瓜尔豆发育过程中碳水化合物含量的变化

作物学报 ›› 2007, Vol. 33 ›› Issue (11): 1869-1873.

瓜尔豆发育过程中碳水化合物含量的变化

胡春梅^1,2;王秀峰^2,*;季俊杰³;朱月林¹

1 南京农业大学园艺学院，江苏南京210095；2 山东农业大学园艺学院，山东泰安271018；3 南京农业大学图书馆，江苏南京210095

收稿日期:2006-12-04 修回日期:1900-01-01 出版日期:2007-11-12 网络出版日期:2007-11-12
通讯作者: 王秀峰

Changes in Carbohydrate Contents during Guar Plant Development

HU Chun-Mei¹²,WANG Xiu-Feng^2*,JI Jun-Jie³,ZHU Yue-Lin¹

1 College of Horticulture, Nanjing Agricultural University, Nanjing 210095, Jiangsu; 2 College of Horticulture, Shandong Agricultural University, Tai’an 271018, Shandong; 3 Library, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China

Received:2006-12-04 Revised:1900-01-01 Published:2007-11-12 Published online:2007-11-12
Contact: WANG Xiu-Feng

摘要/Abstract

摘要：

研究了瓜尔豆生长发育过程中叶片、种荚及籽粒中蔗糖合成酶（SS）和蔗糖磷酸合成酶（SPS）活性的变化、糖类物质的组成及其含量的动态变化，以揭示可溶性糖的合成对瓜尔豆胚乳中半乳甘露聚糖和淀粉积累的影响。结果表明，进入开花期（播种后45 d）后，叶片、种荚和籽粒中SS和SPS活性均呈降低趋势。籽粒中的SS和SPS活性呈正相关，且显著高于叶片和种荚中相应酶活性。叶片中葡萄糖（Glc）含量高于蔗糖（Suc）。种荚中可溶性糖含量与Suc含量均呈单峰曲线，在籽粒直径5 mm时达峰值，两者呈正相关。Suc是种荚中暂存含量最多的可溶性糖，但其含量与SS和SPS活性无显著相关性，它主要来自叶片。种荚中Suc含量与籽粒碳水化合物总量（可溶性糖+多糖）呈负相关，它是籽粒碳水化合物合成的重要物质来源，还与籽粒库的建成有关。随种子发育，籽粒可溶性糖含量呈逐步下降趋势。籽粒发育前期，叶片同化物以Suc的形式运输并大量积累在种荚中，当籽粒直径达5 mm后迅速由种荚卸载至籽粒。但卸载期籽粒Suc含量较低，且显著低于种荚，说明籽粒Suc迅速转化为其他可溶性糖，并进一步用于多糖合成。籽粒直径为4 mm至5 mm时是种荚由“源”到“库”转换的临界期，这时籽粒中淀粉积累较迅速，半乳甘露聚糖在籽粒直径达5 mm后进入迅速积累期。

关键词: 瓜尔豆, 蔗糖, 可溶性糖, 半乳甘露聚糖, 蔗糖合成酶, 蔗糖磷酸合成酶

Abstract:

Guar (Cyamposis Tetragonoloba (L.) Taub.) is an important raw material for guar gum, in which galactomannan (GM) is the active component. GM is a main storage carbohydrate in guar seeds, which is an analogue to that of starch in cereal grains. Sucrose is a common precursor for the biosynthesis of GM and starch. Studies were made on the changes in sucrose synthetic enzymes and carbohydrate contents in guar plants, to understand the effect of soluble sugar biosynthesis on accumulation of endospermic polysaccharides in this special plant. Sucrose synthetase (SS) and sucrose phosphate synthetase (SPS) activities, compositions and contents of carbohydrates in guar leaves, seeds and pod peels were determined. The results showed that SS and SPS activities in leaves, pod peels and seeds decreased with the development of seeds. SS and SPS activities in the seeds were higher than those in leaves and pod peels. SS activity showed a positive correlation with SPS activity in seeds. Glucose content was significantly higher than sucrose content in leaves. Contents of soluble sugar and sucrose increased in pod peels, till the diameter of seed was about 5 mm, and then decreased quickly, they were well correlated with each other. Soluble sugar of seeds decreased gradually during seed development. Sucrose content in pod peels showed a negative correlation with total carbohydrate contents (soluble sugar + polysaccharides) in seeds, and it is an important source of the carbohydrate in the seeds. It took part in the carbohydrate synthesis and also the establishment of seed sink. Carbohydrates in the leaves, as sucrose transported to and temporarily deposited in the pod peels in the early period of seed development, and then transported rapidly from pod peels into seeds at the seed diameter of about 5 mm. However, there was less sucrose in seeds than in pod peels, it suggested that sucrose was inverted into other reduced sugars after transported into seeds. Starch accumulated quickly in the seeds when the seed diameter was about 4 to 5 mm, a critical period for pod peels changing from ‘sink’ to ‘source’, and galactomannan increased dramatically in the seeds after that.

Key words: Cyamposis tetragonoloba (L.) Taub., Sucrose, Soluble sugar, Galactomannan, Sucrose synthetase, Sucrose phosphate synthetase

胡春梅;王秀峰;季俊杰;朱月林. 瓜尔豆发育过程中碳水化合物含量的变化[J]. 作物学报, 2007, 33(11): 1869-1873.

HU Chun-Mei;WANG Xiu-Feng;JI Jun-Jie;ZHU Yue-Lin. Changes in Carbohydrate Contents during Guar Plant Development[J]. Acta Agron Sin, 2007, 33(11): 1869-1873.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/

https://zwxb.chinacrops.org/CN/Y2007/V33/I11/1869

参考文献

相关文章 15

[1]	袁鑫, 赵卓凡, 赵瑞清, 刘孝伟, 郑名敏, 刘育生, 董好胜, 邓丽娟, 曹墨菊, 黄强. 一份玉米小籽粒发育突变体mn-like1的遗传分析与分子鉴定[J]. 作物学报, 2025, 51(6): 1569-1581.
[2]	郭建斌, 原小燕, 符明联, 陈伟刚, 罗怀勇, 刘念, 黄莉, 周小静, 姜慧芳, 廖伯寿, 雷永. 不同海拔生态区花生含油量和蔗糖含量的差异及蔗糖含量关联分析[J]. 作物学报, 2025, 51(11): 3005-3012.
[3]	郅晨阳, 薛晓梦, 吴洁, 李雄才, 王瑾, 晏立英, 王欣, 陈玉宁, 康彦平, 王志慧, 淮东欣, 洪彦彬, 姜慧芳, 雷永, 廖伯寿. 花生籽仁蔗糖含量遗传模型分析[J]. 作物学报, 2024, 50(1): 32-41.
[4]	胡美玲, 郅晨阳, 薛晓梦, 吴洁, 王瑾, 晏立英, 王欣, 陈玉宁, 康彦平, 王志慧, 淮东欣, 姜慧芳, 雷永, 廖伯寿. 单粒花生蔗糖含量近红外预测模型的建立[J]. 作物学报, 2023, 49(9): 2498-2504.
[5]	高春华, 冯波, 李国芳, 李宗新, 李升东, 曹芳, 慈文亮, 赵海军. 施氮量对花后高温胁迫下冬小麦籽粒淀粉合成的影响[J]. 作物学报, 2023, 49(3): 821-832.
[6]	郭建斌, 成良强, 李威涛, 刘念, 罗怀勇, 丁膺宾, 喻博伦, 陈伟刚, 黄莉, 周小静, 雷永, 廖伯寿, 姜慧芳. 花生蔗糖含量与蛋白质和含油量的相关性分析及蔗糖含量QTL定位[J]. 作物学报, 2023, 49(10): 2698-2704.
[7]	吴旭莉, 吴正丹, 晚传芳, 杜叶, 高艳, 李賾萱, 王志前, 唐道彬, 王季春, 张凯. 甘薯糖转运蛋白IbSWEET15的功能研究[J]. 作物学报, 2023, 49(1): 129-139.
[8]	曹际玲, 曾青, 朱建国. 不同品种小麦灌浆期旗叶光合特性及光合基因表达对臭氧浓度升高的响应[J]. 作物学报, 2022, 48(9): 2339-2350.
[9]	胡美玲, 薛晓梦, 吴洁, 郅晨阳, 刘念, 陈小平, 王瑾, 晏立英, 王欣, 陈玉宁, 康彦平, 王志慧, 淮东欣, 姜慧芳, 雷永, 廖伯寿. 花生籽仁脂肪和蔗糖含量的胚、细胞质、母体遗传效应分析[J]. 作物学报, 2022, 48(11): 2724-2732.
[10]	宋天晓, 刘意, 饶莉萍, Soviguidi Deka Reine Judesse, 朱国鹏, 杨新笋. 甘薯细胞壁蔗糖转化酶基因IbCWIN家族成员鉴定及表达分析[J]. 作物学报, 2021, 47(7): 1297-1308.
[11]	唐婧泉, 王南, 高界, 刘婷婷, 文静, 易斌, 涂金星, 傅廷栋, 沈金雄. 甘蓝型油菜SnRK基因家族生物信息学分析及其与种子含油量的关系[J]. 作物学报, 2021, 47(3): 416-426.
[12]	雷永, 王志慧, 淮东欣, 高华援, 晏立英, 李建国, 李威涛, 陈玉宁, 康彦平, 刘海龙, 王欣, 薛晓梦, 姜慧芳, 廖伯寿. 花生籽仁蔗糖含量近红外模型构建及在高糖品种培育中的应用[J]. 作物学报, 2021, 47(2): 332-341.
[13]	李威涛, 郭建斌, 喻博伦, 徐思亮, 陈海文, 吴贝, 龚廷锋, 黄莉, 罗怀勇, 陈玉宁, 周小静, 刘念, 陈伟刚, 姜慧芳. 基于HPLC-RID的花生籽仁可溶性糖含量检测方法的建立[J]. 作物学报, 2021, 47(2): 368-375.
[14]	李瑞杰,唐会会,王庆燕,许艳丽,王琦,卢霖,闫鹏,董志强,张凤路. 5-氨基乙酰丙酸和乙烯利对东北春玉米源库碳平衡的调控效应[J]. 作物学报, 2020, 46(7): 1063-1075.
[15]	王丹丹, 柳洪鹃, 王红霞, 张鹏, 史春余. 甘薯蔗糖转运蛋白基因IbSUT3的克隆及功能分析[J]. 作物学报, 2020, 46(7): 1120-1127.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed