作物学报 ›› 2019, Vol. 45 ›› Issue (5): 798-805.doi: 10.3724/SP.J.1006.2019.84110
• 研究简报 • 上一篇
Cui CUI,Chuang CHENG,Yu-Feng ZHAO,Huan-Huan GAO,Rui-Li WANG,Liu-Yan WANG,Qing-Yuan ZHOU()
摘要:
随着土壤酸化的日益加重, 铝毒成为影响作物种子萌发质量以及产量的重要胁迫因子之一。本研究采用单项耐铝毒系数(AC值)、综合耐铝毒系数(CAC值)、平均隶属函数值(ASF值)、耐性综合评价值(A值)等指标及相关分析、主成分分析、聚类分析和逐步回归分析等方法, 综合评价豌豆种质萌发期铝毒耐性, 建立综合筛选评价体系, 并筛选萌发期耐铝毒豌豆种质。利用筛选出的适宜浓度40 mg L -1 Al 3+处理52份豌豆品种(系)种子, 结果显示, 萌发期8个相关性状在处理组及对照组品种间均存在显著差异, 遗传多样性指数分别介于1.43~2.03和1.51~2.06之间。基于AC值聚类分析, 52份豌豆种质可被分为3个耐性级别, 其中, 第I级含2份, 为耐铝毒品种(系); 第II级含19份, 为敏感品种(系); 第III级含31份, 为铝毒极敏感性品种(系)。利用豌豆种子发芽率、发芽势、发芽指数、芽长、根长、芽干重、根干重7项指标, 结合最优回归方程可初步鉴定豌豆萌发期耐铝毒特性。通过综合评价和聚类分析, 筛选出萌发期铝毒耐性极强的种质C175和C145, 可作为豌豆铝毒耐性育种和耐性机制研究的重要资源。
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