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作物学报 ›› 2017, Vol. 43 ›› Issue (03): 332-342.doi: 10.3724/SP.J.1006.2017.00332

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

Pm52——小麦品种良星99抗白粉病基因的有效性

邹景伟1,2,邱丹1,2,孙艳玲2,郑超星4,李静婷3,吴培培2,武小菲2,王晓鸣2,周阳2,李洪杰2,*   

  1. 1 河北科技师范学院生命科技学院, 河北秦皇岛 066604; 2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京 100081; 3 平顶山学院化学与环境工程学院, 河南平顶山 467000; 4 北京师范大学生命科学学院, 北京 100875
  • 收稿日期:2016-06-11 修回日期:2016-09-18 出版日期:2017-03-12 网络出版日期:2016-09-29
  • 通讯作者: 李洪杰, E-mail: lihongjie@caas.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31471491, 31501310), 国家转基因生物新品种培育科技重大专项(2014ZX0800906B-003)和中国农业科学院农业创新工程项目资助。

Pm52: Effectiveness of the Gene Conferring Resistance to Powdery Mildew in Wheat Cultivar Liangxing 99

ZOU Jing-Wei1,2,QIU Dan1,2,SUN Yan-Ling2,ZHENG Chao-Xing3,LI Jing-Ting4,WU Pei-Pei2,WU Xiao-Fei2,WANG Xiao-Ming2,ZHOU Yang2,LI Hong-Jie2,*   

  1. 1 College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;   3 College of Chemistry and Environment Engineering, Pingdingshan College, Pingdingshan 467000, China; 4 College of Life Science, Beijing Normal University, Beijing 100875, China
  • Received:2016-06-11 Revised:2016-09-18 Published:2017-03-12 Published online:2016-09-29
  • Contact: LI Hongjie, E-mail: lihongjie@caas.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31471491 and 31501310), the National Major Project for Developing New GM Crops (2014ZX0800906B-003), and the Agricultural Science and Technology Innovation Program of CAAS.

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摘要：

良星99是黄淮冬麦区和北部冬麦区推广的抗白粉病冬小麦品种, 其抗白粉病基因位于2BL染色体, 已被命名为Pm52。利用来自不同小麦生产区的123个小麦白粉菌菌株进行抗性鉴定, 良星99可抗80%的菌株。2012-2016年连续5个生长季抗性鉴定中, 良星99在成株期对接种的白粉菌混合菌株都表现免疫或高抗。采用Pm52基因紧密连锁分子标记Xgwm120和27个菌株对10个利用良星99培育的品系进行分子检测和抗性分析发现, 衡4568、邯农2312、中信麦99和DH51302可能携带Pm52基因, 而郑麦369和冀麦729的白粉病抗性基因可能与Pm52不同。石U09-4366、XR4429、衡10-5039和农大3486苗期和成株期都表现感病, 不含Pm52基因。这些品种的成株期抗性反应与苗期的抗性反应一致。本研究的结果有利于良星99的抗白粉病基因Pm52在育种和生产上的有效利用。

关键词: Pm52, Liangxing 99, Wheat powdery mildew, Resistance

Abstract:

Liangxing 99 is a powdery mildew-resistant winter wheat cultivar adapted to the Yellow–Huai Rivers Valley Facultative Wheat Zone and the Northern Winter Wheat Zone. Its powdery mildew resistance is conferred by gene Pm52 on chromosome 2BL. A total of 123 isolates of Blumeria graminis f. sp. tritici collected from different wheat producing regions were used in assessment of disease resistance, and Liangxing 99 was resistant to 80% of them. During the five consecutive growing seasons from 2012 to 2016, Liangxing 99 was immune or highly resistant to the mixture of Bgt isolate inoculated at the adult plant stage. A gene-specific marker Xgwm120 closedly linked to Pm52 and 27 Bgt isolates were used to analyze ten wheat cultivars with Liangxing 99 as a parent. Heng 4568, Hannong 2312, Zhongxinmai 99, and DH51302 may inherit Pm52 from Liangxing 99, while the powdery mildew resistance genes of Zhengmai 369 and Jimai 729 may differ from Pm52. Shi U09-4366, XR4429, Heng 10-5039, and Nongda 3486 were susceptible to all the Bgt isolates examined and did not carry gene Pm52. The responses of these cultivars at the adult plant stage were consistent with those at the seedling stage. Results from this study are favorable to facilitate the effective application of Pm52 from Liangxing 99 in breeding programs and the production of wheat.

Key words: Pm52, Liangxing 99, Wheat powdery mildew, Resistance

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