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作物学报 ›› 2013, Vol. 39 ›› Issue (03): 398-408.doi: 10.3724/SP.J.1006.2013.00398

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

中国谷子育成品种维生素E含量分布规律及其与主要农艺性状和类胡萝卜素的相关性分析

刘敏轩,陆平*   

  1. 中国农业科学院作物科学研究所,北京 100081
  • 收稿日期:2012-07-04 修回日期:2012-11-16 出版日期:2013-03-12 网络出版日期:2013-01-04
  • 通讯作者: 陆平, E-mail: zaliang@sina.com, Tel: 010-62186625
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-07-12[1]5-A1)和农业部作物种质资源保护项目(NB2012-2130135-25-06-1)资助。

Distribution of Vitamin E Content and Its Correlation with Agronomic Traits and Carotenoids Content in Foxtail Millet Varieties in China

LIU Min-Xuan,LU Ping*   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2012-07-04 Revised:2012-11-16 Published:2013-03-12 Published online:2013-01-04
  • Contact: 陆平, E-mail: zaliang@sina.com, Tel: 010-62186625

摘要:

采用正相高效液相色谱法测定200份不同省份来源的谷子育成品种籽粒中的4种维生素E(α-VEβ-VEγ-VEδ-VE)及叶黄素和玉米黄素2种类胡萝卜素组分的含量,并分析了谷子维生素E含量与类胡萝卜素含量和主要农艺性状的相关性。结果显示,我国谷子育成品种籽粒中VE总量、α-VE含量及(β+γ)-VE含量均呈正态分布,δ-VE含量呈偏态分布,且(β+γ)-VE是维生素E的主要组分; 陕西和吉林谷子的VE总量和(β+γ)-VE含量显著高于其他省份品种,东北三省谷子的α-VE含量显著高于其他省份,而不同省份谷子品种中δ-VE含量差异不显著;不同省份谷子品种间叶黄素含量差异达极显著(P<0.01),玉米黄素含量差异不显著,类胡萝卜素总含量差异显著(P<0.05)。相关分析表明,参试品种籽粒中叶黄素含量与VE总量呈显著正相关,与α-VE含量呈极显著正相关,与δ-VE含量呈极显著负相关,与(β+γ)-VE含量不相关;玉米黄素与VE总量和α-VE含量呈显著正相关,与δ-VE含量呈极显著负相关,与(β+γ)-VE含量不著相关;VE总量和(β+γ)-VE含量与千粒重呈显著负相关,δ-VE含量与粒重和穗重呈极显著正相关,类胡萝卜素各组分与草重呈极显著正相关。

关键词: 谷子育成品种, 维生素E, 类胡萝卜素, 高效液相色谱, 相关分析

Abstract:

Wedetermined vitamin E (VE) and carotenoid contents in 200 foxtail millet (Setaria italica Beauv.) developed varieties from different provinces with high performance liquid chromatography (HPLC) to explore distribution and screen varieties with high VE and carotenoid contents, as well as analyze correlations among total VE content, α-VE content, (β+γ)-VE content, δ-VE content, carotenoid content and agronomic traits. The results showed that the distribution of total VE content, α-VE content and (β+γ)-VE content in foxtail millet varieties were normal, while that of δ-VE content was skwe . (β+γ)-VE content was the most abundant fraction in china foxtail millet varieties. The foxtail millet varieties from Shaanxi and Jilin had the highest total VE contents with significant difference from those of other provinces; those from Heilongjiang, Jilin and Liaoning had highest (β+γ)-VE contents also with significant difference from those of other provinces. There was no significant difference in δ-VE content among varieties of different provinces. Lutein and zeaxanthin were two main carotenoids in foxtail millet varieties. The contents of these carotenoids were extremely different among foxtail millet varieties which came from different provinces. Correlation analysis showed that lutein and zeaxanthin contents were significantly and positively correlated with the total VE content and α-VE content, while significantly and negatively correlated with the δ-VE content. There was no significant correlation between lutein content and (β+γ)-VE content. Total VE content and (β+γ)-VE content were all negatively correlated with 1000-seed weight at 0.05 probability level, δ-VE content was positively correlated with weed weight per plant and spikelet weight at 0.01 probability level. Carotenoid contents were all positively correlated with grass weight at 0.01 probability level.

Key words: Foxtail millet varieties, Vitamin E, Carotenoids, HPLC, Correlation analysis

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