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作物学报 ›› 2010, Vol. 36 ›› Issue (10): 1642-1648.doi: 10.3724/SP.J.1006.2010.01642

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用SRAP标记构建18个木薯品种的DNA指纹图谱

齐兰1,2,王文泉3,张振文1,叶剑秋1,李开绵1,*   

  1. 1 中国热带农业科学院热带作物品种资源研究所，海南儋州 571737；2 海南大学农学院，海南儋州 571737；3 中国热带农业科学院热带生物技术研究所，海南海口 571101
  • 收稿日期:2010-03-23 修回日期:2010-05-30 出版日期:2010-10-12 网络出版日期:2010-08-04
  • 通讯作者: 李开绵, E-mail: likaimian@sohu.com
  • 基金资助:

    本研究受国家木薯现代农业产业技术体系项目（nycytx-17)，国家公益性行业科研专项（3-57），国家重点技术研究发展计划（973计划）项目（2010CB126606）和中国农业科学院品种资源研究所研究生科研基金项目（YJS-2008-S009）的资助。

DNA Fingerprinting Analysis of 18 Cassava Varieties Using Sequence-Related Amplified Polymorphism Markers

JI  Lan1,2,WANG Wen-Quan3,ZHANG Zhen-Wen1,YE Jian-Qiu1,LI  Kai-Mian1*   

  1. 1 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China; 2 Hainan University, Danzhou 571737, China; 3 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
  • Received:2010-03-23 Revised:2010-05-30 Published:2010-10-12 Published online:2010-08-04
  • Contact: LI Kai-Mian,E-mail:likaimian@sohu.com

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被引次数

82

摘要： 利用SRAP标记，选用36对多态性较好的引物组合，对18个木薯品种进行分析鉴定，扩增出320个位点，其中多态性位点235个，多态性比率达73.4%，平均每对引物组合可产生8.9个位点和6.5个多态性位点；235个多态性位点采用非加权组平均法(UPGMA)进行聚类分析，以遗传相似系数0.734为阈值，可将18份供试材料分为4组；选用2对多态性引物Me1-Em5和Me24-Em10，初步构建了18份木薯品种的指纹图谱，根据条带的有无转换为0、1二进制编码形成数字指纹，每个品种拥有唯一的数字指纹区别于其他品种，置信概率达到99.999%。结果表明，采用SRAP标记建立的指纹图谱适用于木薯品种的分类和鉴定。

关键词: 木薯, SRAP, DNA指纹图谱

Abstract: There widely exists a case of same name for more varieties or different names for one variety in cassava agriculture production, so it is difficult to identifycassava varieties on the basis of the phenotypic characters. In the paper, SRAP marker was applied to detect 18 cassava varieties, thirty-six primer combinations were selected with abundant polymorphism, a total of 320 bands were scored, 235 (73.4%) out of them were polymorphic, with an average of 8.9 bands and 6.5 polymorphic bands for each primer combination, 235 polymorphic bands then were used to develop a dendrogram with Unweighted Pair-Group Method Arithmetic Average (UPGMA), and 18 cassava varieties were divided into four major groups at the 0.734 similarity level; two primer combinations Me1-Em5 and Me24-Em10, were used to develop the DNA fingerprints for the 18 cassava varieties. The DNA fingerprints were then converted into binary codes, with 1 and 0 representing band presence and absence, respectively. In the DNA fingerprints, each of the 18 cassava varieties had its unique binary code, which distinguished varieties between each other easily, with 99.999% probability of confidence. The results demonstrated that it is feasible to distinguish varieties with DNA fingerprints established by SRAP.

Key words: Cassava, SRAP, DNA fingerprint

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