作物学报 ›› 2010, Vol. 36 ›› Issue (10): 1642-1648.doi: 10.3724/SP.J.1006.2010.01642
齐兰1,2,王文泉3,张振文1,叶剑秋1,李开绵1,*
JI Lan1,2,WANG Wen-Quan3,ZHANG Zhen-Wen1,YE Jian-Qiu1,LI Kai-Mian1*
摘要: 利用SRAP标记,选用36对多态性较好的引物组合,对18个木薯品种进行分析鉴定,扩增出320个位点,其中多态性位点235个,多态性比率达73.4%,平均每对引物组合可产生8.9个位点和6.5个多态性位点;235个多态性位点采用非加权组平均法(UPGMA)进行聚类分析,以遗传相似系数0.734为阈值,可将18份供试材料分为4组;选用2对多态性引物Me1-Em5和Me24-Em10,初步构建了18份木薯品种的指纹图谱,根据条带的有无转换为0、1二进制编码形成数字指纹,每个品种拥有唯一的数字指纹区别于其他品种,置信概率达到99.999%。结果表明,采用SRAP标记建立的指纹图谱适用于木薯品种的分类和鉴定。
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