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作物学报 ›› 2012, Vol. 38 ›› Issue (08): 1378-1386.doi: 10.3724/SP.J.1006.2012.01378

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

寒地多年生小麦的选育与细胞遗传学分析

赵海滨1,2，张延明1,2，史春龙1，闫小丹1，田超1，厉永鹏1，李集临1,*   

  1. 1哈尔滨师范大学生命科学与技术学院 / 黑龙江省分子细胞遗传与遗传育种重点实验室, 黑龙江哈尔滨 150025; 2黑龙江省农业科学院作物育种研究所, 黑龙江哈尔滨 150086
  • 收稿日期:2011-09-02 修回日期:2012-04-16 出版日期:2012-08-12 网络出版日期:2012-06-04
  • 通讯作者: 李集临, E-mail: jilinlee2004@yahoo.com.cn, Tel: 0451-88060576
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2011AA10010205), 黑龙江省博士后基金(LBH-Z10034), 黑龙江省高校科技创新团队研究计划, 哈尔滨师范大学科技创新团队研究计划(KJTD-2011-2)和哈尔滨师范大学青年学术骨干资助计划项目(KGB200918)资助。

Development and Cytogenetic Analysis of Perennial Wheat in Cold Region

ZHAO Hai-Bin1,2,ZHANG Yan-Ming1,2,SHI Chun-Long1,YAN Xiao-Dan1,TIAN Chao1,LI Yong-Peng1,LI Ji-Lin1,*   

  1. 1 Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province / College of Life Science and Technology, Harbin Normal University, Harbin 150025, China; 2 Institute of Crop Breeding, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China?
  • Received:2011-09-02 Revised:2012-04-16 Published:2012-08-12 Published online:2012-06-04
  • Contact: 李集临, E-mail: jilinlee2004@yahoo.com.cn, Tel: 0451-88060576

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1374

被引次数

11

摘要： 通过杂交方法获得八倍体小偃麦与中间偃麦草杂种后代，对该杂交后代进行了形态学观察和细胞遗传学分析。杂交当代结实率为10%~39%；F1表现为两亲中间型，多年生，抗小麦多种病害，生长的第2和第3年结少量种子，结实率为2%~3%；F2分离复杂，出现八倍体小偃麦类型和中间偃麦草类型的多年生材料；F3和F4代出现一些普通小麦类型的多年生小麦,表现多分蘖、多小穗、抗病、抗寒。F1根尖减数分裂中发现49条染色体，在减数分裂中期I形成14~17个二价体和4~21个单价体；而F2和F3代减数分裂时形成14~21个二价体和9~17个单价体。杂种后代结实率逐代恢复。F1植株已在田间自然条件下生长5年。从F4代中获得了4个植株高大(140 cm)、分蘖丰富(60个以上)、小穗多(25~30个)的饲草型多年生小麦株系，它们不仅具有良好的刈割再生能力，而且兼抗多种病害，抗寒性好，草质与中间偃麦草相似。还获得了一些普通小麦类型的多年生株系，有待进一步改良。这些结果为多年生小麦的遗传研究和利用提供了信息和材料基础。

关键词: 中间偃麦草, 多年生小麦, 形态特征, 细胞遗传学

Abstract: The morphology of the progeniesfrom octoploid Trititrigia ´ Thinopyrum intermedim and their chromosomal behaviours during meiosis were observed to provide therotical and material bases for developing perennial wheat. The seed-setting rate of contemporary hybrid was 10–39%, and the F1 plants showed an intermediate type of parents with perennial characteristic and resistance to multiple diseases. During the second and third growing year, the F1 plants produced a small amount of seeds with setting rate of 2–3%. The F2 plants separated complexly, which involved the types of Tritielytrigia and Thinopyrum intermedium. The F3 and F4 generations tended to produce some types of common wheat but with perennial characteristics, such as rich tillers, prolific spikelets, and resistances to diseases and coldness. In F1 generation, 49 chromosomes were observed in root tip cells, which formed 14–17 bivalents and 4–21 univalents at metaphase of meiosis I. In F2 and F3 generations, the numbers of bivalent and univalent at meiosis were 14–21 and 9–17, respectively. Seed-setting rate of the perennial hybrids was recovered with the increase of generation, and the F1 plants could survive in natural environment for five years. We have obtained four perennial wheat lines in forage type from the F4 generation. These lines showed tall plant height (140 cm), abundant tillers (more than 60), rich spikelets (25–30), resistance to multiple diseases, tolerance to coldness, and high regenerative capability after harvest. Besides, some common wheat-type perennial lines were also attractive, which requires further improvement.

Key words: Thinopyrum intermedium, Perennial wheat, Morphological characteristics, Cytogenetics

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