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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (06): 995-1002.doi: 10.3724/SP.J.1006.2010.00995

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of ADP-Glucose Pyrophosphorylase Activity on Sterility and Development of Grain in K, V, and T-Cytoplasmic Male Sterile Lines in Wheat

WU Shi-Wen1,GAO Qing-Rong1*,SUN Zhe1,2,WANG Mao-Ting1,SUN Zheng-Juan1,YUAN Kai1,YU Song1   

  1. 1 National Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai’an 271018, China; 2 Tai’an Academy of Agricultural Science, Tai’an 271000, China
  • Received:2010-01-19 Revised:2010-03-04 Online:2010-06-12 Published:2010-04-20
  • Contact: GAO Qing-Rong,E-mail:gaoqingr@sdau.edu.cn E-mail:wushiwen0036@163.com

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Abstract:

Cytoplasmic male-sterile (CMS) lines, especially K (Aegilops kotchyi), V (Aegilops ventricosa), and T (Triticum timopheevi) types of cytoplasms, are of high value in heterosis utilization in wheat (Triticum aestivum L.). The sterile mechanisms of these CMS types have been focused in hybrid wheat research. As a key and rate-limiting starch biosynthetic enzyme, ADP-glucose pyrophosphorylase (AGPase) is considered to play an important role in the sterility of CMS lines. For gaining an insight into the physiological basis of the sterility in CMS lines of wheat, the activities of AGPase in leaves, young spikes, and grains were measured in K, V, and T types of isogenic CMS lines (Ji 5418 background) at different growth stages, and compared with those of their maintainer line. The dynamic accumulations of starch, amylose, and amylopectin were also investigated in both superior (the first and the second grains in a spikelet) and inferior (grains except for the first and the second grains in a spikelet) grains. In young spikes, the AGPase activities of the three CMS lines were significantly higher (F = 133.81, P < 0.0001) with the differences of 9.33 - 27.94 μmol g-1 FW h-1 at floret primordium stage, and significantly lower (F = 13.97 - 75.20, P < 0.0001) by 4.27 - 7.44 μmol g-1 FW h-1 at tetrad stage as compared with the maintainer line. From floret primordium to tetrad stage, the CMS lines had continuously higher level of AGPase activities in leaves than the maintainer line, with the differences ranged from 7.39 to 80.77 μmol g-1 FW h-1 (F = 135.76 - 5454.28, P < 0.0001). Compared with the maintainer line, the three CMS lines all presented lower accumulations (P < 0.05) of starch, amylose and amylopectin in grain. The starch content and AGPase activity in superior grains were significantly higher than those in inferior grains for CMS lines. The dynamic accumulations of starch in superior and inferior grains fitted Logistic equations, and positively correlated with the starch accumulation rate (r = 0.4460 - 0.7150, P = 0.0004 - 0.0487). The AGPase activity in leaves had an insignificantly negative correlation with net photosynthetic rate. In conclusion, the male sterility is probably in relation to the insufficiency of energy in leaves and young pikes at floret primordium stage resulting from the high AGPase activity, and the low accumulation of starch in pollens at tetrad stage caused by low AGPase activity in young spikes. The sterile cytoplasm has a negative effect on AGPase activity, resulting in decreases of ADPG level and starch accumulation in grains. Besides, the negative effect of AGPase activity on net photosynthetic rate in flag leaves may result in unfilled grains in CMS lines.

Key words: Cytoplasmic male sterility (CMS), ADP-glucose pyrophosphorylase, Sterile mechanism, Isogenic CMS lines of wheat


[1] Kihara H. Substitution of nucleus and its effects on genome manifestations. Cytologia, 1951, 16: 177-193

[2] Zhang G-S(张改生), Yang T-Z(杨天章). A preliminary on the male sterile lines of wheat with Ae. ventricosa, Ae. kotschyi and Ae. variabilis cytoplasms. Acta Agron Sin (作物学报), 1989, 15(1): 1-10(in Chinese with English abstract)

[3] Gao Q-R(高庆荣), Liu B-S(刘保申), Sun L-Z(孙兰珍), Ji F-Q(纪复勤). The comparative study on cytoplasmic effect of K, V, A-cytoplasmic hybrid wheat. Tritical Crops (麦类作物), 1998, 18(4): 1-3(in Chinese)

[4] Guo R-X(郭瑞星), Shao R-X(邵仁学). Research progress of wheat hybrid heterosis utilization. Hubei Agric Sci (湖北农业科学), 1996, (6): 25-28 (in Chinese)

[5] Qiao L-X(乔利仙), Zhang G-S(张改生), Liu H-W(刘宏伟), Wang J-W(王军卫), Wang X-L(王小利). Studies on seed quality of hybids between some wheat male sterile lines and Ae. kotsehyi, Ae. variabilis, Ae. ventrieosa and Ae. bieornis cytoplasms. Sci Agric Sin (中国农业科学), 2001, 34(6): 587-591(in Chinese with English abstract)

[6] Zhu L-C(朱列层), Ma L-J(马翎建), Song X-Y(宋喜悦), Xi Y-J(奚亚军), Hu Y-G(胡银岗), Liu S-D(刘曙东), Yang C-Y(杨存义), He B-R(何蓓如). The comparative study on wheat CMS lines with Ae. uniaristata and Ae. kotschyi cytoplasm: II. Agronomic effect. Acta Univ Agric Boreali-Occident (西北农业大学学报), 1999, 27(1): 6-9(in Chinese with English abstract)

[7] Gao Q-R(高庆荣), Zhang A-M(张爱民), Wang R-X(王瑞霞), Qiao X-L(乔晓琳), Zhang Y-X(张永祥). The cytoplasmic effect on quality characters of K, T, V, CHA-cytoplasmic hybrid wheat. Acta Agron Sin (作物学报), 2005, 31(1): 43-47(in Chinese with English abstract)

[8] Gao Z(高忠), Zhang R-X(张荣铣), Zhao Y-H(赵寅槐). Studies on the effects of alloplasms from T. timopheevi and Ae. ventricosa on photosynthetic traits in wheat flag leaves. Sci Agric Sin (中国农业科学), 1996, 29(6): 29-37(in Chinese with English abstract)

[9] Yi Z-L(易自力), Xu N-Y(徐乃瑜). The effects of allocytoplasm on photosynthetic characterristics of common wheat. Nat Sci J Changsha Normal Univ Water Resour Electric Power (长沙水电师院自然科学学报), 1993, 8(1): 82-87(in Chinese with English abstract)

[10] Tsunewaki K, Yoshida T, Tsuji S. Genetic diversity of the cytoplasm in Triticum and Aegilops: IX. The effect of alien cytoplasms on seed germination of common wheat. Jpn J Genet, 1983, 58: 33-41

[11] Qiao X-L(乔晓琳), Gao Q-R(高庆荣), Zhang A-M(张爱民), Zhao G-Q(赵桂清), Liu Z-B(刘正斌), Qiu X-M(邱新民). Analysis of photosynthetic characteristics of K, V, T and CHA-cytoplasmic male sterile lines of wheat. Acta Agron Sin (作物学报), 2006, 32(9): 1323-1328(in Chinese with English abstract)

[12] Jiao J(焦健), Gao Q-R (高庆荣), Wang D-W(王大伟), Hao Y-Y(郝媛媛), Qiu X-M(邱新民), Jiang H(姜辉), Guo F-Z(郭凤芝). Analysis on factors affecting photosynthetic rate in different male sterility lines of wheat. Sci Agric Sin (中国农业科学), 2008, 41(6): 1622-1629(in Chinese with English abstract)

[13] Wu S-W(吴世文), Zhang S-Y(张淑英), Gao Q-R(高庆荣), Li L-J(李罗江), Jiang H(姜辉), Guo F-Z(郭凤芝), Sun Z(孙哲). Study on photosynthesis and physiological parameters in hybrids with different cytoplasmic male-sterile wheat lines. J Triticeae Crops (麦类作物学报), 2009, 29(2): 217-221(in Chinese with English abstract)

[14] Li Y-H(李玉红), Guo A-G(郭蔼光), Yang T-Z(杨天章). Comparative studies on ATPase isozyme of different type cytoplasmic male sterile lines and their maintainers in common wheat. Acta Agric Boreali-Occident Sin (西北农业学报), 2002, 11(1): 28-30(in Chinese with English abstract)

[15] Yao Y-Q(姚雅琴), Zhang G-S(张改生), Liu H-W(刘宏伟), Wang J-W(王军卫). Studies on correlation between intine and K-type cytoplasmic male sterility in wheat. Acta Bot Boreali-Occident Sin (西北植物学报), 2002, 22(2): 333-337(in Chinese with English abstract)

[16] Yao Y-Q(姚雅琴), Zhang G-S(张改生). Comparative studies of ATPase activity of K-type cytoplasmic male sterile wheat line and its maintainer. Sci Agric Sin (中国农业科学), 2000, 33(3): 97-99(in Chinese with English abstract)

[17]Yao Y-Q(姚雅琴), Zhang G-S(张改生), Liu H-W(刘宏伟), Wang J-W(王军卫). Ultracytochemical localization of ATPase and cytochrome oxidase in the anther from K-77(2)A and 77(2)B. Acta Agron Sin (作物学报), 2001, 27(1): 43-48(in Chinese with English abstract)

[18]Greene T W, Hannah L C. Enhanced stability of maize endosperm ADP-glucose pyrophosphorylase is grained through mutants that alter subunit interactions. Proc Natl Acad Sci USA, 1998, 95: 13342-13347

[19] Nakamura Y, Yuki K, Park S Y and Ohya T. Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiol, 1989, 56: 833-839

[20] Smyth D A, Prescott Jr H E. Sugar content and activity of sucrose metabolism enzymes in milled rice grain. Plant Physiol, 1989, 89: 893-896

[21] He Z-F(何照范). Analysis Technique for Grain Quality in Cereals and Oils (粮油籽粒品质及其分析技术). Beijing: Agriculture Press, 1985. pp 144-294 (in Chinese)

[22] Li W-Y(李文阳), Yin Y-P(尹燕枰), Yan S-H(闫素辉), Dai Z-M(戴忠民), Li Y(李勇), Liang T-B(梁太波), Geng Q-H(耿庆辉), Wang Z-L(王振林). Effect of shading after anthesis on starch accumulation and activities of the related enzymes in wheat grain. Acta Agron Sin (作物学报), 2008, 34(4): 632-640(in Chinese with English abstract)

[23] Ballicora1 M A, Iglesias A A, Preiss J. ADP-glucose pyrophosphorylase: a regulatory enzyme for plant starch synthesis. Photosynthesis Res, 2004, 79: 1-24

[24] Zhong Yi Li, Xiu Sheng Chu, Mouille G, Liu Ling Yan, Kosar-Hashemi B, Hey S, Napier J, Shewry P, Clarke B, Appels R, Morell M. K, Rahman S. The localization and expression of the class: II. Starch synthases of wheat. Plant Physiol, 1999, 120: 1147-1156

[25] Guo T-C(郭天财), Wang Z-J(王之杰), Hu T-J(胡廷积), Zhu Y-J(朱云集), Wang C-Y(王晨阳), Wang H-C(王化岑), Wang Y-H(王永华). Study on canopy apparent photosynthesis characteristics and grain yield traits of two winter wheat cultivars with different spike type. Acta Agron Sin (作物学报), 2001, 27(5): 633-639 (in Chinese with English abstract)

[26] Jiang D(姜东), Yu Z-W(于振文), Li Y-G(李永庚), Yu S-L(余松烈). Effects of different nitrogen application levels on changes of sucrose content in leaf, culm, grain and photosynthate distribution and grain starch accumulation of winter wheat. Sci Agric Sin (中国农业科学), 2002, 35(2): 157-162(in Chinese with English abstract)

[27] Wang C-Y(王晨阳), Ma D-Y(马冬云), Zhu Y-J(朱云集), Guo T-C(郭天财), Feng W(冯伟), Zhou S-M(周苏玫). Effects of different irrigation and nitrogen application regimes in winter wheat on cooking qualities of Chinese noodle. Sci Agric Sin (中国农业科学), 2004, 37(2): 256-262 (in Chinese with English abstract)

[28] Mccormick K M, Panozzo J F, Hong S H. A swelling power test for selecting potential noodle quality wheats. Aust J Agric Res, 1991, 42: 317-323
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