欢迎访问作物学报,今天是

作物学报 ›› 2006, Vol. 32 ›› Issue (04): 593-596.

• 研究论文 • 上一篇    下一篇

蜂毒肽对农作物生理指标及防御系统酶影响的研究

王关林;邢卓;潘凌子;方宏筠   

  1. 辽宁师范大学生命科学学院,辽宁大连 116029
  • 收稿日期:2005-02-01 修回日期:1900-01-01 出版日期:2006-04-12 网络出版日期:2006-04-12
  • 通讯作者: 王关林

Effects of Melittin on the Physiological Indices and Defensive Enzymes in Crops

WANG Guan-Lin; XING Zhuo; PAN Ling-Zi and FANG Hong-Jun   

  1. Life Science Department of Liaoning Normal University, Dalian 116029, Liaoning, China
  • Received:2005-02-01 Revised:1900-01-01 Published:2006-04-12 Published online:2006-04-12
  • Contact: WANG Guan-Lin

摘要:

系统研究了蜂毒肽对玉米、番茄、大豆、大白菜等农作物生长发育过程中生理指标的影响及对玉米防御系统酶的作用。结果表明,蜂毒肽对农作物的影响具有普遍性,对细胞中的叶绿素、可溶性糖、核酸及蛋白质含量,低浓度有一定促进作用,高浓度有抑制作用,但影响都很微弱(t检验P>0.05)。蜂毒肽对植物防御系统酶具有诱导效应,5 mg/L蜂毒肽使其活性快速增加,其中PAL、PPO在喷药后48 h达到峰值,分别比对照提高178%和9.8%;而SOD在6 h即可达到峰值,比对照提高31.5%;只有POD在喷洒50 mg/L蜂毒肽后48 h达到最大值。玉米POD同工酶谱的研究进一步表明蜂毒肽对有的酶谱带(品种1的P11谱带)有很强的诱导作用,有的甚至诱导出1条新带(品种2的P4谱带);而对有的谱带有抑制作用,而且对不同品种POD酶的作用不尽相同,但喷药后2个品种POD酶总体含量均高于对照,与酶活测定结果相同。

关键词: 蜂毒肽, 农作物, 生理指标, 防御系统酶, 同工酶酶谱

Abstract:

The content levels of chlorophyll, soluble sugar, nucleic acid and protein of crops were promoted by the low concentration(5 mg/L) of melittin, and inhibited by the higher (50 mg/L) one, although the effects were not significant(t-test, P>0.05)in four crops. Their PAL and PPO activities were reached the maximal levels 48 hours after spraying the solution of melittin (5 mg/L), which were increased by 178% and 9.8% respectively as compared with the control, while the activity of SOD was increased by 31.5% after 6 hours. The activity of POD was reached the maximal levels under the treatment of 50mg/L melittin. The land pattern of POD isoenzyme in maize elucidated further that there were strong induction to some zymogram bands(egP11)while inhibition to others, and different effects on the different cultivars showing the particularity of cultivars.

Key words: Melittin, Crops, Physiological indices, Defensive enzyme, Zymogram of isoenzyme

中图分类号: 

  • Q945
[1] 王洋洋, 贺利, 任德超, 段剑钊, 胡新, 刘万代, 郭天财, 王永华, 冯伟. 基于主成分-聚类分析的不同水分冬小麦晚霜冻害评价[J]. 作物学报, 2022, 48(2): 448-462.
[2] 李晓旭, 王蕊, 张利霞, 宋亚萌, 田晓楠, 葛荣朝. 水稻基因OsATS的克隆及功能鉴定[J]. 作物学报, 2021, 47(10): 2045-2052.
[3] 李晶岚,陈鑫欣,石翠翠,刘方惠,孙静,葛荣朝. OsRPK1基因过表达和RNA干涉对水稻苗期耐盐性的影响[J]. 作物学报, 2020, 46(8): 1217-1224.
[4] 李智,李国龙,张永丰,于超,苏文斌,樊福义,张少英. 膜下滴灌条件下高产甜菜灌溉的生理指标[J]. 作物学报, 2017, 43(11): 1724-1730.
[5] 魏颖娟,赵杨,邹应斌*. 不同穗型超级稻品种籽粒灌浆特性[J]. 作物学报, 2016, 42(10): 1516-1529.
[6] 贺晓岚,王建伟,李文旭,陈真真,赵继新,武军,王中华,陈新宏. 大赖草6-SFT基因的克隆及其转基因烟草抗旱和抗寒性分析[J]. 作物学报, 2016, 42(03): 389-398.
[7] 陈丽娜,方沩,司海平,曹永生. 农作物种质资源本体构建研究[J]. 作物学报, 2016, 42(03): 407-414.
[8] 王小丽,杜建中,郝曜山,张丽君,赵欣梅,王亦学,孙毅. BADH基因玉米植株的获得及其耐盐性分析[J]. 作物学报, 2014, 40(11): 1973-1979.
[9] 王后苗, 廖伯寿. 农作物收获前黄曲霉毒素污染与控制措施[J]. 作物学报, 2012, 38(01): 1-9.
[10] 张娟; 张正斌;谢惠民;董宝娣; 胡梦芸;徐萍. 小麦叶片水分利用效率及相关生理性状的关系研究[J]. 作物学报, 2005, 31(12): 1593-1599.
[11] 张治礼;郑学勤. 内源细胞分裂素调控油菜叶片衰老进程的研究[J]. 作物学报, 2005, 31(01): 1-6.
[12] 冯固;灯莎;茂秋;晓林;福锁;生秀. 盐胁迫下AM真菌对玉米生长及耐盐生理指标的影响[J]. 作物学报, 2000, 26(06): 743-750.
[13] 曹永生;张贤珍;白建军;龚高法. 中国主要粮食作物野生种质资源地理分布[J]. 作物学报, 1999, 25(04): 424-432.
[14] 高明君;陈家驹. 栽培粟起源的同工酶研究[J]. 作物学报, 1988, 14(02): 131-136.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!