欢迎访问作物学报,今天是

作物学报 ›› 2007, Vol. 33 ›› Issue (02): 236-241.

• 研究论文 • 上一篇    下一篇

枯草芽孢杆菌B931防治植物病害和促进植物生长的作用

张霞1,2;唐文华1,*;张力群1   

  1. 1中国农业大学植物病理系,北京100094;2上海交通大学生命科学技术学院,上海200240
  • 收稿日期:2006-01-13 修回日期:1900-01-01 出版日期:2007-02-12 网络出版日期:2007-02-12
  • 通讯作者: 唐文华

Biological Control of Plant Diseases and Plant Growth Promotion by Bacillus subtilis B931

ZHANG Xia12,TANG Wen-Hua1*,ZHANG Li-Qun1   

  1. 1 Department of Plant Pathology, China Agricultural University, Beijing 100094; 2 Department of Life Science and Technique, Shanghai JiaoTong University, Shanghai 200240, China
  • Received:2006-01-13 Revised:1900-01-01 Published:2007-02-12 Published online:2007-02-12
  • Contact: TANG Wen-Hua

摘要:

枯草芽孢杆菌(Bacillus subtilis)B931是分离自小麦田的植物病害生物防治细菌,对多种作物土传病害都有很好的防治效果,并能促进作物生长。本研究通过转座子Tn917的转座诱变,构建了菌株B931的突变体库。自3 000多个突变体中,筛选得到了6个对小麦全蚀病菌抑制能力丧失的突变体(B931-A),和多个产生长素、赤霉素、细胞分裂素能力增加的突变体B931-I+、B931-G+、B931-C+,以及产这3类激素能力减少的突变体B931-I、B931-G、B931-C。温室测定这些突变体对小麦全蚀病及棉花立枯病的防治作用,结果表明抗生素的产生是B931具有病害防治能力的主要原因。产植物激素能力变化的突变体对苗期小麦和棉花没有促生长作用,但对甘薯苗的生根有显著的促进作用。

关键词: 枯草芽孢杆菌, 转座诱变, 抗生素, 植物激素

Abstract:

Bacillus subtilis B931, a biological control bacterium isolated from wheat field, protects plants against many soil-borne diseases and promotes plant growth. A mutant library of B931 containing over 3 000 Tn917 mutants was constructed by transposon mutagenesis with plasmid pTV1::Tn917. Six mutants defective in antibiotic production (B931-A-) were selected by dual culture test with Gaeumannomyces graminis var. tritici on PDA plates. Another six mutants which over-produced or less-produced phytohormones 3-Indoleacetic acid (IAA), gibberellin (GA3) or cytokinin (CTK), respectively, were selected by ELISA and HPLC methods. Greenhouse experiments showed that, compared with the wild type, the antibiotic-defective mutant was less effective in biological control of wheat take-all caused by G. graminis var. tritici and cotton damping-off caused by Rhizoctonia solani, indicating that the antibiotic production played a major role in the biocontrol activity of B931. The mutants that over-produced or less-produced phytohormones had no significant influence on seedling growth of wheat and cotton. However, the mutants that overproduced IAA or CTK could significantly promote root growth of sweet potato seedlings.

Key words: Bacillus subtilis, Transposon mutagenesis, Antibiotic, Phytohormone

[1] 蔡兆琴, 何观咏, 何文, 阮丽霞, 梁振华, 李永珍, 李恒锐, 陈会鲜. 木薯分枝发育过程的动态转录组分析与关键基因发掘[J]. 作物学报, 2026, 52(5): 1430-1441.
[2] 马亮, 马璐, 张舒钰, 章慧敏, 王仁明, 宋旭东, 张振良, 冒宇翔, 陆虎华, 陈国清, 郝德荣, 周广飞. 玉米苞叶数目转录组分析及候选基因鉴定[J]. 作物学报, 2026, 52(3): 790-801.
[3] 沈傲, 刘敏, 倪迪安, 刘炜. 谷子m6A甲基转移酶基因SiMTA1的启动子序列特征和基因表达模式分析[J]. 作物学报, 2025, 51(7): 1969-1978.
[4] 宋松泉, 唐翠芳, 程红焱, 王程亮, 袁良兵, 左胜. 谷类作物的胚乳发育及其对种子休眠与萌发的作用[J]. 作物学报, 2025, 51(5): 1133-1155.
[5] 谢章书, 谢学方, 屠小菊, 刘爱玉, 董合忠, 周仲华. 植物激素对棉花蕾铃脱落的调控研究进展[J]. 作物学报, 2025, 51(1): 1-29.
[6] 张慧, 张欣雨, 袁旭, 陈伟达, 杨婷. 烟草叶片响应镉胁迫的差异表达基因鉴定及分析[J]. 作物学报, 2024, 50(4): 944-956.
[7] 张馨月, 秦阳, 李瑞, 黄全生, 王逸茹, 郑军. 玉米穗发芽突变体vp2的基因克隆及功能研究[J]. 作物学报, 2024, 50(11): 2712-2719.
[8] 宋松泉, 唐翠芳, 雷华平, 姜孝成, 王伟青, 程红焱. 种子休眠与萌发调控的研究进展[J]. 作物学报, 2024, 50(1): 1-15.
[9] 王菲菲, 张胜忠, 胡晓辉, 崔凤高, 钟文, 赵立波, 张天雨, 郭进涛, 于豪谅, 苗华荣, 陈静. 比较转录组分析花生种子休眠调控网络[J]. 作物学报, 2023, 49(9): 2446-2461.
[10] 朱继杰, 王士杰, 赵红霞, 贾晓昀, 李妙, 王国印. 田间条件下不同棉花品种叶片响应化学脱叶剂噻苯隆的转录组分析[J]. 作物学报, 2023, 49(10): 2705-2716.
[11] 李长宁,谢金兰,王维赞,梁强,李毅杰,董文斌,刘晓燕,杨丽涛,李杨瑞. 水分胁迫下甘蔗差异表达基因筛选及激素相关基因分析[J]. 作物学报, 2015, 41(07): 1127-1135.
[12] 徐恒恒,黎妮,刘树君,王伟青,王伟平,张红,程红焱,宋松泉. 种子萌发及其调控的研究进展[J]. 作物学报, 2014, 40(07): 1141-1156.
[13] 蔡铁,徐海成,尹燕枰,杨卫兵,彭佃亮,倪英丽,徐彩龙,杨东清,王振林. 外源IAA、GA3和ABA影响不同穗型小麦分蘖发生的机制[J]. 作物学报, 2013, 39(10): 1835-1842.
[14] 李先恩,孙鹏,祁建军,周丽莉,王绍华. 地黄栽培种与野生种内源激素含量的差异[J]. 作物学报, 2013, 39(07): 1276-1283.
[15] 解海岩;蒋培东;王晓玲;张昭伟;朱伟;王学德. 棉花细胞质雄性不育花药败育过程中内源激素的变化[J]. 作物学报, 2006, 32(07): 1094-1096.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!