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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1735-1742.doi: 10.3724/SP.J.1006.2011.01735

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Analysis of AGAMOUS Homologous Gene and Its Promoter from Vicia sativa L.

ZHANG Lei,LIU Zhi-Peng,WANG Yan-Rong*   

  1. Key Laboratory of Pastoral Agriculture System / School of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Received:2011-03-01 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 王彦荣, E-mail: wangyrlzu@126.com E-mail:planta@Yeah.net

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Abstract: Common vetch (Vicia sativa L.) is considered to be an idealliquidity spring proteid forage for Tibet stockbreeding. However, little has been known about the molecular mechanism of floral organ formation and subsequent seed development process during domestication of common vetch in alpine steppe. AGAMOUS(AG)gene is one of the most important floral homologous genes involved in plant stamen and gynoecium development. In this study, an AG homologue gene and its upstream regulation sequence were isolated from Vicia sativa L. using hiTAIL-PCR, RACE, and RT-PCR. The results showed that this gene was 3 158 bp in DNA sequence, coding a protein product of 244 animo acides. Homological analysis showed that the similarity between this sequence and Pisum sativum PsAG gene was over 98% in animo acid level. Therefore, the sequence was considered to be Vicia sativa AG gene. It was named VsAG with the accession number JF313850 in GenBank. By analysing the cis-elements existing in upstream promoter region and the second intron, the results showed VsAG second intron region shared some similar cis-elements with promoter region. It indicated the second intron of VsAG gene may play an important role in gene expression regulation.

Key words: AGAMOUS(AG), Vicia sativa, Promoter clone, TAIL-PCR

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