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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (5): 693-704.doi: 10.3724/SP.J.1006.2019.84120

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of drought resistance and DNA methylation level of resynthesized Brassica napus

Yi YUAN,Shuang ZHU,Ting-Ting FANG,Jin-Jin JIANG(),You-Ping WANG   

  1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu, China
  • Received:2018-09-09 Accepted:2018-12-24 Online:2019-05-12 Published:2019-01-17
  • Contact: Jin-Jin JIANG E-mail:jjjiang@yzu.edu.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31771824);This study was supported by the National Natural Science Foundation of China(31771825);China and Jiangsu Postdoctoral Science Foundation(2014M561719);China and Jiangsu Postdoctoral Science Foundation(2015T80591);China and Jiangsu Postdoctoral Science Foundation(1401078B)

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Abstract:

Brassica napus, as one of the important resources of edible plant oil and forage protein, is a polyploid species with great economic value. However, it is sensitive to drought stress throughout whole lifecycle due to short domestication history and narrow genetic background. Thus, it is a main purpose to breed B. napus cultivar with high yield and drought resistance. In the present study, we compared the drought resistance among S1-S4 generations of resynthesized B. napus and diploid parents under different time periods of 15% PEG-6000 treatment. The different drought tolerance levels were assessed based on phenotype observation, leaf physiological indexes (MDA, soluble protein, SOD and POD). Accompanying with water content analysis, we found the drought tolerance showed a trend of B. oleracea > Bn-S3 > Bn-S4 > Bn-S1 > Bn-S2 > B. rapa. Under drought stress, POD and SOD activities in Bn-S3 and Bn-S4 were higher than these in other plants tested, and MDA content was decreased, indicating that Bn-S3 and Bn-S4 have better ability in clearing ROS, and defending from peroxidation damage. On the basis of HPLC analysis, the methylation level in all materials was the highest under drought stress of 12 h. And the methylation level in B. rapa was higher than that in others, that in Bn-S1 and Bn-S4 was between that in parents, while that in Bn-S2 and Bn-S3 was lower than that in parents. Methylation sensitive amplification polymorphism analysis also revealed multiple changes in methylation and demethylation level of resynthesized B. napus under drought stress, indicating methylation changes might be involved in plant drought tolerance.

Key words: resynthesized Brassica napus, B. rapa, B. oleracea, drought stress, DNA methylation

Fig. 1

Phenotype of plants under drought stress Br: B. rapa; Bo: B. oleracea; S1-S4: progenies of resynthesized B. napus."

Fig. 2

Relative water content and relative water retention in plants under drought stress of 12 h CK: control; DT: drought stress; Br: B. rapa; Bo: B. oleracea; S1-S4: progenies of resynthesized B. napus."

Fig. 3

Physiological indexes of plants under drought stress A: POD activity; B: MDA content; C: SOD activity; D: soluble protein. Average value ± SD, n = 3. Br: B. rapa; Bo: B. oleracea; S1-S4: progenies of resynthesized B. napus."

Fig. 4

Variation of DNA methylation level of plants under drought stress Br: B. rapa; Bo: B. oleracea; Bn-S1-S4: progenies of resynthesized B. napus."

Fig. 5

Percentage of different DNA methylations in plants under drought stress Br: B. rapa; Bo: B. oleracea; Bn-S1-S4: progenies of resynthesized B. napus."

Table 1

Variation of DNA methylation levels in plants under drought stress of 6h"

Table 2

Variation of DNA methylation levels in plants under drought stress of 12h"

Table 3

Variation of DNA methylation levels in plants under drought stress of 24h"

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