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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1735-1742.doi: 10.3724/SP.J.1006.2011.01735

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Analysis of AGAMOUS Homologous Gene and Its Promoter from Vicia sativa L.

ZHANG Lei,LIU Zhi-Peng,WANG Yan-Rong*   

  1. Key Laboratory of Pastoral Agriculture System / School of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Received:2011-03-01 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 王彦荣, E-mail: wangyrlzu@126.com E-mail:planta@Yeah.net

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Abstract: Common vetch (Vicia sativa L.) is considered to be an idealliquidity spring proteid forage for Tibet stockbreeding. However, little has been known about the molecular mechanism of floral organ formation and subsequent seed development process during domestication of common vetch in alpine steppe. AGAMOUS(AG)gene is one of the most important floral homologous genes involved in plant stamen and gynoecium development. In this study, an AG homologue gene and its upstream regulation sequence were isolated from Vicia sativa L. using hiTAIL-PCR, RACE, and RT-PCR. The results showed that this gene was 3 158 bp in DNA sequence, coding a protein product of 244 animo acides. Homological analysis showed that the similarity between this sequence and Pisum sativum PsAG gene was over 98% in animo acid level. Therefore, the sequence was considered to be Vicia sativa AG gene. It was named VsAG with the accession number JF313850 in GenBank. By analysing the cis-elements existing in upstream promoter region and the second intron, the results showed VsAG second intron region shared some similar cis-elements with promoter region. It indicated the second intron of VsAG gene may play an important role in gene expression regulation.

Key words: AGAMOUS(AG), Vicia sativa, Promoter clone, TAIL-PCR

[1]Nan Z-B(南志标), Zhang J-Y(张吉宇), Wang Y-R(王彦荣), Li C-J(李春杰), Nie B(聂斌), Zhang J-Q(张健全), Zhao H(赵宏). Genotype×environmet interactions and consistency analysis for agronomic characteristics of five Vicia sativa lines. Acta Ecol Sin (生态学报), 2004, 24: 395–401 (in Chinese with English abstract)
[2]Wang Y-W(王赟文), Nan Z-B(南志标), Wang Y-R(王彦荣), Li C-J(李春杰), Ma L-X(马隆喜). Herbage and seed yields of Vica and Lathyrus species under alpine grassland conditions. Acta Pratac Sin (草业学报), 2001, 10: 47–55 (in Chinese with English abstract)
[3]Hu X-W(胡小文), Wang Y-R(王彦荣), Nan Z-B(南志标), Nie B(聂斌). Sowing date effects on vetch seed quality. Acta Ecol Sin (生态学报), 2004, 24: 409–413 (in Chinese with English abstract)
[4]Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353: 31–37
[5]Theissen G, Saedler H. Fl oral quartets. Nature, 2001, 409: 469–471
[6]Honma T, Goto K. Complexes of MADS-box proteins are sufficient to convert leaves into floral organs. Nature, 2001, 409: 525–529
[7]Yanfsky M F, Ma H, Bowman J H. The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature, 1990, 346: 35–39
[8]Busch M A, Bomblies K, Weigel D. Activation of a floral homeotic gene in Arabidopsis. Science, 1999, 285: 585–587
[9]Lohmann J, Hong R, Hobe M, Hobe, M, Busch, M, Parcy, F, Simon, R, Weigel, D. A molecular link between stem cell regulation and floral patterning in Arabidopsis. Cell, 2001, 105: 793–803
[10]Pinyopich A, Ditta G S, Savidge B, Liljegren S J. Assessing the redundancy of MADS-box genes during carpel and ovule development. Nature, 2003, 424: 85–88
[11]Liljegren S J, Ditta G S, Eshed Y, Savidge B, Bowman J H, Yanofsky M F. SHATTERPROOF MADS-box genes control seed dispersal in Arabidopsis. Nature, 2000, 404: 766–700
[12]Hong R L, Hamaguchi L, Busch M A, Weigel D. Regulatory elements of the floral homeotic gene AGAMOUS identified by phylogenetic footprinting and shadowing. Plant Cell, 2003, 15: 1296–1309
[13]Liu Y G, Chen Y L. High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. BioTechniques, 2007, 43: 649-656
[14]Busch A, Bomblies K, Weigel D. Activation of a floral homeotic gene in Arabidopsis. Science, 1999, 285: 585–587
[15]Sieburthl E, Meyerowitze M. Molcular dissection of the AGAMOUS control region shows that cis-elements for spatial regulation are located in tragenically. Plant Cell, 1997, 9: 355–365
[16]Jack T, Sieberth L, Meyerowit Z E. Target misex pression of AGAMOUS in whorl 2 of Arabidopsis flowers. Plant J, 1997, 11: 825–839
[17]Folter S, Immink R G, Kieffer M, Parenicova L. Comprehensive interaction map of the Arabidopsis MADS-box transcription factors. Plant Cell, 2005, 17: 1424–1433
[18]Hecht V, Foucher F, Ferrandiz C, Macknight R, Navarro C, Morin J. Conservation of Arabidopsis flowering genes in model Legumes. Plant Physiol, 2005, 137: 1420–1434
[19]Xu L(徐雷), Song W-J(宋伟杰), Wang L-L(王利琳). Preliminary study on the functions of AGAMOUS homologous genes in Pisum sativum . Chin Sci Bull (科学通报), 2009, 54: 3207–3212 (in Chinese)
[20]Pylatuik J D, Cross R H, Davis A R, Bonham P C. Elements regulating AGAMOUS expression are conserved between Arabidopsis thaliana, Brassica napus and Linum usitatissimum. Canad J Bot, 2003, 81: 523–530
[21]Chen Y(陈瑶), Xu Y(徐勇), Ma R-C(马荣才). Cloning and identification of the second intron of an AGAMOUS-like gene from peach (Prunus persica). China Biotechnol (中国生物工程杂志), 2008, 28: 104–110 (in Chinese with English abstract)
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