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作物学报 ›› 2010, Vol. 36 ›› Issue (10): 1649-1656.doi: 10.3724/SP.J.1006.2010.01649

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

中国小麦微核心种质及地方品种籽粒休眠特性的分子标记鉴定

张海萍1,常成1,*,游光霞2,张秀英2,闫长生2,肖世和2,司红起1,卢杰1,马传喜1,*   

  1. 1安徽农业大学农学院/农业部小麦区域技术创新中心/安徽省小麦技术产业工程研究中心，安徽合肥230036；2中国农业科学院作物科学研究所/国家小麦改良中心/国家农作物基因资源与基因改良重大科学工程，北京100081
  • 收稿日期:2010-03-19 修回日期:2010-05-29 出版日期:2010-10-12 网络出版日期:2010-08-04
  • 通讯作者: 常成, E-mail: changtgw@126.com; 马传喜, E-mail: machuanxi@yahoo.cn
  • 基金资助:

    本研究由国家科技支撑计划项目（2006BAD01A02），农业部公益性行业科研专项（nyhyzx07-002),安徽省自然科学研究项目（KJ2008B211），农业部农业结构调整重大技术专项（06-02-03B）和安徽农业大学青年基金重点项目资助。

Identification of Molecular Markers Associated with Seed Dormancy in Mini Core Collections of Chinese Wheat and Landraces

ZHANG Hai-Ping1,CHANG Cheng1*,YU Guang-Xia2,ZHANG Xiu-Ying2,YAN Chang-Sheng2,XIAO Shi-He2,SI Hong-Qi1,LU Jie1,MA Chuan-Xi1*   

  • Received:2010-03-19 Revised:2010-05-29 Published:2010-10-12 Published online:2010-08-04
  • Contact: CHANG Cheng,E-mail:changtgw@126.com;MA Chuan-xin,E-mail:machuanxi@yahoo.cn

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1694

被引次数

28

摘要： 为探索我国小麦微核心种质及地方品种籽粒休眠的遗传基础，利用已报道的4个3AS上的SSR标记(Xbarc57、Xbarc294、Xbarc310和Xbarc321)和1个3BL上的Viviparous-1基因标记Vp1-b2对107份我国小麦微核心种质及31份地方品种进行籽粒休眠的分子标记鉴定。结果表明，5个分子标记在试验材料中表现出丰富的等位变异，具有5~6种等位类型，与籽粒萌芽指数(GI)密切相关。根据一般线性模型分析结果，各位点的等位变异显著影响籽粒休眠，其中Vp1-b2和Xbarc294对籽粒休眠作用较其他标记大，可分别解释65.8%和61.2% 的表型变异；其次是Xbarc310 (56.3%)和Xbarc57 (55.8%)，最小的是Xbarc294(53.3%)。而5个标记联合可解释95.9% 的性状变异，其次是Vp1-b2和Xbarc294的组合(89.1%)，解释变异最小的标记组合是 Vp1-b2和Xbarc321 (79.4%)。5个分子标记即可解释籽粒休眠的绝大部分表型变异，说明我国小麦微核心种质及地方品种籽粒休眠特性受3AS和3BL上的2个主效基因控制。

Abstract: Seed dormancy evaluated by germination index (GI) is often regarded as a main and pivotal component of observed genetic variation for pre-harvest sprouting (PHS). Improving seed dormancy can decrease or avoid PHS damage to wheat (Triticum aestivum L.) before harvest, but it is a complicated trait controlled by multi-genes and influenced by many environmental factors. Because seed dormancy is difficult to be accurately evaluated under field condition, molecular markers will play an important role in dormancy evaluation. In this study, four SSR markers (Xbarc57, Xbarc294, Xbarc310, and Xbarc321) on the short arm of chromosome 3A and a gene-based marker (Vp1-b2) derived from Vp-1B on 3BL were used for genotyping 138 mini core collections of Chinese wheat and landraces. The results indicated that rich alleles occurred in the five markers, and most were significantly correlated with GI value. Based on general linear model, the five markers were significantly associated with seed dormancy. Markers Vp1-b2 and Xbarc294 had stronger effects on seed dormancy than other markers, accounting for 65.8% and 61.2% of seed dormancy variation, respectively. Marker combination could promote the percentage of phenotypic variation explained, of which the combination of five markers estimated 95.9% of the GI variation, followed by the combination of Vp1-b2 and Xbarc294 (89.1%), and the combination of Vp1-b2 and Xbarc321 had the smallest estimation of GI variation (79.4%). These results indicate that seed dormancy is mainly attributed to loci Vp1-b2 and Xbarc294 on 3AS and 3BL in the 138 genotypes, and the evaluation accuracy can be enhanced by jointly application of the total five markers in markers-assisted selection.

Key words: Triticum aestivum L., Seed dormancy, Molecular marker, Mini core collections of Chinese wheat

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