作物学报 ›› 2013, Vol. 39 ›› Issue (04): 589-598.doi: 10.3724/SP.J.1006.2013.00589
刘亚男1,夏先春1,何中虎1,2,*
LIU Ya-Nan1,XIA Xian-Chun1,HE Zhong-Hu1,2,*
摘要:
OsDep1 (dense and erect panicle 1)控制水稻产量性状,影响穗长、直立性和着粒密度。根据水稻OsDep1基因序列,采用同源克隆技术克隆了普通小麦第5同源群染色体上的TaDep1基因,它包含5个外显子和4个内含子,与水稻OsDep1基因结构相似。TaDep1-A1、TaDep1-B1和TaDep1-D1的编码序列长度分别为918、888和900 bp,编码305、295和299个氨基酸残基。在普通小麦品种中检测到5个TaDep1-A1等位变异、4个TaDep1-B1等位变异和2个TaDep1-D1等位变异。根据TaDep1-A1和TaDep1-B1位点不同等位变异间的SNP和InDel,开发了3对显性互补标记和1个共显性标记,可以准确鉴别不同等位基因。共显性标记dep19是根据TaDep1-B1第5外显子一个30 bp的InDel开发的,可准确区分TaDep1-B1c与TaDep1-B1a、TaDep1-B1b和TaDep1-B1d。用这些标记对406份小麦品种进行检测,不同基因型的千粒重、株高、穗长、小穗数和穗节间均差异不显著,说明TaDep1基因与我国现有小麦品种的产量性状相关不显著。
[1]He Z-H(何中虎), Xia X-C(夏先春), Chen X-M(陈新民), Zhuang Q-S(庄巧生). Progress of wheat breeding in China and the future perspective. Acta Agron Sin (作物学报), 2011, 37(2): 202–215 (in Chinese with English abstract)[2]Liu Y N, He Z H, Appels R, Xia X C. Functional markers in wheat: current status and future prospects. Theor Appl Genet, 2012, 125: 1–10[3]Wang R-X(王瑞霞). QTL Analysis of Grain Filling Rate and Related Traits in Wheat (Triticum aestivum L.). PhD Dissertation of Chinese Academy of Agricultural Sciences, 2008 (in Chinese with English abstract)[4]Marza F, Bai G H, Carver B F, Zhou W C. Quantitative trait loci for yield and related traits in the wheat population Ning 7840 × Clark. Theor Appl Genet, 2006, 112: 688–698[5]Li S S, Jia J Z, Wei X Y, Zhang X C, Li L Z, Chen H M, Fan Y D, Sun H Y, Zhao X H, Lei T D, Xu Y F, Jiang F S, Wang H G, Li L H. An intervarietal genetic map and QTL analysis for yield traits in wheat. Mol Breed, 2007, 20: 167–178[6]Huang X Q, Cloutier S, Lycar L, Radovanovic N, Humphreys D G, Noll J S, Somers D J, Brown P D. Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theor Appl Genet, 2006, 113: 753–766[7]Xiao Y-G(肖永贵). Genetic Improvement of Yield Traits in Shandong Wheat Cultivars and Molecular Dissection of Core Parent Zhou 8425B. PhD. Dissertation of Northwest A&F University, 2011 (in Chinese with English abstract)[8]Zhuang Q-S(庄巧生). Wheat Improvement and Pedigree Analysis in Chinese Wheat Cultivars (中国小麦品种改良及系谱分析). Beijing: China Agriculture Press, 2003. p 502 (in Chinese)[9]Quarrie S A, Steed A, Calestani C, Semikhodskii A, Lebreton C, Chinoy C, Steele N, Pljevljakusi? D, Waterman E, Weyen J, Schondelmaier J, Habash D Z, Farmer P, Saker L, Clarkson D T, Abugalieva A, Yessimbekova M, Turuspekov Y, Abugalieva S, Tuberosa R, Sanguineti M-C, Hollington P A, Aragués R, Royo A, Dodig D. A high-density genetic map of hexaploid wheat (Triticum aestivum L.) from the cross Chinese Spring × SQ1 and its use to compare QTLs for grain yield across a range of environments. Theor Appl Genet, 2005, 110: 865–880[10]Kumar N, Kulwal P L, Gaur A, Tyagi A K, Khurana J P, Khurana P, Balyan H S, Gupta P K. QTL analysis for grain weight in common wheat. Euphytica, 2006, 151: 135–144[11]Sun X Y, Wu K, Zhao Y, Kong F M, Han G Z, Jiang H M, Huang X J, Li R J, Wang H G, Li S S. QTL analysis of kernel shape and weight using recombinant inbred lines in wheat. Euphytica, 2009, 165: 615–624[12]Ramya P, Chaubal A, Kulkarni K, Gupta L, Kadoo N, Dhaliwal H S, Chhuneja P, Lagu M, Gupta V. QTL mapping of 1000-kernel weight, kernel length, and kernel width in bread wheat (Triticum aestivum L.). J Appl Genet, 2010, 51: 421–429[13]Jiang Q Y, Hou J, Hao C Y, Wang L F, Ge H M, Dong Y S, Zhang X Y. The wheat (T. aestivum) sucrose synthase 2 gene (TaSus2) active in endosperm development is associated with yield traits. Funct Integr Genomics, 2011, 11: 49–61[14]Su Z Q, Hao C Y, Wang L F, Dong Y C, Zhang X Y. Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2011, 122: 211–223[15]Ma D Y, Yan J, He Z H, Wu L, Xia X C. Characterization of a cell wall invertase gene TaCwi-A1 on common wheat chromosome 2A and development of functional markers. Mol Breed, 2012, 29: 43–52 [16]Kong F N, Wang J Y, Zou J C, Shi L X, Jin D M, Xu Z J, Wang B. Molecular tagging and mapping of the erect panicle gene in rice. Mol Breed, 2007, 19: 297–304[17]Yan C J, Zhou J H, Yan S, Chen F, Yeboah M, Tang S Z, Liang G H, Gu M H. Identification and characterization of a major QTL responsible for erect panicle trait in japonica rice (Oryza sativa L.). Theor Appl Genet, 2007, 115: 1093–1100[18]Zhou Y, Zhu J Y, Li Z Y, Yi C D, Liu J, Zhang H G, Tang S Z, Gu M H, Liang G H. Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication. Genetics, 2009, 183: 315–324[19]Wang J Y, Nakazaki T, Chen S Q, Chen W F, Saito H, Tsukiyama T, Okumoto Y, Xu Z J, Tanisaka T. Identification and characterization of the erect-pose panicle gene EP conferring high grain yield in rice (Oryza sativa L.). Theor Appl Genet, 2009, 119: 85–91[20]Huang X Z, Qian Q, Liu Z B, Sun H Y, He S Y, Luo D, Xia G G, Chu C C, Li J Y, Fu X D. Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet, 2009, 41: 494–497[21]Fan C C, Xing Y Z, Mao H L, Lu T T, Han B, Xu C G, Li X H, Zhang Q F. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor Appl Genet, 2006, 112: 1164–1171[22]Yi X H, Zhang Z J, Zeng S Y, Tian C Y, Peng J C, Li M, Lu Y, Meng Q C, Gu M H, Yan C J. Introgression of qPE9-1 allele, conferring the panicle erectness, leads to the decrease of grain yield per plant in japonica rice (Oryza sativa L.). J Genet Genomics, 2011, 38: 217–223[23]Yan L L, Loukoianov A, Blechl A, Tranquilli G, Ramakrishna W, SanMiguel P, Bennetzen J L, Echenique V, Dubcovsky J. The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science, 2004, 303: 1640–1643[24]He X Y, He Z H, Zhang L P, Sun D J, Morris C F, Fuerst E P, Xia X C. Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theor Appl Genet, 2007, 115: 47–58[25]He X Y, Zhang Y L, He Z H, Wu Y P, Xiao Y G, Ma C X, Xia X C. Characterization of phytoene synthase 1 gene (Psy1) located on common wheat chromosome 7A and development of a functional marker. Theor Appl Genet, 2008, 116: 213–221[26]He X Y, He Z H, Ma W, Appels R, Xia X C. Allelic variants of phytoene synthase 1 (Psy1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for flour colour. Mol Breed, 2009, 23: 553–563[27]Gautier M F, Cosson P, Guirao A, Alary R, Joudrier P. Puroindoline genes are highly conserved in diploid ancestor wheats and related species but absent in tetraploid Triticum species. Plant Sci, 2000, 153: 81–91[28]Lillemo M, Simeone M C, Morris C F. Analysis of puroindoline a and b sequences from Triticum aestivum cv. ‘Penawawa’ and related diploid taxa. Euphytica, 2002, 126: 321–331[29]Chantret N, Salse J, Sabot F, Rahman S, Bellec A, Laubin B, Dubois I, Dossat C, Sourdille P, Joudrier P, Gautier M F, Cattolico L, Beckert M, Aubourg S, Weissenbach J, Caboche M, Bernard M, Leroy P, Chalhoub B. Molecular basis of evolutionary events that shaped the hardness locus in diploid and polyploid wheat species (Triticum and Aegilops). Plant Cell, 2005, 17: 1033–1045 |
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