低浓度的Leishman染色液染色而不用Giemsa或高浓度的Leishman染色液染色。 本方法的优点是:由于用醋酸洋红染色便于观察分带前染色体形态特征。不用盐酸处理根尖便于得到分离很好的染色体。本方法可以得到更多条带,而且带型

Abstract: The characteristics of this new improved method are as follows:Before Leishman stain banding, the root tips are stained with acetocam-ine. The slides are dipped in absolute alcohol and then treated with hy-drochloric acid. The chromosomes are stained with Leishman stain oflow concentration instead o

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