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作物学报 ›› 2017, Vol. 43 ›› Issue (03): 354-370.doi: 10.3724/SP.J.1006.2017.00343

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

陆地棉幼苗NaCl胁迫下转录因子表达的转录组学分析

彭振1,2,何守朴1,龚文芳1,潘兆娥1,贾银华1,卢艳丽2,*,杜雄明1,*   

  1. 1中国农业科学院棉花研究所 /棉花生物学国家重点实验室,河南安阳455000; 2四川农业大学玉米研究所,四川温江 611130
  • 收稿日期:2016-05-12 修回日期:2015-09-18 出版日期:2017-03-12 网络出版日期:2016-09-28
  • 通讯作者: 杜雄明, E-mail: dxm630723@163.com;卢艳丽, E-mail: yanli.lu82@hotmail.com
  • 基金资助:

    本研究由“十二五”国家支撑计划项目(2013BAD01B03)和国家自然科学基金项目(31301365)资助。

Transcriptome Analysis of Transcription Factors Expression PatterninUpland Cotton Seedlings under NaCl Stress

PENG Zhen1,2,HE Shou-Pu1,GONG Wen-Fang1,PAN Zhao-E1,JIA Yin-Hua1,LU Yan-Li2,*,DU Xiong-Ming1,*   

  1. 1 Institute of Cotton Research, Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang 455000, China; 2 Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
  • Received:2016-05-12 Revised:2015-09-18 Published:2017-03-12 Published online:2016-09-28
  • Contact: 杜雄明, E-mail: dxm630723@163.com;卢艳丽, E-mail: yanli.lu82@hotmail.com
  • Supported by:

    This study was supported by the National Science and Technology Support Program of China (2013BAD01B03) and the National Natural Science Found (31301365)。

摘要:

以棉花耐盐种质早熟长绒7号和感盐种质南丹巴地大花为材料,利用转录组测序(RNA-Seq)技术分析2个种质在200 mmol L–1 NaCl胁迫4和24 h后幼苗叶片的转录因子家族及转录因子的表达情况。结果表明,从样本中鉴定出54个转录因子家族的2815个转录因子。盐胁迫4 h后,长绒7号有249个转录因子基因表达发生变化;南丹巴地大花中有261个转录因子基因表达发生变化。在胁迫24 h后, 2个种质对盐响应的转录因子数量均剧增。只在耐盐种质长绒7号特异表达的有106个(4 h)和184个(24 h)转录因子基因,对于2个种质共同表达的转录因子有143个(4 h)和282个(24 h)。通过筛选,鉴定出26个与耐盐相关的转录因子家族124个转录因子基因,并采用荧光定量验证转录组数据的准确性。推测高表达的11个转录因子的基因CotAD_66280 (HD-ZIP)、CotAD_47058 (ERF)、CotAD_18472 (G2-like)、CotAD_04289 (C2H2)、CotAD_57763 (HSF)、CotAD_23656 (TCP)、CotAD_02221 (ERF)、CotAD_23975 (WRKY)、CotAD_54036 (MYB)、CotAD_27788 (NAC)和CotAD_23815 (HSF) 有可能与陆地棉抗盐性密切相关,可作为陆地棉耐盐育种的候选基因源。

关键词: 陆地棉, NaCl胁迫, 转录组分析, 转录因子

Abstract:

A comparative transcriptomics was used to analyze the expression changes of transcription factors and their families in leaves of the two cotton (Gossypium hirsutum L.) varieties (Earlistaple 7 with salt-tolerance and Nandanbadidahua with salt-sensitive) under 200 mmolL–1 NaCl stress for 4 and 24 hours. We have identified and quantified 2815 transcription factors from 54 transcription factor families of the samples. A total of 249 differentially expressed transcription factor genes of ‘Earlistaple 7’ and 261 transcription factor genes of ‘Nandanbadidahua’ after 4 hours of NaCl stress were identified, respectively. With prolonging stress time (24 hours), the number of differentially expressed transcription factors in two cotton varieties responsive to salt was increased. It was found that the 106 (4 hours) and 184 (24 hours) transcription factor genes were specially expressed in salt-tolerant variety, and the 143 (4 hours) and 282 (24 hours) were exhibited commonly in both cotton lines. We have obtained 124 salt-tolerance-related transcription factor genes from 26 transcription factor families through screening, and further validated the data accuracy by quantitative Real-time PCR.Total 11 highly expressed transcription factors genes by salt induction, including CotAD_66280 (HD-ZIP), CotAD_47058 (ERF), CotAD_18472 (G2-like), CotAD_04289 (C2H2), CotAD_57763 (HSF), CotAD_23656 (TCP), CotAD_02221 (ERF), CotAD_23975 (WRKY), CotAD_54036 (MYB), CotAD_27788 (NAC), and CotAD_23815 (HSF) were induced to be closely related to salt tolerance in upland cotton, and might be used as a source of candidate tolerant genes for upland cotton breeding.

Key words: Upland cotton, NaCl Stress, Transcriptome analysis, Transcription factor

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