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作物学报 ›› 2021, Vol. 47 ›› Issue (5): 807-813.doi: 10.3724/SP.J.1006.2021.03039

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

氮响应转录因子ZmNLP5影响玉米根系生长的功能研究

葛敏(), 王元琮, 宁丽华, 胡梦梅, 石习, 赵涵*()   

  1. 江苏省农业科学院种质资源与生物技术研究所/江苏省农业生物学重点实验室, 江苏南京 210014
  • 收稿日期:2020-06-20 接受日期:2020-11-13 出版日期:2021-05-12 网络出版日期:2020-12-15
  • 通讯作者: 赵涵
  • 作者简介:E-mail: gemin8614@163.com
  • 基金资助:
    国家自然科学基金项目(32001564);江苏省重点研发计划项目(BE207365);江苏省农业科技自主创新资金项目(CX(18)1001)

Function analysis of nitrogen-responsive transcription factor ZmNLP5 affecting root growth in maize

GE Min(), WANG Yuan-Cong, NING Li-Hua, HU Meng-Mei, SHI Xi, ZHAO Han*()   

  1. Institute of Crop Germplasm and Biotechnology / Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China
  • Received:2020-06-20 Accepted:2020-11-13 Published:2021-05-12 Published online:2020-12-15
  • Contact: ZHAO Han
  • Supported by:
    National Natural Science Foundation of China(32001564);China Key Research and Development Program of Jiangsu Province(BE207365);Jiangsu Agriculture Science and Technology Innovation(CX(18)1001)

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摘要：

研究作物氮高效机制并挖掘利用其中重要的调控基因, 对我国农业绿色发展具有重要意义。前期我们发现氮响应转录因子ZmNLP5调控亚硝酸还原酶基因的表达, 对玉米氮素吸收利用具有促进作用, 但其调控机制尚未明确。基于此, 本研究以ZmNLP5基因突变体(zmnlp5)和野生型(WT)植株为研究材料, 深入解析ZmNLP5影响玉米氮素吸收利用的生理机制。足氮(sufficient nitrogen, SN)和低氮(deficient nitrogen, DN)条件下研究材料表型分析发现, DN条件下相对于WT植株, zmnlp5植株根长显著降低。根部不同区域ZmNLP5表达量分析发现, ZmNLP5主要在根尖区域表达。不同亚硝酸盐浓度处理下, 研究材料的根长和根尖区域亚硝酸盐含量分析发现, 当亚硝酸盐的浓度高于2 mmol L-1时, zmnlp5突变体根长相对于WT显著降低, zmnlp5植株根尖区域亚硝酸盐积累量显著高于WT。SN和DN条件下研究材料根部亚硝酸盐含量检测发现, DN条件zmnlp5植株根尖区域所积累的亚硝酸盐也显著高于WT。综上所述, 转录因子ZmNLP5在玉米植株响应低氮环境根部伸长生长中发挥重要功能, 该结果为玉米氮高效育种提供了一定的理论基础。

关键词: 玉米, 氮素, 转录因子, 氮响应, 根长

Abstract:

Improving nitrogen (N) use efficiency of crops is crucial for minimizing N loss and reducing environmental pollution, which is a requirement for the sustainable agriculture. Our previous study found that the transcription factor ZmNLP5 directly regulated the expression of ZmNIR1.1 and promoted nitrogen uptake and assimilation in maize, however, its underlying regulation mechanism is unclear. Here, we performed further phenotype analysis of zmnlp5 and wild type (W22) plants in hydroponic culture on sufficient nitrogen (SN) solution and deficient nitrogen (DN) solution. Compared with WT plants, the root length of zmnlp5 mutant plants was significantly decreased under DN condition. Compared with upper and middle regions of roots, ZmNLP5 was predominantly expressed in root tip regions. Then, we examined the relationship between root length and nitrite content in root tips under a series of nitrite concentrations. The results showed that there was no significant differences in root length between WT and zmnlp5 until the nitrite concentration reached 2 mmol L-1 and higher; however, when the concentration of nitrite was higher than 2 mmol L-1, the root length of zmnlp5 was significantly shorter than WT, and the accumulation of nitrite in the root tips of zmnlp5 was significantly higher than WT. Interestingly, zmnlp5 plants also accumulated significantly more nitrite in the root tips than WT under DN condition. The study showed that the transcription factor ZmNLP5 played an important role in the root growth of maize in response to deficient nitrogen condition, and provided the candidate genes for breeding of maize nitrogen use efficiency in the future.

Key words: maize, nitrogen, transcription factor, nitrogen response, root length

图1

足氮和低氮条件下野生型和zmnlp5突变体材料根长分析 A: 表型鉴定(SN: 足氮; DN: 低氮; 标尺为20 mm); B: 根长分析(** P ≤ 0.01, t测验, n = 3)。"

图2

根部不同区域ZmNLP5表达量分析 A: mRNA表达量分析, 内参基因为ZmUPF1, 显著差异用不同字母表示(P ≤ 0.05); B: WB检测根部不同区域中ZmNLP5蛋白的表达量, UDPGP为内参蛋白。"

图3

亚硝酸盐处理下野生型和zmnlp5突变体材料根长分析 A: 表型鉴定(标尺=20 mm); B: 根长分析(* P ≤ 0.05, t测验, n = 3)。"

图4

亚硝酸盐处理下野生型和zmnlp5突变体材料根尖亚硝酸盐含量"

图5

2种氮环境下野生型和zmnlp5突变体材料根部不同区域亚硝酸盐含量"

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