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作物学报 ›› 2014, Vol. 40 ›› Issue (10): 1846-1856.doi: 10.3724/SP.J.1006.2014.01846

• 耕作栽培·生理生化 • 上一篇    下一篇

荞麦茎秆解剖结构和木质素代谢及其与抗倒性的关系

汪灿1,阮仁武1,2,袁晓辉1,2,胡丹1,杨浩1,林婷婷1,何沛龙1,李燕1,易泽林1,2   

  1. 1 西南大学农学与生物科技学院, 重庆 400716; 2 重庆市荞麦产业体系创新团队, 重庆 400716
  • 收稿日期:2013-12-26 修回日期:2014-07-06 出版日期:2014-10-12 网络出版日期:2014-07-23
  • 通讯作者: 易泽林, E-mail: yzlin1969@126.com, Tel: 023-68251264
  • 基金资助:

    本研究由中央高校基本科研业务费专项资金(XDJK2014D012), 中央高校基本科研业务费专项资金(2362014xk09), 重庆市荞麦产业体系创新团队建设项目(CQCYT2011001)和重庆市科技计划应用开发重点项目(cstc2013yykfb0118)资助。

Relationship of Anatomical Structure and Lignin Metabolism with Lodging Resistance of Culm in Buckwheat

WANG Can1,RUAN Ren-Wu1,2,YUAN Xiao-Hui1,2,HU Dan1,YANG Hao1,LIN Ting-Ting1,HE Pei-Long1,LI Yan1,YI Ze-Lin1,2,*   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China; 2 Chongqing Buckwheat Industry System Innovation Team, Chongqing 400716, China
  • Received:2013-12-26 Revised:2014-07-06 Published:2014-10-12 Published online:2014-07-23
  • Contact: 易泽林, E-mail: yzlin1969@126.com, Tel: 023-68251264

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摘要：

以抗倒伏能力不同的4个荞麦品种研究表明，荞麦茎秆抗倒伏能力与茎秆解剖结构和木质素代谢密切相关。倒伏率与茎秆抗折力参数(r = –0.907, P < 0.01)、木质素含量(r = –0.844, P < 0.01)、机械组织厚度(r = –0.881, P < 0.01)、茎壁厚度(r = –0.947, P < 0.01)、维管束面积(r = –0.846, P < 0.01)、机械组织层数(r = –0.806, P < 0.05)和大维管束数目(r = –0.709, P < 0.05)呈显著负相关，而与倒伏指数(r = 0.842, P < 0.01)呈显著正相关。木质素含量与苯丙氨酸解氨酶(PAL)活性(r = 0.984, P < 0.01)、4-香豆酸:CoA连接酶(4CL)活性(r = 0.927, P < 0.01)和肉桂醇脱氢酶(CAD)活性(r = 0.862, P < 0.01)呈显著正相关。茎秆木质素含量、机械组织层数、机械组织厚度、茎壁厚度、大维管束数目和维管束面积可以作为荞麦茎秆抗倒伏能力的重要评价指标。茎秆木质素含量高、机械组织层数多、机械组织和茎壁厚、大维管束数目多且维管束面积大的荞麦品种，其茎秆抗折力参数大、倒伏指数小、抗倒伏能力强。

关键词: 荞麦, 解剖结构, 质素代谢, 抗倒伏能力

Abstract:

Lodging is a major problem in buckwheat (Fagopyrum esculentum Moench) production. In this study, we investigated the relationship between anatomical structure together with lignin metabolism and lodging resistance. The results indicated that the lodging resistance of culm in buckwheat was closely related to the anatomical structure and lignin metabolism of culm. The lodging percentage was negatively correlated with snapping resistance parameter of culm (r = –0.907, P < 0.01), lignin content (r = –0.844, P < 0.01), mechanical tissue thickness (r = –0.881, P < 0.01), culm wall thickness (r = –0.947, P < 0.01), vascular bundle area (r = –0.846, P < 0.01), mechanical tissue layer number (r = –0.806, P < 0.05), and large vascular bundle number (r = –0.709, P < 0.05), but positively correlated with lodging index (r = 0.842, P < 0.01). The lignin content was positively correlated with activities of phenylalanine ammonialyase (r = 0.984, P < 0.01), 4-coumarate: CoA ligase (r = 0.927, P < 0.01), and cinnamyl alcohol dehydrogenase (r = 0.862, P < 0.01). Therefore, lignin content in culm, mechanical tissue layer number, mechanical tissue thickness, culm wall thickness, large vascular bundle number, and vascular bundle area can be used as main indicators to evaluate lodging resistance in buckwheat. Buckwheat cultivars with high resistance to culm snapping and lodging usually have high lignin content, large number of mechanical tissue layer, and large number of vascular bundle, and thick mechanical tissue and culm wall, and large vascular bundle area.

Key words: Buckwheat, Anatomical structure, Lignin metabolism, Lodging resistance.

[1]Lin R F, Chai Y. Production, research and academic exchanges of China on buckwheat. In: Chai Y, Zhang Z W, eds. Advances in buckwheat research. Yangling: Northwest A&F University Press, 2007. pp 7–12



[2]王红育, 李颖. 荞麦的研究现状及应用前景. 食品科学, 2004, 25(10): 388–391



Wang H Y, Li Y. Research status and applied prospect of buckwheat. Food Sci, 2004, 25(10): 388–391 (in Chinese with English abstract)



[3]Koyama M, Nakamura C, Nakamura K. Changes in phenols contents from buckwheat sprouts during growth stage. J Food Sci Technol, 2013, 50: 86–93



[4]Hagiwara M, Izusawa H, Inoue N, Matano T. Varietal differences of shoot growth characters related to lodging in Tartary buckwheat. Fagopyrum, 1999, 16: 67–72



[5]Jones L, Ennos A R, Turner S R. Cloning and characterization of irregular xylem4 (irx4): a severely lignin-deficient mutant of Arabidopsis. Plant J, 2001, 26: 205–216



[6]Baucher M, Monties B, Van Montagu M, Boerjan W. Biosynthesis and genetic engineer in lignin. Crit Rev Plant Sci, 1998, 17: 125–197



[7]Lewis N G, Yamamoto E. Lignin: occurrence, biogenesis and biodegradation. Annu Rev Plant Physiol Plant Mol Biol, 1990, 41:455–496



[8]Welton F A. Lodging in oats and wheat. Bot Gaz, 1928, 85: 121–151



[9]王群瑛, 胡昌浩. 玉米茎秆抗倒特性的解剖研究. 作物学报, 1991, 17: 70–75



Wang Q Y, Hu C H. Studies on the anatomical structures of the stalks of maize with different resistance to lodging. Acta Agron Sin, 1991, 17: 70–75 (in Chinese with English abstract)



[10]罗茂春, 田翠婷, 李晓娟, 林金星. 水稻茎秆形态结构特性和化学成分与抗倒伏关系综述. 西北植物学报, 2007, 27: 2346–2353



Luo M C, Tian C T, Li X J, Lin J-X. Relationship between morpho-anatomical traits together with chemical components and lodging resistance of stem in rice (Oryza sativa L.). Acta Bot Boreal-Occident Sin, 2007, 27: 2346–2353 (in Chinese with English abstract)



[11]王芬娥, 黄高宝, 郭维俊, 张锋伟, 赵多佳. 小麦茎秆力学性能与微观结构研究. 农业机械学报, 2009, 40(5): 92–95



Wang F E, Huang G B, Guo W J, Zhang F W, Wu J M, Zhao D J. Mechanical properties and micro-structure of wheat stems. Trans Chin Soc Agric Machinery, 2009, 40(5): 92–95 (in Chinese with English abstract)



[12]杨艳华, 朱镇, 张亚东, 赵庆勇, 周丽慧, 王才林. 水稻茎秆解剖结构与抗倒伏能力关系的研究. 广西植物, 2012, 32: 834–839



Yang Y H, Zhu Z, Zhang Y D, Zhao Q Y, Zhou L H, Wang C L. Relationship between anatomic structure of the stem and lodging resistance of rice. Guihaia, 2012, 32: 834–839 (in Chinese with English abstract)



[13]崔海岩, 靳立斌, 李波, 张吉旺, 赵斌, 董树亭, 刘鹏. 遮阴对夏玉米茎秆形态结构和倒伏的影响. 中国农业科学, 2012, 45: 3497–3505



Cui H Y, Jin L B, Li B, Zhang J W, Zhao B, Dong S T, Liu P. Effects of shading on stalks morphology, structure and lodging of summer maize in field. Sci Agric Sin, 2012, 45: 3497–3505 (in Chinese with English abstract)



[14] Peng D L, Chen X G, Yin Y P, Lu K L, Yang W B, Tang Y H, Wang Z L. Lodging resistance of winter wheat (Triticum estivum L.): lignin accumulation and its related enzymes activities due to the application of paclobutrazol or gibberellin acid. Field Crops Res, 2014, 157: 1–7



[15] Kong E Y, Liu D C, Guo X L, Yang W L, Sun J Z, Li X, Zhan K H, Cui D Q, Lin J X, Zhang A M. Anatomical and chemical characteristics associated with lodging resistance in wheat. Crop J, 2013, 1: 43–49



[16] Mi C Q, Zhang X D, Li S M, Yang J Y, Zhu D H, Yang Y. Assessment of environment lodging stress for maize using fuzzy synthetic evaluation. Math Comput Model, 2011, 54: 1053–1060



[17] Abiven S, Heim A, Schmidt M W I. Lignin content and chemical characteristics in maize and wheat vary between plant organs and growth stages: consequences for assessing lignin dynamics in soil. Plant Soil, 2011, 343: 369–378



[18] 刘仲发, 勾玲, 赵明, 张保军. 遮阴对玉米茎秆形态特征、穿刺强度及抗倒伏能力的影响. 华北农学报, 2011, 26(4): 91–96



Liu Z F, Gou L, Zhao M, Zhang B J. Effects of shading on stalk morphological characteristics, rind penetration strength and lodging resistance of maize. Acta Agric Boreal-Sin, 2011, 26(4): 91–96 (in Chinese with English abstract)



[19] 乔春贵. 作物抗倒伏性的综合指标——倒伏指数. 吉林农业大学学报, 1988, 10(1): 7–10



Qiao C G. Lodging index—a synthetic indication of lodging resistance. J Jilin Agric Univ, 1988, 10(1): 7–10 (in Chinese with English abstract)



[20] 陈晓光, 史春余, 尹燕枰, 王振林, 石玉华, 彭佃亮, 倪英丽, 蔡铁. 小麦茎秆木质素代谢及其与抗倒性的关系. 作物学报, 2011, 37: 1616–1622



Chen X G, Shi C Y, Yin Y P, Wang Z L, Shi Y H, Peng D L, Ni Y L, Cai T. Relationship between lignin metabolism and lodging resistance in wheat. Acta Agron Sin, 2011, 37: 1616–1622 (in Chinese with English abstract)



[21] 魏凤珍, 李金才, 王成雨, 屈会娟, 沈学善. 氮肥运筹模式对小麦茎秆抗倒性能的影响. 作物学报, 2008, 34: 1080–1085



Wei F Z, Li J C, Wang C Y, Qu H J, Shen X S. Effects of nitrogenous fertilizer application model on culm lodging resistance in winter wheat. Acta Agron Sin, 2008, 34: 1080–1085 (in Chinese with English abstract)



[22] 林葵, 黄祥辉, 王隆华, 李人圭, 颜季琼. 甜菜子叶不定芽分化过程中PAL活性和木质素含量变化研究. 华东师范大学学报(自然科学版), 1996, (2): 92–97



Lin K, Huang X H, Wang L H, Li R G, Yan J Q. The change of PAL activity and lignin content during adventitious buds formation of cotyledon in Cucumis melon L. J East China Normal Univ (Nat Sci), 1996, (2): 92–97 (in Chinese with English abstract)



[23] 张志良, 瞿伟菁. 植物生理学实验指导(第3版). 北京: 高等教育出版社, 2003. pp 277–278



Zhang Z L, Qu W J. Experimental Guide for Plant Physiology, 3rd edn. Beijing: Higher Education Press, 2003. pp 277–278 (in Chinese)



[24] Kofalvi S A, Nassuth A. Influence of wheat streak mosaic virus infection on phenylpropanoid metabolism and the accumulation of phenolics and lignin in wheat. Physiol Mol Plant Pathol, 1995, 47: 365–377



[25] Knobloch K H, Hahlbrock K. Isoenzymes of p-coumarate: CoA ligase from cell suspension cultures of Glycine max. Eur J Biochem, 1975, 52: 311–320



[26] Morrison T A, Kessler J R, Hatfield R D, Buxton D R. Activity of two lignin biosynthesis enzymes during development of a maize internode. J Sci Food Agric, 1994, 65: 133–139



[27] Turner S R, Somerville C R. Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall. Plant Cell, 1997, 9: 689–701



[28] Berry P M, Spink J, Sterling M, Pickett A A. Methods for rapidly measuring the lodging resistance of wheat cultivars. J Agron Crop Sci, 2003, 189: 390–401



[29] Tripathi S C, Sayre K D, Kaul J N. Growth and morphology of spring wheat (Triticum aestivum L.) culms and their association with lodging: effects of genotypes, N levels and ethephon. Field Crops Res, 2003, 84: 271–290



[30] 黄杰恒, 李威, 曲存民, 刘列钊, 徐新福, 王瑞, 李加纳. 甘蓝型油菜不同抗倒性材料中木质素代谢途径关键基因表达特点. 作物学报, 2013, 39: 1339–1344



Huang J H, Li W, Qu C M, Liu L Z, Xu X F, Wang R, Li J N. Expression characteristics of key genes in lignin pathway among different lodging resistance lines of Brassica napus L. Acta Agron Sin, 2013, 39: 1339-1344 (in Chinese with English abstract)



[31] 罗自生. GA3处理对采后竹笋木质化及内源激素水平的影响. 园艺学报, 2005, 32: 454–457



Luo Z S. Effect of GA3 treatment on lignification and endogenous hormone levels of postharvest bamboo shoots. Acta Hort Sin, 2005, 32: 454–457 (in Chinese with English abstract)



[32] 魏建华, 宋艳茹. 木质素生物合成途径及调控的研究进展. 植物学报, 2001, 43: 771–779



Wei J H, Song Y R. Recent advances in study of lignin biosynthesis and manipulation. Acta Bot Sin, 2001, 43: 771–779 (in Chinese with English abstract)



[33] 耿飒, 徐存拴, 李玉昌. 木质素生物合成及其调控研究进展. 西北植物学报, 2003, 23: 171–181



Geng S, Xu C S, Li Y C. Advance in biosynthesis of lignin and its regulation. Acta Bot Boreal-Occident Sin, 2003, 23: 171–181 (in Chinese with English abstract)



[34] Kajita S, Hishiyama S, Tomimura Y, Katayama Y, Omori S. Structural characterization of modified lignin in transgenic tobacco plants in which the activity of 4-coumarate: coenzyme a ligase is depressed. Plant Physiol, 1997, 114: 871–879



[35] Hu W J, Harding S A, Lung J, Popko J L, Ralph J, Stokke D D, Tsai C J, Chiang V L. Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees. Nat Biotechnol, 1999, 17: 808–812



[36] Boudet A M, Kajita S, Grima-Pettenati J, Goffner D. Lignins and lignocellulosics: a better control of synthesis for new and improved uses. Trends Plant Sci, 2003, 8: 576–581



[37] 管延安, 李建和, 任莲菊, 李根英. 禾谷类作物倒伏性的研究. 山东农业科学, 1998, (5): 51–54



Guan Y A, Li J H, Ren L J, Li G Y. Study on lodging resistance of cereal crops. J Shandong Agric Sci, 1998, (5): 51–54 (in Chinese)



[38] Pinthus M J. Lodging in wheat, barely, and oats: the phenomenon, its cause, and preventive measure. Adv Agron, 1973, 25: 209–263
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