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作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (7): 1173-1180.doi: 10.3724/SP.J.1006.2009.01173

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

水稻抗白叶枯病新基因Xa32（t）的鉴定和初步定位

郑崇珂1,2,**，王春连2,3,**，于元杰1，梁云涛2，赵开军2,3,*   

  1. 1山东农业大学农科院/作物生物学高家重点实验室，山东泰安271018；2中国农业科学院作物科学研究所/农业部作物遗传育种重点实验室，北京100081；3农作物基因资源与基因改良高家重大科学工程，北京100081
  • 收稿日期:2009-01-21 修回日期:2009-03-13 出版日期:2009-07-12 网络出版日期:2009-05-18
  • 通讯作者: 赵开军，E-mail:zhaokj@mail.caas.net.cn
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）项目（2006AA10Z106）和中央级公益性科研院所基金项目（2060302-2-08）资助。

Identification and Molecular Mapping of Xa32(t),a Novel Resistance Gene for Bacterial Blight(Xanthomonas oryzae pv.oryzae)in Rice

ZHENG Chong-Ke1,2,**,WANG Chun-Lian2,3,**, YU Yuan-Jie1, LIANG Yun-Tao2,ZHAO Kai-Jun23*   

  1. 1College of Agronomy,Shandong Agricultural University/State Key Laboratory of Crop Biology,Tai'an 271018,China;2Key Laboratory of Crop Genetics and Breeding,Ministry of Agriculture/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,China
  • Received:2009-01-21 Revised:2009-03-13 Published:2009-07-12 Published online:2009-05-18
  • Contact: ZHAO Kai-Jun，E-mail:zhaokj@mail.caas.net.cn

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被引次数

29

摘要：

通过多菌系接种鉴定及抗谱分析，并与目前国际上已知抗白叶枯病基因比较，证明在水稻抗源C4064中含有一个新的抗白叶枯病基因，暂命名为Xa32(t)。应用分离集团分析法(BSA)，借助SSR和EST等分子标记，对该基因进行了分子标记定位。通过对F2分离群体及F3家系单株进行遗传连锁性检测，发现6个位于水稻第11染色体长臂末端的分子标记RM27256、RM27274、RM2064、ZCK24、RM6293和RM5926与Xa32(t)基因连锁。它们与Xa32(t)基因间的遗传距离分别为2.1、1.0、1.0、0.5、1.5和2.6 cM。其中标记RM6293和RM5926位于染色体近端粒一侧，其他4个标记RM27256、RM27274、RM2064和ZCK24位于基因的另一侧。将Xa32(t)定位在水稻第11染色体长臂末端2.0 cM范围内。

关键词: 水稻白叶枯病, 抗谱分析, Xa32(t), 放在标记定位

Abstract:

The rice bacterial blight (BB), caused by Xanthmonas oryzae pv. oryzae (Xoo),is the most devastating bacterial disease of rice worldwide. Use of resistant varieties has been thought the most economical and environment-friendly approach to control BB of rice. Since the rapid changes of the pathogenicity of the pathogen (Xoo), new BB resistance genes are always needed for rice breeding. We, here, report the identification and molecular mapping of a new BB resistance gene from Oryzae ustraliensis. Based on the resistance spectrum analysis, we identified a new rice germplasm, C4064, for bacterial blight (BB) resistance. Leaf-cutting innoculation assays showed that C4064 was resistant to Xoo strains P1 (PXO61), P4 (PXO71), P5 (PXO112), P6 (PXO99), P7 (PXO145), P8 (PXO280),P9 (PXO339), and KX085, but susceptible to P2 (PXO86) and P3 (PXO79). Comparison of resistance spectrum with that of all the known BB resistance genes and genetic analysis revealed that the rice germplasm C4064harbored a new BB-resistance gene, designated as Xa32(t). To molecularly map Xa32(t) gene,Bulked Segregant Analysis (BSA) was adopted to survey SSR and EST molecular markers. Out of 299 markers tested, six markers RM27256, RM27274, RM2064, ZCK24, RM6293, and RM5926 on rice chromosome 11 displayed polymorphism between the S-pool and R-pool. By analyzing the F2 and F3 populations, the gene Xa32 (t) was mapped on the long arm of rice chromosome 11. Linkage analysis revealed that molecular markers RM27256, RM27274, RM2064, and ZCK24 were located between Xa32 (t) and the centromere of the chromosome, with genetic distances of 2.1, 1.0, 1.0, and 0.5 cM to Xa32 (t), respectively; while RM6293 and RM5926 were located onthe othersideof Xa32(t), with genetic distances of 1.5 and 2.6 cM to Xa32 (t), respectively. Thus, the new BB-resistance gene Xa32(t) was mapped within a length of 2.0 cM on the long arm of rice chromosome 11. The results of this study will be useful in fine mapping of Xa32(t) and marker-assisted breeding for BB resistant rice varieties.

Key words: Rice bacterial blight, Resistance spectrum analysis, Xa32(t), Molecular mapping

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