作物学报 ›› 2009, Vol. 35 ›› Issue (7): 1173-1180.doi: 10.3724/SP.J.1006.2009.01173
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
郑崇珂1,2,**,王春连2,3,**,于元杰1,梁云涛2,赵开军2,3,*
ZHENG Chong-Ke1,2,**,WANG Chun-Lian2,3,**, YU Yuan-Jie1, LIANG Yun-Tao2,ZHAO Kai-Jun23*
摘要:
通过多菌系接种鉴定及抗谱分析,并与目前国际上已知抗白叶枯病基因比较,证明在水稻抗源C4064中含有一个新的抗白叶枯病基因,暂命名为Xa32(t)。应用分离集团分析法(BSA),借助SSR和EST等分子标记,对该基因进行了分子标记定位。通过对F2分离群体及F3家系单株进行遗传连锁性检测,发现6个位于水稻第11染色体长臂末端的分子标记RM27256、RM27274、RM2064、ZCK24、RM6293和RM5926与Xa32(t)基因连锁。它们与Xa32(t)基因间的遗传距离分别为2.1、1.0、1.0、0.5、1.5和2.6 cM。其中标记RM6293和RM5926位于染色体近端粒一侧,其他4个标记RM27256、RM27274、RM2064和ZCK24位于基因的另一侧。将Xa32(t)定位在水稻第11染色体长臂末端2.0 cM范围内。
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