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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (4): 484-490.doi: 10.3724/SP.J.1006.2020.94112

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development of novel peanut genotypes with resistance to bacterial wilt disease, large pod, and high shelling percentage

LI Wei-Tao,XU Zhi-Jun,CAI Yan,GUO Jian-Bin,YU Bo-Lun,HUANG Li,CHEN Yu-Ning,ZHOU Xiao-Jing,LUO Huai-Yong,LIU Nian,CHEN Wei-Gang,REN Xiao-Ping,JIANG Hui-Fang()   

  1. Oil Crops Research Institute, China Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
  • Received:2019-08-04 Accepted:2019-12-26 Online:2020-04-12 Published:2020-01-15
  • Contact: Hui-Fang JIANG E-mail:peanutlab@oilcrops.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31601340);This study was supported by the National Natural Science Foundation of China(31571713);This study was supported by the National Natural Science Foundation of China(31761143005);This study was supported by the National Natural Science Foundation of China(31461143022)

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Abstract:

Bacterial wilt is an important soil-borne bacterial disease effecting yield and quality of peanut. Hundred pod weight and shelling percentage are also related to peanut yield. In this study, the major QTL qBWRB02 related to bacterial wilt resistance was identified by using the RIL population derived from the cross between Yuanza 9102 and Xuzhou 68-4. Combining with the QTL mapping results for shelling percentage and hundred pod weight in previous research, it was found that the major QTL for these three traits were distributed on different chromosomes. Based on genotype data of RIL population and phenotypic data of bacterial wilt resistance, hundred pod weight and shelling percentage in multi-environment, We screened out six novel accessions with resistance to bacterial wilt disease, large pod, and high shelling percentage from RIL population by molecular markers closely linked with major QTL, which can be used as breeding intermediates or parents in peanut breeding for high yield and high disease resistance. The results of effectively screening disease-resistant and high-yield germplasm through the combination of molecular marker-assisted selection and phenotypic identification provide a new idea for peanut breeding in the future.

Key words: novel peanut genotype, bacterial wilt diseases resistance, hundred pod weight, shelling percentage

Table 1

Information of QTLs for resistance to bacterial wilt (BWR) using QTLNetwork 2.0"

QTL名称
QTL name		 环境
Environment		 范围
Range (cM)		 加性效应
Additive effect		 贡献率
PVE (%)
(A)		 贡献率
PVE (%)
(AE)
qBWRA01.1 2013HA 17.4-18.7 -9.5732 16.57
qBWRA01.2 ME 17.4-18.7 -6.16 8.77 0.52
(qBWRA01)
qBWRB02.1 2013HA 91.5-92.5 -15.95 21.45
qBWRB02.2 2014NC 91.5-92.5 -19.51 27.76
qBWRB02.3 2015NC 91.5-92.5 -21.18 45.21
qBWRB02.4 ME 91.5-92.5 -18.44 27.87 0.22
(qBWRB02)
qBWRB05 ME 4.5-8.5 5.66 2.54 0.79
qBWRB10.1 2013HA 87.9-98.9 -14.79 8.36
qBWRB10.2 2015NC 87.9-101.9 -11.81 6.49
qBWRB10.3 ME 90.9-100.9 -10.30 3.80 0.35
(qBWRB10)

Fig. 1

Location of QTLs for BWR in the genetic maps QTL surrounded by rectangle represents major QTL."

Table 2

Genotypes of AHGS2344 in breeding lines and cultivars with different resistance to bacterial wilt"

材料 Material 亲本 Parent AHGS2344 表型 Phenotype
远杂9102 Yuanza 9102 — A 抗 Resistant
中花21 Zhonghua 21 远杂9102 Yuanza 9102 A 抗 Resistant
J8 远杂9102 Yuanza 9102 A 抗 Resistant
J11 远杂9102 Yuanza 9102 A 抗 Resistant
J64 远杂9102 Yuanza 9102 A 抗 Resistant
J91 远杂9102 Yuanza 9102 A 抗 Resistant
J92 远杂9102 Yuanza 9102 A 抗 Resistant
J12 远杂9102 Yuanza 9102 B 不抗 Susceptible
中花6号 Zhonghua 6 — A 抗 Resistant
QT1319 中花6号Zhonghua 6 A 抗 Resistant
QT1315 中花6号Zhonghua 6 A 抗 Resistant
QT1299 中花6号Zhonghua 6 B 不抗 Susceptible
QT1322 中花6号Zhonghua 6 B 不抗 Susceptible
QT1306 中花6号Zhonghua 6 B 不抗 Susceptible
QT1314 中花6号Zhonghua 6 B 不抗 Susceptible
徐花13 Xuhua 13 — B 不抗 Susceptible
徐州68-4 Xuzhou 68-4 — B 不抗 Susceptible

Table 3

Novel genotypes with resistance to bacterial wilt disease, large pod and high shelling percentage selected by molecular markers tightly linked to QTL"

家系
Line		 青枯病抗性(qBWB02)
Resistance to bacterial wilt (qBWB02)		 百果重(qHPWA05)
Hundred pod weight (qHPWA05)		 出仁率(qSPA09)
Shelling percentage (qSPA09)
AhTE0775 AHGS2344 均值
Mean (%)		 AD05A20499 Ad05A20262 均值
Mean (g)		 AGGS0957 GNB652 均值
Mean (%)
QT0718 A A 81.89 B B 239.09 A A 81.77
QT0719 A A 80.36 B B 257.07 A A 80.19
QT0748 A A 92.88 B B 238.22 A A 83.68
QT0752 A A 94.89 B B 228.44 A A 79.41
QT0793 A A 88.10 B B 235.49 A A 81.63
QT0845 A A 88.89 B B 222.45 A A 80.83
徐州68-4
Xuzhou 68-4		 B B 20.00 B B 251.84 B B 76.93
远杂9102
Yuanza 9102		 A A 89.80 A A 187.43 A A 81.84
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