Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1439-1444.doi: 10.3724/SP.J.1006.2009.01439

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Differential Exprcssion of QM-Like Protein Homologue from Maize

WANG Hui-Wei,LI Hong-Jie,ZHU Zhen-Dong,WU Xiao-Fei,WANG Xiao-Ming*   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Beijing 100081,china
  • Received:2009-02-10 Revised:2009-03-14 Online:2009-08-12 Published:2009-06-10
  • Contact: WANG Xiao-Ming, E-mail: wangxm@mail.caas.net.cn

Abstract:

A full-length QM-like cDNA (designated ZmQM) was cloned from maize (Zea mays L.) leaf tissues using cDNA amplified fragment length polymorphism (cDNA-AFLP) and rapid amplification of cDNA ends (RACE) techniques. The expression of ZmQM was examined in leaves of the Ht2 isogenic lines Huangzaosi and HuangzaosiHt2 carrying gene Ht2 for resistance to northern corn leaf blight after inoculation with race 1 of Exserohilum turcicum (Pass.) Leonard et Suggs. Gene ZmQM contains an open reading frame 738 bp in length, which encodes 245 amino acids with a predicted molecular weight of 27.78 kD and an isoelectric point of 10.69. Scanning PROSITE motifs indicated that the amino acid sequence of ZmQM protein includes a Ribosomal protein Ll0e signature, an N-glycosylation site, four Protein kinase C phosphorylation site, a Casein kinase II phosphorylation site, a Tyrosine kinase phosphorylation site, an N-myristoylation site, and an Amidation site. The nucleotide sequence of ZmQM shared 66–92% identity to QM genes isolated from other species. RT-PCR analysis showed that the expression of gene ZmQM was up-regulated in Huangzaosi Ht2 at 12 h after inoculation with race 1 of E. turcicum compared with that in Huangzaosi. By inference, ZmQM protein may be involved in response of HuangzaosiHt2 to inoculation by E. turcicum race 1.

Key words: QM, Ribosomal protein L10, ZmQM, Huangzaosi Ht2, Exserohilu turcicum

[1] Hooker A L. A new type of resistance in corn to Helminthosporium turcicum in seedling corn.Plant Dis Rep, 1961, 45: 780-781

[2] Hooker A L. A second major gene locus in corn for chlorotic-lesion resistance to Helminthosporium turcicum 1. Crop Sci, 1977, 17: 132-135

[3] Hooker A L. Resistance to Helminthosporium turcicum from Tripsacum floridanum incorporated into corn. Maize Genet Coop Newsl, 1981, 55: 87-88

[4] Gevers O H A. A new major gene for resistance to Helminthosporium turcicum leaf blight of maize. Plant Dis Rep, 1975, 59: 296-299

[5] Robbins Jr W A, Warren H L. Inheritance of resistance to Exserohilum turcicum in PI 209135 “Mayorbela” variety of maize. Maydica, 1993, 38: 209-213

[6] Juliana B O, Marco A G, Isaias O G, Luis E. New resistance gene in the Zea mays- Exserohilum turcicum pathosystem. Genet Mol Biol, 2005, 28, 435-439

[7] Carson M L. A new gene in maize conferring the“chlortic halo” reaction to infection by Exserohilum turcicum. Plant Dis, 1995, 79: 717-720

[8] Wang Y-P(王玉萍), Wang X-M(王晓鸣), Ma Q(马青). Races of Exserohihun turcicum, causal agent of northern leaf blight in China. J Maize Sci(玉米科学), 2007, 15(2): 123-126

[9] Bachem C W B, van der Hoeven R S, de Bruijn S M, Vreugdenhil D, Zabeau M, Visser R G F. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development. Plant J, 1996, 9: 745-753

[10] Rodo A P, Brugiere N, Vankova R, Malbeck J, Olson J M, Haines S C, Martin R C, Habben J E, Mok D W S, Mok M C. Over-expression of a zeatin O-glucosylation gene in maize leads to growth retardation and tasselseed formation. J Exp Bot, 2008, 59: 2673-2686

[11] Farmer A A, Loftus T M, Mills A A, Sato K Y, Neill J D, Tron T, Yang M, Trumpower B L, Stanbridge E J. Extreme evolutionary conservation of QM, a novel c-Jun associated transcription factor. Human Mol Genet, 3: 723-728

[12] Chan Y L, Diaz J J, Denoroy L, Madjar J J, Wool I G. The primary structure of rat ribosomal protein L10: Relationship to a Jun-binding protein and to a putative Wilms’ tumor suppressor. Biochem Biophys Res Commun, 1996, 225: 952-956

[13] Wool I G. Extra ribosomal functions of ribosomal proteins. Trends Biochem Sci, 1996, 21: 164-165

[14] Rivera-Madrid R, Marinho P, Chartier Y, Meyer Y. Nucleotide sequence of an Arabidopsis thaliana cDNA clone encoding a homolog to a suppressor of Wilms’ tumor. Plant Physiol, 1993, 102: 329-330

[15] Chen C, Wanduragala S, Becker D F, Dickman M B. Tomato QM-like protein protects Saccharomyces cerevisiae cells against oxidative stress by regulating intracellular proline levels. Appl Environ Microbiol, 2006, 72: 4001-4006

[16] Singh K, Paul A, Kumar S, Ahuja P S. Cloning and differential expression of QM like protein homologue from tea

[Camellia sinensis (L.) O. Kuntze]. Mol Biol Rep, May 4, 2008, (Online) DOI: 10.1007/s11033-008-9264-x

[17] Rocha C S, Santos A A, Machado J P B, Fontes E P B. The ribosomal protein L10/QM-like protein is a component of the NIK-mediated antiviral signaling. Virology, 2008, 380: 165-169

[18] Berquist R R, Masias O R. Physiologic specialization in Thrichomes-tasphaeria turcica f. sp. zeae and T. turcica f. sp. sorghi in Hawaii. Phytopathology, 1974, 64: 645-649

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!