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作物学报 ›› 2017, Vol. 43 ›› Issue (03): 343-353.doi: 10.3724/SP.J.1006.2017.00343

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

云南哈尼梯田当前栽培水稻ALK基因的遗传多样性及与稻米糊化温度的关联分析

李闯1,2,**,刘承晨1,2,**,张昌泉1,朱霁晖1,徐小颖2,赵福伟3,黄绍文4,金银根2,*,刘巧泉1,*   

  1. 1 扬州大学江苏省作物遗传生理国家重点实验室培育建设点 / 粮食作物现代产业技术协同创新中心,江苏扬州 225009; 2 扬州大学生物科学与技术学院,江苏扬州 225009; 3 环境保护部南京环境科学研究所,江苏南京 210042; 4 云南红河学院,云南蒙自 661100
  • 收稿日期:2016-06-14 修回日期:2016-09-18 出版日期:2017-03-12 网络出版日期:2016-09-29
  • 通讯作者: 刘巧泉, E-mail: qqliu@yzu.edu.cn; 金银根, E-mail: ygenjin@hotmail.com
  • 基金资助:

    本研究由国家重点基础研究发展计划项目(2013CBA01402), 国家自然科学基金项目(31561143008, 31300324)和江苏省高校自然科学研究面上项目(13KJB180028)资助。

Genetic Diversity of ALK Gene and Its Association with Grain Gelatinization Temperature in Currently Cultivated Rice Landraces from Hani’s Terraced Fields in Yunnan Province

LI Chuang1,2,**, LIU Cheng-Chen1,2,**, ZHANG Chang-Quan1, ZHU Ji-Hui1, XU Xiao-Ying2, ZHAO Fu-Wei 3, HUANG Shao-Wen4, JIN Yin-Gen2,*,LIU Qiao-Quan1,*   

  1. 1Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China; 2 College of Biological Science and Technology, Yangzhou University, Yangzhou 225009, China; 3 Nanjing Institute of Environmental Sciences of Ministry of Environmental Protection, Nanjing 210042, China; 4 Yunnan Honghe University, Mengzi 661100, China
  • Received:2016-06-14 Revised:2016-09-18 Published:2017-03-12 Published online:2016-09-29
  • Contact: 刘巧泉, E-mail: qqliu@yzu.edu.cn; 金银根, E-mail: ygenjin@hotmail.com
  • Supported by:

    This study was supported by the National Basic Research Program of China (2013CBA01402), the National Natural Science Foundation of China (31561143008, 31300324)and the Natural Sciences Research Project of Higher Learning Institution in Jiangsu Province (13KJB180028).

摘要:

云南是世界公认的亚洲栽培稻遗传多样性和起源中心之一。利用51对SSR分子标记对云南哈尼梯田当前栽培的111份水稻材料进行多态性检测,并分析其群体结构。聚类分析表明,供试材料主要分为偏粳类群(group I)及偏籼类群(group II)两大类群,其中以偏籼类群居多,占83%,这两类群并未完全按地理来源聚类。所选材料稻米的糊化温度变异较广,可以分为低(<66°C)、中低(66~70°C)、中高(70~74°C)和高(>74°C)等4类。通过基因测序,分析了稻米糊化温度控制基因ALK的序列多样性,发现其可以分为10种单倍型。关联分析结果表明,所选水稻样品稻米的糊化温度主要由ALK基因3个SNP位点组合控制。其中,A-GC和G-TT两种组合主要控制低(<66°C)和中低(66~70°C)糊化温度,G-GC组合主要控制中高(70~74°C)和高(>74°C)糊化温度。SNP3402 T类型与高、中高糊化温度有关。绝大多数偏籼类品种的ALK基因含有G-GC组合,因此都倾向于高糊化温度。上述研究为水稻分子育种和种质资源保护研究提供了一定的参考价值。

关键词: 水稻, ALK基因, 遗传多样性, 哈尼梯田, 糊化温度

Abstract:

Yunnan province is one of the centers for genetic diversity of cultivated rice in Asia. A total of 111 rice landraces were collected from Hani’s rice terraces of Yunnan, and their population structure was analyzed by using 51 SSR molecular markers. The 111 rice landraces were divided into japonica group (group I) and indica group (group II); however, each of the two groups was not completely clustered according to its geographical origin. Most of the collected landraces, accounting for 83%, were indica. Gelatinization temperature (GT) is an important parameter affecting rice cooking and eating quality. The GT of rice flour was measured, which was widely different among the collected rice varieties, showing four classed very low (< 66°C), low (66-70°C), high (70-74°C) and very high ( >74°C). Meanwhile, ALK gene, also known as SSSIIa and controlling GT, was sequenced and compared, showing ten haploid types of the ALK gene among tested samples. Association analysis, revealed that GT is mainly controlled by the combination of three SNPs of ALK gene. Among them, the A-GC or G-TT combination type controls the very low and low GT, while the combination of G-GC controls high and very high GT. SNP3402 type T is associated with high and very high GT. It was also noticed that most of the varieties from indica group tended to contain the G-GC mutation and thus showed a relative high GT compared with those from the japonica group. The results have a meaningful value for rice molecular breeding and germplasm resources protection.

Key words: Oryza sativa L., ALK gene, Genetic diversity, Hani’s terrace, Gelatinization temperature

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