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作物学报 ›› 2011, Vol. 37 ›› Issue (06): 1116-1123.doi: 10.3724/SP.J.1006.2011.01116

• 研究简报 • 上一篇    

应用ISSR分子标记绘制红麻种质资源DNA指纹图谱

汪斌1,祁伟1,**,兰涛1,陈惠端2,徐建堂1,粟建光3,李爱青4,祁建民1,*   

  1. 1福建农林大学/作物遗传育种与综合利用教育部重点实验室, 福建福州 350002; 2 福建农林大学机电工程学院, 福建福州 350002; 3 中国农业科学院麻类研究所, 湖南长沙 410205; 4 安徽省种子管理总站, 安徽合肥 230001
  • 收稿日期:2010-09-16 修回日期:2011-03-06 出版日期:2011-06-12 网络出版日期:2011-03-24
  • 通讯作者: 祁建民, E-mail: qijm863@163.com, Tel: 0591-83644898
  • 基金资助:

    本研究由福建省科技重点项目(2008N0005), 福建省科技重点项目(2007N0011), 国家麻类产业技术体系建设(nycytx-19-s12), 国家公益性行业计划(nyhyzx07-018-3)项目资助。

Establishment of DNA Fingerprintings of Kenaf (Hibiscus Cannabinus L.) Germplasm Resources with ISSR Molecular Markers

WANG Bin1,**,QI Wei1,**,LAN Tao1,CHEN Hui-Duan2,XU Jian-Tang1,SU Jian-Guang3,LI Ai-Qing4,QI Jian-Ming1,*   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 College of Mechanical and Electrical Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China; 4 Anhui Province Seeds Management Station, Hefei 230001, China
  • Received:2010-09-16 Revised:2011-03-06 Published:2011-06-12 Published online:2011-03-24
  • Contact: 祁建民, E-mail: qijm863@163.com, Tel: 0591-83644898

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被引次数

72

摘要： 以6份红麻种质资源为材料, 对UBC807~UBC80等80个ISSR引物进行筛选, 筛选出多态性好的ISSR引物20个。利用这20个ISSR引物扩增来自国内外84份红麻种质资源, 共获得230条谱带, 平均每个引物扩增出11.5条谱带, 其中多态性谱带185条, 多态性条带比率为80.43%, 表明供试的红麻种质资源遗传多样性较丰富。以供试84份红麻种质资源的ISSR扩增谱带为基础, 建立了供试材料扩增条带指纹数据库的Excel文件。根据指纹图谱唯一性原则, 采用自行开发的DNA指纹数据分析软件, 再从20个多态性好的ISSR引物中遴选出UBC 813、UBC 825、UBC 836、UBC 888和UBC 889引物, 绘制出82个红麻种质资源的DNA指纹图谱, 为红麻种质资源分子身份证的构建奠定了基础。

关键词: 红麻, 种质资源, DNA, ISSR, 指纹图谱

Abstract: Kenaf (Hibiscus cannabinus L.) is an important economic crop in China. To identify germplasm resources and establish the data base of DNA fingerprintings, we adopted six kenaf germplasm resources to screen 80 ISSR primers, 20 of which were polymorphic. Then 20 ISSR primers were used to amplify 84 kenaf germplasm resources introduced and preserved from home and abroad. Totally 230 bands were produced,the average number of DNA bands amplified by each primer was 11.5, and the number of polymorphic DNA bands was 185. The polymorphic proportion of DNA bands was 80.43%, which indicated the abundant genetic diversity of kenaf germplasm resources preserved. Based on the DNA bands amplified from 84 kenaf germplasm resources, the Excel data base for PCR amplified bands of the kenaf germplasm resources was established. According to the principle of uniqueness of the fingerprintings, the DNA fingerprints of 82 kenaf germplasmwere constructed with five ISSR primers (UBC813, UBC825, UBC836, UBC888 and UBC889) selected from 20 polymorphic primers, using the analyzing software designed by our laboratory programmed and based on image manipulation. The fingerprintings provide a basis of the molecular identification of kenaf germplasm resources. In this research, the DNA fingerprintings system was proved to be feasible and reliable.

Key words: Kenaf (Hibiscus cannabinus L.), Germplasm resource, DNA, ISSR, Fingerprinting

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