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作物学报 ›› 2011, Vol. 37 ›› Issue (11): 1975-1983.doi: 10.3724/SP.J.1006.2011.01975

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

142份甜高粱品种的分子身份证构建

王黎明，焦少杰，姜艳喜，严洪冬，苏德峰，孙广全   

  1. 黑龙江省农业科学院作物育种研究所，黑龙江哈尔滨 150086
  • 收稿日期:2011-05-25 修回日期:2011-07-15 出版日期:2011-11-12 网络出版日期:2011-09-06
  • 基金资助:

    本研究由现代农业产业技术体系专项资金(nycytx-12-03-02)和黑龙江省科技攻关项目(GA09B101-5-12)项目资助
    第一作者联系方式: E-mail:dawnw@126.com

Establishment of Molecular Identity in 142 Sweet Sorghum Varieties

WANG Li-Ming,JIAO Shao-Jie,JIANG Yan-Xi,YAN Hong-Dong,SU De-Feng,SUN Guang-Quan   

  1. Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2011-05-25 Revised:2011-07-15 Published:2011-11-12 Published online:2011-09-06

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1406

被引次数

57

摘要： 从分布在高粱染色体10个连锁群上的103对SSR引物中，筛选出41对多态性引物, 并用其扩增国内外142份甜高粱种质资源, 检测到189个多态性片段, 每对引物的等位基因数在2~11之间，平均为4.6个。引物位点的多态信息含量指数(PIC)变幅为0.089~0.850，平均为0.543。品种间特异指数差异较大，介于109.1~454.7之间，平均为189.0。结果表明, 根据引物的等位基因数确定11对引物(Xtxp329、Xtxp258、Xtxp113、Xtxp303、Xtxp61、Xtxp201、Xtxp14、Xtxp91、Xtxp47、Xtxp217和Xtxp67)组合, 并用于构建142份品种资源的分子身份证, 有效地区分了各品种。

关键词: 甜高粱, 种质资源, 分子身份证

Abstract: A large change occurs in phenotypic characters under different environments for introducing and frequently exchanging of abundant varieties, and accurate identification is usually affected by the similar phenotypic characters performed in the field, so it is necessary to establish ID to distinguish varieties between each other. A total of 103 SSR primers covering 10 linkage groups of sorghum chromosome were screened, 41 primers showing polymorphic bands were used to establish molecular ID of 142 sweet sorghum germplasm resources. A total of 189 polymorphic bands were amplified and obtained. The number of alleles revealed by each primer was 2-11 with an average of 4.6 per primer. The polymorphism information content (PIC) value for the SSR loci ranged from 0.089 to 0.850 with an average of 0.543. There existed much difference in specific indices of varieties, ranging from 109.1 to 454.7 with an average of 189.0. The results showed that 11 primers (Xtxp329, Xtxp258, Xtxp113, Xtxp303, Xtxp61, Xtxp201, Xtxp141, Xtxp91, Xtxp47, Xtxp217, and Xtxp67) were confirmed on the basis of allele number of each pair of primers. The IDs of 142 sweet sorghum germplasm resources were established by the 11 pairs of primers, which can discriminate a variety from others.

Key words: Sweet sorghum, Germplasm resources, Molecular identity

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