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作物学报 ›› 2019, Vol. 45 ›› Issue (10): 1455-1467.doi: 10.3724/SP.J.1006.2019.91003

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

70份国外小麦品种(系)的苗期和成株期抗叶锈病鉴定

郑慧敏1,温晓蕾1,2,郝晨阳3,张培培1,GEBREWAHID Takele Weldu1,闫晓翠1,刘大群1,张学勇3,*(),李在峰1,*()   

  1. 1河北农业大学植物保护学院 / 河北省农作物病虫害生物防治工程技术研究中心, 河北保定 071001
    2河北科技师范学院, 河北秦皇岛 066000
    3中国农业科学院作物科学研究所, 北京100081
  • 收稿日期:2019-01-07 接受日期:2019-05-12 出版日期:2019-10-12 网络出版日期:2019-09-10
  • 通讯作者: 张学勇,李在峰
  • 基金资助:
    本研究由国家自然科学基金项目(31571662);本研究由国家自然科学基金项目(31601299);引进国际先进农业科学技术项目资助(2016-X16)

Seedling and slow rusting resistance to leaf rust in 70 introduced wheat lines

ZHENG Hui-Min1,WEN Xiao-Lei1,2,HAO Chen-Yang3,ZHANG Pei-Pei1,GEBREWAHID Takele Weldu1,YAN Xiao-Cui1,LIU Da-Qun1,ZHANG Xue-Yong3,*(),LI Zai-Feng1,*()   

  1. 1College of Plant Protection, Hebei Agricultural University / Biological Control Center of Plant Disease and Plant Pests of Hebei Province, Baoding 071000, Hebei, China
    2Hebei Normal University of Science & Technology, Qinhuangdao 066000, Hebei, China
    3Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-01-07 Accepted:2019-05-12 Published:2019-10-12 Published online:2019-09-10
  • Contact: Xue-Yong ZHANG,Zai-Feng LI
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31571662);This study was supported by the National Natural Science Foundation of China(31601299);the Program of Introducing International Super Agricultural Science and Technology (948 Program)(2016-X16)

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摘要：

小麦叶锈病是小麦生产中的重要病害之一, 培育持久抗病品种是最经济、有效和环保的方法。本研究用19个不同毒力的叶锈菌小种苗期接种70份国外引进小麦品种(系)及36个已知抗叶锈病基因的载体品种进行抗性鉴定, 同时在2016—2017年度分别于河北保定和河南周口对70份国外引进品种进行田间抗叶锈性鉴定。为进一步检测材料中所携带的苗期和成株抗叶锈病基因, 利用12个与已知基因紧密连锁的分子标记进行检测, 综合基因推导、系谱分析和分子标记检测的结果, 在33份材料中鉴定出15个抗叶锈病基因, 包括Lr1、Lr2a、Lr26、Lr3ka、Lr11、Lr17、Lr30、Lr10、Lr14a、Lr2b、Lr13、Lr15、Lr21、Lr44和Lr45, 田间鉴定筛选出39份品种表现慢锈性。苗期和田间表现表明, 国外品种中含有丰富的对我国叶锈菌小种有效的苗期和成株期抗叶锈病基因, 可作为小麦抗叶锈病抗源在抗病育种中加以利用。

关键词: 国外引进小麦, 叶锈病, 基因鉴定, 慢锈性

Abstract:

Leaf rust is one of the most important wheat diseases which has a great influence on yield. Breeding cultivars for durable resistance can effectively and economically control the disease. In this study, seventy introduced wheat varieties, including susceptible control Zhengzhou 5389 and thirty-six donor lines, were inoculated with 19 Chinese pathotypes of Puccinia triticina for leaf rust resistance genes postulation at seedling stage, and to detect APR genes at adult plant stage during the 2016-2017 cropping seasons in Zhoukou of Henan province and Baoding of Hebei province. Zhengzhou 5389 and Saar lines as a susceptible and resistance control, respectively, and thirty-six tester lines with known Lr genes were also used in the present study. Based on the results of gene postulation, marker-assisted detection and pedigree analysis, fifteen Lr genes, including Lr1, Lr2a, Lr26, Lr3ka, Lr11, Lr17, Lr30, Lr10, Lr14a, Lr2b, Lr13, Lr15, Lr21, Lr44, and Lr45, were identified among the tested cultivars. Wheat cultivars with the identified resistance genes in the present study can be used for breeding resistant cultivars of wheat leaf rust in China.

Key words: introduced wheat of abroad, leaf rust, identification of genes, slow-rusting resistance

图1

Lr1、Lr9、Lr10、Lr19、Lr20、Lr24、Lr26、Lr34和Lr37部分PCR标记检测电泳图"

图2

Lr46分子标记检测结果"

表1

36个已知抗叶锈病基因对19个叶锈菌生理小种侵染型a"

表2

70份小麦品种（系）对19个小麦叶锈菌生理小种的侵染型"

表3

70个小麦品种及慢锈SAAR和感病对照在2016-2017年分别在保定与周口最终病害严重度的方差分析"

变异来源
Source of variation		 均方
MS		 F值F-value P值
P-value
品种Variety (V) 853.62 14.43** <0.0001
环境Environment (E) 9660.50 163.35** <0.0001
重复Replicate (R) 5724.50 66.80 0.0734
品种×环境V × E 218.71 3.7** <0.0001
品种×重复V × R 53.85 0.91 0.6532
误差Error 59.14

表4

慢锈品种SAAR、感病对照品种5389及具有慢锈性品种苗期对混合小种的反应型及成株期在2016-2017年的最终病害严重度"

编号
No.		 品种
Variety		 苗期对混合
小种抗性
Seedling IT to
mix species		 2017保定
严重度1
FDS of Baoding
(%)		 2017保定
严重度2
FDS of Baoding
(%)		 2017周口
严重度1
FDS of Zhoukou
(%)		 2017周口
严重度2
FDS of Zhoukou
(%)		 均值
Average FDS
(%)
SAAR 3 5.00 10.00 1.00 15.00 7.75
5389 4 90.00 85.00 85.00 90.00 87.50
2 Satanta 4 5.00 5.00 15.00 30.00 13.75
5 TAM 105 4 5.00 1.00 35.00 20.00 15.25
6 Early Red Fife 4 5.00 25.00 15.00 25.00 17.50
10 Redman 4 1.00 30.00 1.00 1.00 8.25
12 Neepawa 4 5.00 20.00 15.00 25.00 17.50
16 Dirkwin 4 5.00 1.00 15.00 25.00 8.25
19 Stacy 4 5.00 3.00 25.00 25.00 14.50
21 Mit 4 25.00 25.00 5.00 5.00 15.00
22 Wheeler 4 35.00 15.00 10.00 10.00 17.50
编号
No.		 品种
Variety		 苗期对混合
小种抗性
Seedling IT to
mix species		 2017保定
严重度1
FDS of Baoding
(%)		 2017保定
严重度2
FDS of Baoding
(%)		 2017周口
严重度1
FDS of Zhoukou
(%)		 2017周口
严重度2
FDS of Zhoukou
(%)		 均值
Average FDS
(%)
26 Saluda 4 5.00 20.00 15.00 15.00 13.75
27 Glenman 4 5.00 15.00 20.00 20.00 15.00
30 Copper 4 5.00 5.00 15.00 15.00 10.00
31 Century 4 5.00 20.00 20.00 20.00 16.25
33 Dodge 4 5.00 5.00 25.00 30.00 16.25
34 Williams 4 1.00 15.00 20.00 20.00 14.00
35 Lancer 4 5.00 5.00 15.00 25.00 12.50
36 Madsen 4 5.00 15.00 20.00 20.00 15.00
37 GR876 4 5.00 15.00 15.00 35.00 17.50
38 Tiber 4 1.00 15.00 10.00 30.00 14.00
39 Laura 4 1.00 1.00 30.00 30.00 15.50
41 Karl 4 5.00 5.00 15.00 35.00 15.00
43 Sharp 4 5.00 5.00 30.00 30.00 17.50
44 Wakefield 4 5.00 5.00 15.00 35.00 15.00
45 Hoff 4 5.00 20.00 20.00 20.00 16.25
46 Gene 4 1.00 1.00 1.00 20.00 5.75
47 Vista 4 1.00 20.00 5.00 30.00 14.00
48 AC Taber 4 1.00 20.00 10.00 25.00 14.00
50 McNeal 4 5.00 25.00 10.00 30.00 17.50
52 Lambert 4 10.00 10.00 20.00 20.00 15.00
53 Pomerelle 4 5.00 5.00 15.00 45.00 17.50
54 Cayuga 4 5.00 25.00 20.00 20.00 17.50
55 Boundary 4 10.00 10.00 15.00 25.00 15.00
56 Caledonia 4 5.00 30.00 15.00 15.00 16.25
57 Millennium 4 5.00 5.00 1.00 35.00 11.50
58 Jubilee 4 5.00 30.00 20.00 20.00 18.75
60 Avalanche 4 5.00 25.00 10.00 5.00 11.25
65 Alson 4 1.00 20.00 15.00 15.00 12.75
66 Steel-ND 4 15.00 15.00 20.00 10.00 15.00
68 Grandin 4 10.00 5.00 20.00 35.00 17.50
LSD 10.84
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